Lecture 32 - Bacteriology Lab Flashcards
what are the advantages and disadvantages of:
acetate tape
advantage: good for dry lesions, immediate results, cheap
disadvantage: commensal bacteria and yeast, easily contaminated
what are the advantages and disadvantages of:
impression smear
advantage: good for moist lesions, immediate results, cheap
disadvantage: commensal bacteria
what are the advantages and disadvantages of:
pustule cytology
advantage: direct sampling, avoids skin surface contaminants, cheap
disadvantage: invasive, only intact pustules
what are the advantages and disadvantages of:
superficial scrape
advantage: good for greasy debris from the skin surface
disadvantage: more invasive, not for dry lesions
what are the advantages and disadvantages of:
culture
advantage: direct ID, antimicrobial susceptibility report
disadvantage: expensive, delayed results
what are the advantages and disadvantages of:
punch biopsy
advantage: can see infectious organisms within the structure of the skin
disadvantage: more invasive, delayed results, expensive
what are the advantages and disadvantages of:
free catch
advantage: quick, non-invasive
disadvantage: contamination!!!
what are the advantages and disadvantages of:
cystocentesis
advantage: quick, minimal risk of contamination
disadvantage: risk of damage to the bladder, moderately invasive
what are the advantages and disadvantages of:
catheterized
advantage: limited risk of trauma, can collect mucosal lining
disadvantage: introduces bacteria, difficult
what are the advantages and disadvantages of:
deep nasal swab
advantage: least invasive
disadvantage: contamination, commensal bacteria
what are the advantages and disadvantages of:
transtracheal wash
advantage: more accurate for lower airway
disadvantage: sedation required
what are the advantages and disadvantages of:
bronchoalveolar lavage
advantage: more accurate for lower airway
disadvantage: sedation required
what are the advantages and disadvantages of:
respiratory cytology/PCR
advantage: help w pathogen identification
disadvantage: can be expensive
gram-positive cell wall structure
thick peptidoglycan
periplasmic space
plasma membrane
gram-negative cell wall structure
outer membrane
thin peptidoglycan
inner membrane