ID 1 Flashcards

1
Q

Flagellum composition

A

proteins

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2
Q

pilus/fimbriae composition

A

glycoprotein

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3
Q

Which type of bacteria form spores?

A

Only gram positives.

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4
Q

spore structure

A

1) keratin-like coat
2) *dipicolinic acid
3) peptidoglycan, DNA

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5
Q

B anthraces capsule

A

Unique in containing poly-D glutamate

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6
Q

Glycocalyx composition?

A

Loose network of polysaccharides.

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7
Q

glycocalyx function

A

Mediates adherence to surfaces, especially foreign surfaces (eg, indwelling catheters)

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8
Q

Which types of bacteria contain an outer membrane?

A

Gram negative only

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9
Q

porin function?

A

transport across outer membrane

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10
Q

Outer membrane composition?

A

Outer leaflet: contains endotoxin (LPS/LOS)
Embedded proteins: porins and other outer membrane proteins (OMPs)
*most OMPs are antigenic
Inner leaflet: phospholipids

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11
Q

What bacteria have a periplasm?

A

gram-negatives

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12
Q

What is the periplasm?

A

Space between cytoplasmic membrane and outer membrane (peptidoglycan in middle)

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13
Q

caveat about periplasm

A

Accumulates components exiting gram negative cells, including hydrolytic enzymes (eg, beta-lactamases)

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14
Q

cell wall composition

A

peptidoglycan sugar backbone with peptide side chains cross-linked by transpeptidase.

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15
Q

Where are penicillin binding proteins?

A

cytoplasmic membrane

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16
Q

bacillus structure

A

gram positive rod

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17
Q

RNA sense in viruses concept

A

/positive sense = viral RNA sequence can be directly translated into viral proteins. Viral RNA can be considered mRNA. ***Thus, these viruses do not need an RNA polymerase packaged into the virion. They hook up to ribosomes, and the reading frame is translated. The ribosome makes a polymerase for them. /negative sense = complementary to viral mRNA, thus positive-sense RNA must be produced by an RNA-dependent RNA polymerase prior to translation. These viruses have polymerases in their capsules for this purpose.

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18
Q

Memorize gram positive structure vs. gram negative

A

FA 108

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19
Q

bacillus structure

A

gram positive rod

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20
Q

gardnerella structure

A

gram positive rod

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21
Q

lactobacillus structure

A

gram positive rod

22
Q

all mycobacteria are..

A

gram positive rod

23
Q

propionibacterium are

A

gram positive rod

24
Q

campylobacter

A

gram negative rod

25
enterobacter
gram negative rod
26
helicobacter
gram negative rod
27
klebsiella
gram negative rod
28
proteus
gram negative rod
29
pseudomonas
gram negative rod
30
salmonella
gram negative rod
31
serratia
gram negative rod
32
shigella
gram negative rod
33
vibrio
gram negative rod
34
yersinia structure
gram negative rod
35
bordetella
gram negative rod
36
burkholderia
gram negative rod
37
what system does burkholderia affect?
respiratory pathogen
38
legionella
gram negative rod
39
bartonella
gram negative rod
40
brucella
gram negative rod
41
francisella
gram negative rod
42
pasteurella
gram negative rod
43
pleomorphic bacteria
1) chlamydiae | 2) rickettsiae
44
chlamydia stain?
giemsa
45
borrelia stain
giemsa
46
bacteria without cell walls
1) mycoplasma | 2) ureaplasma
47
Bacteria you can't gram stain? why?
mycoplasma or ureaplasma since cell walls contain sterols rather than peptidoglycan, which don't stain
48
what do gram positives retain with staining?
crystal violet
49
bugs that don't stain well...
``` These Microbes May Lack Real Color Treponema, leptospira Mycobacteria Mycoplasma, Ureaplasma Legionella Rickettsia Chlamydia Bartonella Ehrlichia Anaplasma ```
50
Why can't you stain spirochetes?
too thin