GRAM NEGATIVE RODS - Respiratory Tract Flashcards
enriched chocolate agar
POLYRIBITOL PHOSPHATE CAPSULE
Haemophilus influenzae type B
Bordet-Gengou Agar
Whooping cough
Bordetella pertussis
Poorly gram staining
silver stain
charcoal yeast agar
airconditioning
Legionella pneumophila
Requires factor X (hemin) and V (NAD) for growth
Haemophilus influenzae
Lab diagnosis for Haemophilus influenzae
Chocolate agar
-culture specimen on blood agar that has been heated to 80 degree for 15 mins
Lab diagnosis for Bordetella pertussis
Regan Lowe Charcoal medium
Natural History of Bordetella Infection
Incubation Period
Catarrhal Phase
Paroxysmal Phase
Convalescent Stage
1-2 weeks
rhinorrhea, malaise, fever, sneezing, anorexia
ANTIBIOTICS MOST EFFECTIVE
Catarrhal Phase
-most infectious phase
2-4 weeks
whoop – burst of non productive coughs
increased number of lymphocytes in blood smear - LEUKEMOID REACTION
antibiotics INEFFECTIVE
Paroxysmal Phase
3-4 weeks (or longer)
diminished paroxysmal cough
development of secondary complications
Convalescent Stage
COMPLICATIONS
- pneumonia
- seizure
- encephalopathy
Stain used for Legionella
Dieterle’s silver stain
Legionella is grown on
Buffered Charcoal Yeast Extract Agar
- L-cysteine
- Iron
mild, flulike illness
Pontiac fever
pneumonia, cough, fever
confusion, non-bloody diarrhea, HYPONATREMIA, proteinuria and hematuria
Legionnaire’s disease
23/M, fishpond cleaner developed fever, cough and body weakness. If you are suspecting Pontiac fever because of the occupational history, which of the ff CXR findings would you expect?
NEGATIVE SIGN
