Culture media and uses Flashcards
1
Q
Alkaline Peptone Broth
A
Vibrio
2
Q
BIle Esculin Agar
A
Enterococci and Group D streptococci
3
Q
Bismuth Sulfite Agar
A
Selective media for Salmonella (Stool)
4
Q
Blood Agar
A
Differentiation of Hemolytic patterns and for Fastidious organism
5
Q
Bordet Gengou Agar
A
Bordetella Pertusis
6
Q
Brilliant Green Agar
A
Selective media for Salmonella
7
Q
Blood cystine Dextrose Agar
A
Francisella tularensis
8
Q
Buffered Charcoal Yeast Extract (BCYE)
A
Legionella spp.
9
Q
Campy-Blood Agar
A
Campylobacter spp.
10
Q
Campy-thioglycollate broth
A
Selective holding medium for Campylobacter, For cold enrichment (4) technique
11
Q
CDC anaerobe 5% sheep blood agar
A
Obligate, slow-growing anaerobe
12
Q
Cefoperazone Vancomycin Amphotericin Agar
A
Campylobacter spp.
13
Q
Cefsulodin-Irgasan-Novobiocin Agar (CIN)
A
Yersinia enterocolitica and Aeromonas
14
Q
Cetrimide Agar
A
Pseudomonas aeruginosa
15
Q
Chick embryo
A
Chlamydia and Rickettsia
16
Q
Chocolate Agar
A
Neisseria and Haemophilus
17
Q
Chocolate agar with horse blood
A
Haemophilus spp
18
Q
Chromogenic media
A
MRSA and fungi
19
Q
Columbia Colistin-Nalidixic Agar (CNA)
A
Selective for Gram-positive cocci
20
Q
Cystine Tellurite Blood Agar
A
Corynebacterium diptheriae
21
Q
Cyclosporine cefoxitin fructose agar
A
Clostridium difficile
22
Q
Czapek Agar
A
Nocardia asteroides
23
Q
Dorset egg medium
A
Mycobacterium tuberculosis, Nocardia asteroides
24
Q
Desoxycholate Citrate Agar
A
Salmonella and Shigella
25
Dieudonne's Medium
Vibrio cholerae
26
Dubos Oleic Agar
Mycobacterium tuberculosis
27
Edward Hayflick Agar
Mycoplasma
28
Eosin Methylene Blue (EMB) - Levine Medium
Differential media for LF and NLF
29
Ellinghausen, McCullough, Johnson and Harris (EMJH) medium
Leptospira interrogans
30
FLetcher's Semi solid medium
Leptospira
31
Fetal Bovine Serum Agar with vancomycin
Haemophilus ducreyi
32
Foot pads of mice
Mycobacterium leprae
33
Gram Negative (GN) Broth
For enteric pathogens like Salmonella and Shigella
34
Human Blood Bilater Tween medium
Gardnerella vaginalis
35
Hektoen Enteric (HE) Agar
Differentiation of Salmonella and Shigella
36
Kanamycin-Vancomycin Blood Agar(KVBA)
Bacteroides
37
Kelly's Medium
Borrelia burgdorferi
38
Loeffler's Blood Serum Medium
Corynebacterium diphtheriae
39
Lombard Dowell Medium
Anaerobic organisms
40
Lowenstein Jensen (LJ) Agar
Mycobacteria
41
Lysine Iron Agar
Enterobacteriaceae (deamination or decarboxylation)
42
Litmus Milk media
Clostridium spp.
43
Mac Conkey agar
Differential medium for LF and NLF, Selective for Gram-Negative bacteria
44
Mac Conkey Agar with Sorbitol
E. coli O157:H7 in fecal specimen
45
Mannitol Salt Agar
Staphylococcus aureus
46
Mc Bride Medium
Listeria monocytogenes
47
Middlebrook 7H10
Mycobacteria
48
Martin Lewis Agar
Neisseria gonorrhoeae
49
Mueller Hinton Agar
Susceptibility test (antimicrobial testing)
50
Methylene blue milk
Enterococci
51
New York city (NYC) Agar
Neisseria gonorrhoeae
52
Oxidative-Fermentative (OF) medium
Differential media for Acinetobacter, Alcaligenes, Pseudomonas
53
Petragnani Medium
Mycobacteria
54
Phenyl Ethyl Alcohol (PEA) Medium
For Gram positive bacteria
55
Rabbit Blood Agar with yeast extract
Haemophilus influenzae
56
Regan Lowe
Bordetella pertussis
57
Salmonella-Shigella Agar (SSA)
Salmonella and shigella
58
Seller's Agar
Pseudomonas aeruginosa
59
Selenite broth (Enrichment medium)
Salmonella spp.
60
Schaedler Agar
Nonselective medium for aerobes and anaerobes; Selective for campylobacter and Helicobacter
61
Skirrow agar
Campylobacter spp.
62
Tetrathionate Broth
Salmonella and Shigella spp.
63
Thayer Martin Agar
Neisseria gonorrhoeae and Neisseria meningitidis
64
Thioglycollate
Aerobes and anaerobes
65
Thiosulfate Citrate Bile Salt Sucrose Agar (TCBS)
Vibrio
66
Tinsdale Agar
Corynebacterium diptheriae
67
Todd-Hewitt Broth (selective and enrichment) With nalidixic acid and gentamicin/colistin
Streptococcus agalactiae in female genital specimens
68
Trypticase Soy Broth (TSB)
Blood culture bottle base Streptococcus pneumoniae, Brucella
69
Tyrosine Agar
Aerobic Actinomycetes
70
Tween 80-Albumin Broth
Spirochetes
71
Xylose Lysine Desoxycholate (XLD) Agar
Salmonella and Shigella
72
Maintain the viability of organisms during specimen transport
Transport Media
73
For Neisseria; Cary Blair-for stool pathogens like Vibrio
JEMBEC
74
Viral Transport Media
STUART'S
75
Media for Antibiotic Susceptibility Testing
Mueller Hinton Agar
76
Used in biochemical tests for species identification (TSI, LIA, Urea agar, SIM, Simmon Citrate agar, MRVP broth)
BIOCHEMICAL TEST MEDIA
77
Detects organism's motility (e.g., SIM, MIO)
MOTILITY MEDIUM
78
Supports the growth of anaerobes (e.g., Chopped meat media, Thioglycolate)
ANAEROBIC MEDIA
79
Transport media, maintains viability of organisms during transport
Amie's Media
80
Enrichment media for Vibrio
Alkaline Peptone water
81
Selective and differential media for Bacteroides
Bacteroides Bile Esculin
82
Differential isolation and identification of Group D Streptococci and Enterococci
Bile Esculin Agar
83
Selective for Vancomycin resistant enterococci
Bile Esculin Azide agar with Vancomycin
84
High protein media for growing a variety of bacteria
Brain Heart Infusion (BHI)
85
Isolation of Salmonella typhi
Bismuth Sulfite Agar
86
Cultivation of fastidious microorganisms and determination of haemolytic reactions
Blood Agar (BA)
87
For Francisella tularensis
Blood Cystine Dextrose Agar
88
Isolation of Bordetella pertussis
Bordet-Gengou Media
89
Selective media for Salmonella (except S. typhi)
Brilliant Green Agar (BGA)
90
Enrichment and selective for Legionella spp.
Buffered Charcoal Yeast Extract Agar with Antibiotics
91
Selective media for Staphylococcus
Chapman Stone agar
92
Selective for Campylobacter spp
Campy-Blood Agar
93
Selective holding medium for recovery of Campylobacter spp
Campylobacter Thioglycollate Broth
94
Transport media
Cary and Blair Media
95
Used for separating Nocardia & Streptomyces
Casein Media
96
Selective media for Campylobacter spp
Cefoperazone, Vancomycin, Amphotericin
97
Selective for Yersinia spp.
Cefsulodin-Irgasan-Novobiocin
98
Cultivation of Haemophilus spp. and pathogenic Neisseria
Chocolate Agar
99
Used for isolation of anaerobes, especially Clostridium spp.
Chopped Meat Glucose
100
Selective isolation of gram positive cocci
Columbia Colistin Nalidixic Acid Agar
101
Used for Chlamydospore production of Candida albicans
Corn Meal Agar with 1% Tween 80
102
Isolation of Corynebacterium diphtheriae
Cystine Tellurite Blood Agar
103
For determining fermentation reactions of Neisseria spp.
Cystine Trypticase Agar
104
Media for Clostridium difficile
Cycloserine Cefoxitin Fructose Agar
105
Differentiation of lactose fermenters from non-lactose fermenters
Deoxycholate Agar
106
For isolation of Salmonella and Shigella
Deoxycholate Citrate
107
Selective media for Vibrios
Dieudonnes Media
108
Isolation of Mycoplasma and Ureaplasma spp.
Edward Hayflick Agar
109
Used for isolation and identification of Clostridium
Egg Yolk Agar
110
Selective media for Leptospira interrogans
Ellinghausen, McCullough Johnson and Harris Media
111
Selective media for Haemophilus ducreyi
Fetal Bovine Serum with Vancomycin
112
For isolation of Haemophilus influenzae
Fildes Enrichment
113
Selective enrichment for enteric pathogens
Gram Negative Broth
114
Selective and differential for Salmonella and Shigella
Hektoen Enteric Agar
115
Selective media for Gardnerella vaginalis
Human Blood Bilayer Tween
116
For C. diphtheriae
Leoffler's Coagulated Serum
117
For isolation of Mycobacterium tuberculosis
Lowenstein Jensen Media
118
For selection and differentiation of E. coli O157:H7
Mac Conkey Sorbitol Agar
119
Selective for Staphylococci
Mannitol Salt Agar
120
For isolation of Listeria monocytogenes
Mc Bride Media
121
For isolation of Mycobacterium tuberculosis
Middelbrook 7H10 or 7H11
122
Selective media for Borrelia species
Modified Kelly's Medium
123
Used in Methyl Red and Vogues Proskauer tests
MRVP broth
124
Selective for Neisseria gonorrhoeae
New York City Agar
125
Similar to Lowenstein Jensen, contains more malachite green
Petragnani's Media
126
Selective isolation of gram positive cocci and anaerobic gram negative bacilli
Phenylethyl Alcohol Agar
127
Enrichment and selective media for Bordetella pertussis
Regan Lowe
128
Used for fungus culture
Sabouraud Dextrose Maltose Agar
129
Selective for Salmonella and Shigella
Salmonella-Shigella Agar
130
Non-selective for aerobes and anaerobes
Schaedler Agar
131
Enrichment for Salmonella spp.
Selenite Broth
132
Test for citrate as a carbon source
Simmon Citrate Agar
133
Selective for Campylobacter spp.
Skirrow Agar
134
Selective for Streptococcus agalactiae
Streptococcal Selective Agar
135
Selective for Salmonella and Shigella spp.
Tetrathionate Broth
136
Selective for Neisseria gonorrhoeae and Neisseria meningitidis
Thayer Martin Agar
137
Supports growth of anaerobes, aerobes, microaerophilic microorganisms
Thioglycollate Broth
138
Selective and differential for Vibrios
Thiosulfate Citrate Bile Salts Agar
139
Selective and enrichment for Streptococcus agalactiae
Todd Hewitt Broth supplemented with antibiotics
140
Enrichment for subculturing bacteria
Trypticase Soy Broth
141
Biochemical tests for differentiating enteric bacilli
Triple Sugar Iron Agar
142
For isolation and differentiation of Salmonella and Shigella
Xylose Lysine Desoxycholate Agar
143
Used for urease test, identifies urease producers like Proteus
Urea Agar
