Lecture 8 - Effector T Cell Response Flashcards

1
Q

What changes are seen in naive T cell priming

A

1 - forms a stable synapse
2 - increase T cell adhesiveness to APCs
3 - induction of pro-survival signal
4 - increase in metabolic activity
5 - increased transcription

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2
Q

why is it necessary to increase the metabolic activity of T cells

A

for cell proliferation and production of effector molecules, receptors, integrins, etc.

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3
Q

How is TCR signaling initiated

A

tyrosine phosphorylation within the cytoplasmic region of immunoreceptors (ITAMs)

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4
Q

summarize:

PLC-y-mediated T-cell activation

A
  1. intracellular calcium entry
  2. activation of RAS
  3. activation of protein kinase
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5
Q

summarize:

T cell changes after TCR signaling

A
  1. increased cell survival
  2. increased metabolic activity
  3. increased gene transcription
  4. increased growth factor production
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6
Q

The T cell receptor has many functional components, some of which are involved in signal transduction, while others are involved in antigen recognition. What two elements are involved in antigen recognition?

A

TCRa and TCRb

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7
Q

Why are lymph nodes palpable after infection

A

rapid stromal expansion of immune response

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8
Q

What antigen receptors give rise to CD4 an CD8 T cells

A

alpha and beta

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9
Q

T/F: cytokines in APCs dictate what T cell differentiate to

A

True

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10
Q

what are the master regulator transcription factors of CD4+ T cells

A

T-bet
GATA-4
RORyT
BcI-6
FoxP3

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11
Q

T/F: T helper cells regulate

A

False - activate

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12
Q

What T helper cell targets extracellular bacteria

A

TH17

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13
Q

what immune cells do TH17 target for enhanced response

A

neutrophils

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14
Q

What major cytokines and their actions in TH17

A

IL17 and IL22

recruit neutrophils
increase cell turnover
direct killing of bacterium on epithelium

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15
Q

What T helper cell targets intracellular bacteria

A

TH1

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16
Q

what immune cells do the TH1 target for enhanced response

A

macrophage

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17
Q

What major cytokines and their actions have in TH1

A

IFN-y

recruit macrophages and increase phagocytotic capability
kills chronically infected cells

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18
Q

What immune cell targets helminth infections

A

TH2

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19
Q

what immune cells do the TH2 target for enhanced response

A

eosinophils, mast cells, basophils

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20
Q

What major cytokines and their actions have in TH2

A

IL4/5

increased cell turnover and mucus
increase smooth muscle contraction

21
Q

T/F: antibodies are effective no matter where they are found

A

False - only effective while a pathogen is outside the cell

22
Q

How are cytotoxic T cells like serial killers

A

recognizes “targets” (viral-infected cell), will bind and initiate apoptosis on specific cell

23
Q

extrinsic removal

A

activation of death receptors via ligands

24
Q

intrinsic or mitochondrial removal

A

response to noxious stimuli

25
Q

what specialized protease is common to both extrinsic/intrinsic pathways

A

aspartic acid-specific cysteine proteases (CASPASES)

26
Q

Initiator caspases

A

promote apoptosis by cleaving and activating others

27
Q

Effector caspases

A

initiate intracellular breakdown for apoptosis

28
Q

Perforin

A

delivers contents of granules into the target cell cytoplasm

29
Q

Granzymes

A

activate apoptosis in the cytoplasm of the target cell

30
Q

Granulysin

A

antimicrobial actions

31
Q

summarize what happens once a cytotoxic T-cell

A

1 - perforin creates pores
2 - granzymes enter the cell and trigger mitochondria
3 - cytochrome C released
4 - caspase activation
5 - intracellular changes
6 - apoptosis

32
Q

What are the 4 steps in T cell killing of target cells

A

1 - recognition
2 - proliferation
3 - differentiation
4 - effector (killing)

33
Q

Define what co-inhibitory receptors do

A

regulate un-controlled activation of lymphocytes through competition with CD28

34
Q

What are the immune checkpoints

A

co-inhibitory molecules expressed by T cells CD152 and PD1

35
Q

ITIM

A

immunoreceptor tyrosine-based inhibitory motif

36
Q

ITIM inhibits TCR signaling through

A

dephosphorylation

37
Q

How do Tregs regulate T effector cells

A

directly or via APCs

38
Q

how does infection response progress in the body

A
  1. establishment of infection
  2. inductive phase
  3. effector phase
  4. memory phase
39
Q

What does the asymmetric division of a T cell produce

A

Helper and Memory T cell precursors

40
Q

how do corticosteroids work

A

block NF-kB responses (T cell proliferation, apoptosis of developing thymocytes, etc)

41
Q

how does tacrolimus work

A

blocks IL, IFN-y

42
Q

How does cyclosporine work

A

selective immunosuppressant blocks TH1 responses

43
Q

What 3 signals do APCs deliver to T cells

A
  1. TCR activation
  2. Survival
  3. Differentiation
44
Q

Naive CD4+ cells differentiate to

A

TH1, TH2, TH17, THf, THreg

45
Q

TH1 secretes _____ and provides immunity against ______ bacteria

A

IFN-y and IL-2; intracellular

46
Q

TH2 secretes ______ and provides immunity against ______ bacteria

A

IL 4/5/13; helminths

47
Q

TH17 secretes ______ and provides immunity against ______ bacteria

A

IL17/22; extracellular

48
Q

THf secretes _____ and

A

IL21; help b cells isotope switching