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Animal Health & Disease 1 > Lecture 32 - Bacteriology Lab > Flashcards

Lecture 32 - Bacteriology Lab Flashcards

1
Q

what are the advantages and disadvantages of:

acetate tape

A

advantage: good for dry lesions, immediate results, cheap

disadvantage: commensal bacteria and yeast, easily contaminated

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2
Q

what are the advantages and disadvantages of:

impression smear

A

advantage: good for moist lesions, immediate results, cheap

disadvantage: commensal bacteria

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3
Q

what are the advantages and disadvantages of:

pustule cytology

A

advantage: direct sampling, avoids skin surface contaminants, cheap

disadvantage: invasive, only intact pustules

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4
Q

what are the advantages and disadvantages of:

superficial scrape

A

advantage: good for greasy debris from the skin surface

disadvantage: more invasive, not for dry lesions

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5
Q

what are the advantages and disadvantages of:

culture

A

advantage: direct ID, antimicrobial susceptibility report

disadvantage: expensive, delayed results

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6
Q

what are the advantages and disadvantages of:

punch biopsy

A

advantage: can see infectious organisms within the structure of the skin

disadvantage: more invasive, delayed results, expensive

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7
Q

what are the advantages and disadvantages of:

free catch

A

advantage: quick, non-invasive

disadvantage: contamination!!!

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8
Q

what are the advantages and disadvantages of:

cystocentesis

A

advantage: quick, minimal risk of contamination

disadvantage: risk of damage to the bladder, moderately invasive

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9
Q

what are the advantages and disadvantages of:

catheterized

A

advantage: limited risk of trauma, can collect mucosal lining

disadvantage: introduces bacteria, difficult

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9
Q

what are the advantages and disadvantages of:

deep nasal swab

A

advantage: least invasive

disadvantage: contamination, commensal bacteria

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10
Q

what are the advantages and disadvantages of:

transtracheal wash

A

advantage: more accurate for lower airway

disadvantage: sedation required

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11
Q

what are the advantages and disadvantages of:

bronchoalveolar lavage

A

advantage: more accurate for lower airway

disadvantage: sedation required

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12
Q

what are the advantages and disadvantages of:

respiratory cytology/PCR

A

advantage: help w pathogen identification

disadvantage: can be expensive

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13
Q

gram-positive cell wall structure

A

thick peptidoglycan
periplasmic space
plasma membrane

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14
Q

gram-negative cell wall structure

A

outer membrane
thin peptidoglycan
inner membrane

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15
Q

gram + appears as ____ after a stain and gram - appears as ____

A

purple; red

16
Q

acid-fast + appears as ____ after a stain and acid-fast - appears as ____

A

pink; purple

Animal Health & Disease 1 (52 decks)

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