DNA extraction - SB3

Question 1

what equipment is needed to extract DNA?

Answer 1

3g salt
100cm3 of water
beaker
10cm3 washing up liquid
50g of peas
water bath (60 degrees)
filter paper
funnel
flask
boiling tube
2 drops protease enzyme solution
ethanol (cold)
glass stirring rod

Question 2

what is the method for extracting DNA?

Answer 2

1. dissolve salt in beaker filled with water - add washing up liquid and stir till salt is dissolved
2. Mash peas - put peas into an empty beaker and add solution from 1 - stir slowly
3. place beaker in water bath for 15 minutes
4. Filter mixture and collect filtrate in beaker
5. pour into boiling tube and add protease enzyme solution
6. slowly pour in cold ethanol onto the side of the tube. equal amounts ethanol to filtrate
7. Sit for few mins while precipitate forms between ethanol and filtrate
8. DNA can be collected with stir rod

Question 3

why is DNA extraction used?

Answer 3

to identify origins of food and bases within

Question 4

why is washing up liquid important to the experiment?

Answer 4

Breaks down cell surface membranes and membranes around the nuclei

Question 5

why is ethanol used?

Answer 5

insoluble to DNA so guarantees precipitate is formed