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BACTERIOLOGY > Culture media and uses > Flashcards

Culture media and uses Flashcards

1
Q

Alkaline Peptone Broth

A

Vibrio

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2
Q

BIle Esculin Agar

A

Enterococci and Group D streptococci

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3
Q

Bismuth Sulfite Agar

A

Selective media for Salmonella (Stool)

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4
Q

Blood Agar

A

Differentiation of Hemolytic patterns and for Fastidious organism

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5
Q

Bordet Gengou Agar

A

Bordetella Pertusis

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6
Q

Brilliant Green Agar

A

Selective media for Salmonella

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7
Q

Blood cystine Dextrose Agar

A

Francisella tularensis

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8
Q

Buffered Charcoal Yeast Extract (BCYE)

A

Legionella spp.

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9
Q

Campy-Blood Agar

A

Campylobacter spp.

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10
Q

Campy-thioglycollate broth

A

Selective holding medium for Campylobacter, For cold enrichment (4) technique

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11
Q

CDC anaerobe 5% sheep blood agar

A

Obligate, slow-growing anaerobe

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12
Q

Cefoperazone Vancomycin Amphotericin Agar

A

Campylobacter spp.

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13
Q

Cefsulodin-Irgasan-Novobiocin Agar (CIN)

A

Yersinia enterocolitica and Aeromonas

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14
Q

Cetrimide Agar

A

Pseudomonas aeruginosa

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15
Q

Chick embryo

A

Chlamydia and Rickettsia

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16
Q

Chocolate Agar

A

Neisseria and Haemophilus

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17
Q

Chocolate agar with horse blood

A

Haemophilus spp

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18
Q

Chromogenic media

A

MRSA and fungi

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19
Q

Columbia Colistin-Nalidixic Agar (CNA)

A

Selective for Gram-positive cocci

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20
Q

Cystine Tellurite Blood Agar

A

Corynebacterium diptheriae

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21
Q

Cyclosporine cefoxitin fructose agar

A

Clostridium difficile

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22
Q

Czapek Agar

A

Nocardia asteroides

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23
Q

Dorset egg medium

A

Mycobacterium tuberculosis, Nocardia asteroides

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24
Q

Desoxycholate Citrate Agar

A

Salmonella and Shigella

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25
Q

Dieudonne’s Medium

A

Vibrio cholerae

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26
Q

Dubos Oleic Agar

A

Mycobacterium tuberculosis

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27
Q

Edward Hayflick Agar

A

Mycoplasma

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28
Q

Eosin Methylene Blue (EMB) - Levine Medium

A

Differential media for LF and NLF

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29
Q

Ellinghausen, McCullough, Johnson and Harris (EMJH) medium

A

Leptospira interrogans

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30
Q

FLetcher’s Semi solid medium

A

Leptospira

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31
Q

Fetal Bovine Serum Agar with vancomycin

A

Haemophilus ducreyi

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32
Q

Foot pads of mice

A

Mycobacterium leprae

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33
Q

Gram Negative (GN) Broth

A

For enteric pathogens like Salmonella and Shigella

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34
Q

Human Blood Bilater Tween medium

A

Gardnerella vaginalis

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35
Q

Hektoen Enteric (HE) Agar

A

Differentiation of Salmonella and Shigella

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36
Q

Kanamycin-Vancomycin Blood Agar(KVBA)

A

Bacteroides

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37
Q

Kelly’s Medium

A

Borrelia burgdorferi

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38
Q

Loeffler’s Blood Serum Medium

A

Corynebacterium diphtheriae

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39
Q

Lombard Dowell Medium

A

Anaerobic organisms

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40
Q

Lowenstein Jensen (LJ) Agar

A

Mycobacteria

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41
Q

Lysine Iron Agar

A

Enterobacteriaceae (deamination or decarboxylation)

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42
Q

Litmus Milk media

A

Clostridium spp.

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43
Q

Mac Conkey agar

A

Differential medium for LF and NLF, Selective for Gram-Negative bacteria

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44
Q

Mac Conkey Agar with Sorbitol

A

E. coli O157:H7 in fecal specimen

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45
Q

Mannitol Salt Agar

A

Staphylococcus aureus

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46
Q

Mc Bride Medium

A

Listeria monocytogenes

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47
Q

Middlebrook 7H10

A

Mycobacteria

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48
Q

Martin Lewis Agar

A

Neisseria gonorrhoeae

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49
Q

Mueller Hinton Agar

A

Susceptibility test (antimicrobial testing)

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50
Q

Methylene blue milk

A

Enterococci

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51
Q

New York city (NYC) Agar

A

Neisseria gonorrhoeae

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52
Q

Oxidative-Fermentative (OF) medium

A

Differential media for Acinetobacter, Alcaligenes, Pseudomonas

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53
Q

Petragnani Medium

A

Mycobacteria

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54
Q

Phenyl Ethyl Alcohol (PEA) Medium

A

For Gram positive bacteria

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55
Q

Rabbit Blood Agar with yeast extract

A

Haemophilus influenzae

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56
Q

Regan Lowe

A

Bordetella pertussis

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57
Q

Salmonella-Shigella Agar (SSA)

A

Salmonella and shigella

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58
Q

Seller’s Agar

A

Pseudomonas aeruginosa

59
Q

Selenite broth (Enrichment medium)

A

Salmonella spp.

60
Q

Schaedler Agar

A

Nonselective medium for aerobes and anaerobes; Selective for campylobacter and Helicobacter

61
Q

Skirrow agar

A

Campylobacter spp.

62
Q

Tetrathionate Broth

A

Salmonella and Shigella spp.

63
Q

Thayer Martin Agar

A

Neisseria gonorrhoeae and Neisseria meningitidis

64
Q

Thioglycollate

A

Aerobes and anaerobes

65
Q

Thiosulfate Citrate Bile Salt Sucrose Agar (TCBS)

66
Q

Tinsdale Agar

A

Corynebacterium diptheriae

67
Q

Todd-Hewitt Broth (selective and enrichment) With nalidixic acid and gentamicin/colistin

A

Streptococcus agalactiae in female genital specimens

68
Q

Trypticase Soy Broth (TSB)

A

Blood culture bottle base Streptococcus pneumoniae, Brucella

69
Q

Tyrosine Agar

A

Aerobic Actinomycetes

70
Q

Tween 80-Albumin Broth

A

Spirochetes

71
Q

Xylose Lysine Desoxycholate (XLD) Agar

A

Salmonella and Shigella

72
Q

Maintain the viability of organisms during specimen transport

A

Transport Media

73
Q

For Neisseria; Cary Blair-for stool pathogens like Vibrio

74
Q

Viral Transport Media

A

STUART’S

75
Q

Media for Antibiotic Susceptibility Testing

A

Mueller Hinton Agar

76
Q

Used in biochemical tests for species identification (TSI, LIA, Urea agar, SIM, Simmon Citrate agar, MRVP broth)

A

BIOCHEMICAL TEST MEDIA

77
Q

Detects organism’s motility (e.g., SIM, MIO)

A

MOTILITY MEDIUM

78
Q

Supports the growth of anaerobes (e.g., Chopped meat media, Thioglycolate)

A

ANAEROBIC MEDIA

79
Q

Transport media, maintains viability of organisms during transport

A

Amie’s Media

80
Q

Enrichment media for Vibrio

A

Alkaline Peptone water

81
Q

Selective and differential media for Bacteroides

A

Bacteroides Bile Esculin

82
Q

Differential isolation and identification of Group D Streptococci and Enterococci

A

Bile Esculin Agar

83
Q

Selective for Vancomycin resistant enterococci

A

Bile Esculin Azide agar with Vancomycin

84
Q

High protein media for growing a variety of bacteria

A

Brain Heart Infusion (BHI)

85
Q

Isolation of Salmonella typhi

A

Bismuth Sulfite Agar

86
Q

Cultivation of fastidious microorganisms and determination of haemolytic reactions

A

Blood Agar (BA)

87
Q

For Francisella tularensis

A

Blood Cystine Dextrose Agar

88
Q

Isolation of Bordetella pertussis

A

Bordet-Gengou Media

89
Q

Selective media for Salmonella (except S. typhi)

A

Brilliant Green Agar (BGA)

90
Q

Enrichment and selective for Legionella spp.

A

Buffered Charcoal Yeast Extract Agar with Antibiotics

91
Q

Selective media for Staphylococcus

A

Chapman Stone agar

92
Q

Selective for Campylobacter spp

A

Campy-Blood Agar

93
Q

Selective holding medium for recovery of Campylobacter spp

A

Campylobacter Thioglycollate Broth

94
Q

Transport media

A

Cary and Blair Media

95
Q

Used for separating Nocardia & Streptomyces

A

Casein Media

96
Q

Selective media for Campylobacter spp

A

Cefoperazone, Vancomycin, Amphotericin

97
Q

Selective for Yersinia spp.

A

Cefsulodin-Irgasan-Novobiocin

98
Q

Cultivation of Haemophilus spp. and pathogenic Neisseria

A

Chocolate Agar

99
Q

Used for isolation of anaerobes, especially Clostridium spp.

A

Chopped Meat Glucose

100
Q

Selective isolation of gram positive cocci

A

Columbia Colistin Nalidixic Acid Agar

101
Q

Used for Chlamydospore production of Candida albicans

A

Corn Meal Agar with 1% Tween 80

102
Q

Isolation of Corynebacterium diphtheriae

A

Cystine Tellurite Blood Agar

103
Q

For determining fermentation reactions of Neisseria spp.

A

Cystine Trypticase Agar

104
Q

Media for Clostridium difficile

A

Cycloserine Cefoxitin Fructose Agar

105
Q

Differentiation of lactose fermenters from non-lactose fermenters

A

Deoxycholate Agar

106
Q

For isolation of Salmonella and Shigella

A

Deoxycholate Citrate

107
Q

Selective media for Vibrios

A

Dieudonnes Media

108
Q

Isolation of Mycoplasma and Ureaplasma spp.

A

Edward Hayflick Agar

109
Q

Used for isolation and identification of Clostridium

A

Egg Yolk Agar

110
Q

Selective media for Leptospira interrogans

A

Ellinghausen, McCullough Johnson and Harris Media

111
Q

Selective media for Haemophilus ducreyi

A

Fetal Bovine Serum with Vancomycin

112
Q

For isolation of Haemophilus influenzae

A

Fildes Enrichment

113
Q

Selective enrichment for enteric pathogens

A

Gram Negative Broth

114
Q

Selective and differential for Salmonella and Shigella

A

Hektoen Enteric Agar

115
Q

Selective media for Gardnerella vaginalis

A

Human Blood Bilayer Tween

116
Q

For C. diphtheriae

A

Leoffler’s Coagulated Serum

117
Q

For isolation of Mycobacterium tuberculosis

A

Lowenstein Jensen Media

118
Q

For selection and differentiation of E. coli O157:H7

A

Mac Conkey Sorbitol Agar

119
Q

Selective for Staphylococci

A

Mannitol Salt Agar

120
Q

For isolation of Listeria monocytogenes

A

Mc Bride Media

121
Q

For isolation of Mycobacterium tuberculosis

A

Middelbrook 7H10 or 7H11

122
Q

Selective media for Borrelia species

A

Modified Kelly’s Medium

123
Q

Used in Methyl Red and Vogues Proskauer tests

A

MRVP broth

124
Q

Selective for Neisseria gonorrhoeae

A

New York City Agar

125
Q

Similar to Lowenstein Jensen, contains more malachite green

A

Petragnani’s Media

126
Q

Selective isolation of gram positive cocci and anaerobic gram negative bacilli

A

Phenylethyl Alcohol Agar

127
Q

Enrichment and selective media for Bordetella pertussis

A

Regan Lowe

128
Q

Used for fungus culture

A

Sabouraud Dextrose Maltose Agar

129
Q

Selective for Salmonella and Shigella

A

Salmonella-Shigella Agar

130
Q

Non-selective for aerobes and anaerobes

A

Schaedler Agar

131
Q

Enrichment for Salmonella spp.

A

Selenite Broth

132
Q

Test for citrate as a carbon source

A

Simmon Citrate Agar

133
Q

Selective for Campylobacter spp.

A

Skirrow Agar

134
Q

Selective for Streptococcus agalactiae

A

Streptococcal Selective Agar

135
Q

Selective for Salmonella and Shigella spp.

A

Tetrathionate Broth

136
Q

Selective for Neisseria gonorrhoeae and Neisseria meningitidis

A

Thayer Martin Agar

137
Q

Supports growth of anaerobes, aerobes, microaerophilic microorganisms

A

Thioglycollate Broth

138
Q

Selective and differential for Vibrios

A

Thiosulfate Citrate Bile Salts Agar

139
Q

Selective and enrichment for Streptococcus agalactiae

A

Todd Hewitt Broth supplemented with antibiotics

140
Q

Enrichment for subculturing bacteria

A

Trypticase Soy Broth

141
Q

Biochemical tests for differentiating enteric bacilli

A

Triple Sugar Iron Agar

142
Q

For isolation and differentiation of Salmonella and Shigella

A

Xylose Lysine Desoxycholate Agar

143
Q

Used for urease test, identifies urease producers like Proteus

BACTERIOLOGY (49 decks)

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