Biochem II

Question 1

What is the function of the Cahill cycle?

Answer 1

It’s the alanine cycle. Think of alan on escalator code in front of his yard. Series of reactions in which amino groups and carbons from muscle are transported to the liver.

Question 2

Function of Cori cycle?

Answer 2

Lactate generated from skeletal muscle metabolism is transported back to the liver to be converted to pyruvate and then to glucose.

Question 3

Draw out structure of urea

Answer 3

FA 93

Question 4

Site of action of alcohol dehydrogenase?

Answer 4

cytosol

Question 5

Site of action of acetaldehyde dehydrogenase?

Answer 5

mitochondria

Question 6

Southern blotting procedure

Answer 6

1. DNA sample enzymatically cleaved into smaller pieces, which are separated on a gel by electrophoresis, and then transferred to a filter.
2. Filter is exposed to radio labeled DNA probe that recognizes and anneals to its complementary strand.
3. Resulting doble-stranded, labeled piece of DNA is visualized when filter is exposed to the film.