Microscopy Flashcards
Brain protected by the
meninges and skull
meninges (dura) are thicker..
inbetween the hemispheres and the cerebellum/cortex area
2 main staining methods and where do you use them for?
- histochemistry
- immunohistochemistry
you use them for determining the location of a protein
histochemistry basically
uses dye to stain chromogens
immunohistochemistry
uses antibody to bind to chromogens/flurogens
3 typen histochemistry
- silver staining
- cell staining
- lipid staining LFB
cell staining
- nissl staining -> stains all neurons and glial cells through RNA
- DAPI staining -> stains all cell nuclei through DNA
lipid staining LFP
used to stain myelin! therefore good to see white matter lesions in MS
immunohistochemistry basic
antibodies that bind to proteins -> you can visualize where the proteins are at that time.
protein - antibody - color
use of chromogens
Kleurloze chemische verbinding die door een reactie kleur kan gaan geven
Cheap reliable fast used for light microscopy can be combined
fluophore
expensive
high quality
combined
lastig om right fluophores te kiezen
wat kan je met fluorophores goed doen?
do co-localization van 2 verschillende proteins zien binnen een cel. wide field en confocal.
hallmark of MS
demyelination of axons, followed by degeneration of those axons
welke staining gebruiken bij MS?
PLP staining, of LFP staining
active, chronic active and inactive lesion
high inflammatory activity inside lesion
high inflammatory activity in the rim around the lesion
no inflammatory activity, gliosis
