[38] Cryo-Electron Microscopy Flashcards
What is Cryo-Electron Microscopy (Cryo-EM)?
A type of electron microscopy where the sample is studied at cryogenic temperatures.
Cryo-EM - Initials
Cryo-Electron Microscopy
What is the main advantage of Cryo-EM?
It allows the observation of specimens that have not been stained or fixed in any way, showing them in their native environment.
What is a significant feature of Cryo-EM in the field of structural biology?
It enables the high-resolution structural determination of biomolecules.
How is the sample prepared in Cryo-EM?
The sample is rapidly frozen to prevent the formation of ice crystals.
What are some applications of Cryo-EM?
Study of cellular structures, viruses, and protein complexes.
What type of imaging does Cryo-EM produce?
It provides three-dimensional imaging.
How does Cryo-EM contribute to drug discovery?
By revealing the structures of proteins at atomic resolution, it can guide drug design.
What is the difference between Cryo-EM and traditional electron microscopy?
Cryo-EM examines samples at cryogenic temperatures, preserving their natural structure, while traditional electron microscopy may require staining and other preparation methods that can alter the sample.
What is Cryo-Electron Tomography (Cryo-ET)?
A type of Cryo-EM that collects images from different angles to create a 3D reconstruction.
Cryo-ET - Initials
Cryo-Electron Tomography
What kind of resolution can Cryo-EM achieve?
Near-atomic resolution.
How has Cryo-EM impacted the study of viruses?
It has enabled detailed structural study of viruses in near-native states.
How does Cryo-EM maintain sample hydration?
By rapid freezing that preserves water in its vitreous (glass-like) state.
What are two main types of Cryo-EM?
Single-particle analysis (SPA) and Cryo-electron tomography (Cryo-ET).
