Biosafety and Biosecurity practices

Question 1

Prevention of bio-crime by controlling biological materials and unauthorized access, loss, theft, diversion, or intentional release. Includes password protections, access cards, inventory, infection section.

Answer 1

Biosecurity

Question 2

Principles and practices of containment to prevent unintentional exposure to pathogens and toxins or their unintentional release. Protects people from germs using BSC and monitoring.

Answer 2

Biosafety

Question 3

Personnel security measures including access controls, physical security (CCTV, password-protected doors), material control, quarantine, and policies.

Answer 3

Biosecurity

Question 4

Use of basic PPE, including donning and doffing procedures, spill kits, engineering controls such as BSC and fume hoods, and sterilization, disinfection, and isolation techniques.

Answer 4

Biosafety

Question 5

Donning PPE order: Gown, Mask, Goggles, Gloves.

Answer 5

PPE Procedure

Question 6

Doffing PPE order: Gloves, Goggles, Gown, Mask.

Answer 6

PPE Procedure

Question 7

Alternative doffing PPE order: Gown & Gloves, Goggles, Mask.

Answer 7

PPE Procedure

Question 8

Laboratory Response Network (LRN) highest rank; responsible for definitive characterization of pathogens (e.g., CDC).

Answer 8

National Laboratories

Question 9

Laboratory Response Network (LRN) second rank; performs confirmatory testing of pathogens using specific reagents and technology (e.g., RITM).

Answer 9

Reference Laboratories

Question 10

Laboratory Response Network (LRN) lowest rank; detects and recognizes possible bioterrorism events and conducts surveillance.

Answer 10

Sentinel Laboratories

Question 11

Universal precaution for treating human blood and body fluids as infectious for blood-borne pathogens (e.g., HIV, HBV).

Answer 11

Universal Precaution (UP)

Question 12

Modification of UP to treat all body substances as infectious. Hand washing not recommended after removing gloves if hands are not visibly soiled.

Answer 12

Body Substance Isolation (BSI)

Question 13

Applies only to blood and all body fluids/secretions except sweat, non-intact skin, and mucous membranes.

Answer 13

Standard Precaution

Question 14

Category of Bioterrorism Agents; highest risk, easily transmitted, highly infectious (e.g., smallpox, anthrax, Y. pestis).

Answer 14

Category A

Question 15

Category of Bioterrorism Agents; moderately easy to transmit, moderate to low mortality (e.g., Brucella, C. perfringens toxins).

Answer 15

Category B

Question 16

Category of Bioterrorism Agents; emerging/recurrent, could be engineered in the future (e.g., Ebola, MERS-CoV, Hemorrhagic fever viruses).

Answer 16

Category C

Question 17

Donning procedure

Answer 17

removal of jewelry, inspection of PPE, handwashing.

Question 18

Donning order

Answer 18

Shoe/Boot cover > Inner gloves > Coverall/Gown > N95 Respirator > Surgical hood/Head cover > Outer Apron > Outer gloves > Face shield/Goggles.

Question 19

Final donning step

Answer 19

Verify mobility, final check by spotter/observer.

Question 20

Doffing procedure

Answer 20

inspect PPE for damage after working, disinfect with 70% alcohol or 10% bleach.

Question 21

Doffing order

Answer 21

Outer Apron > Outer gloves > Face shield/Goggles > Surgical hood/Head cover > Coverall/Gown > Shoe/Boot cover > (Change Inner gloves) > N95 Respirator.

Question 22

After doffing, perform

Answer 22

handwash.

Question 23

N95 respirators must be

Answer 23

fit-tested and should have a snug fit. Men should shave their beards.

Question 24

Disinfect gloves between uses with

Answer 24

70% alcohol or 10% bleach.