[13] DNA Footprinting and DNase-Seq Flashcards
What is DNA Footprinting?
A technique for identifying DNA sequences that protein molecules bind with.
What is DNase-Seq?
A method for identifying DNase I hypersensitive sites, which are regions of the genome of particular importance in gene regulation.
What is the application of DNA Footprinting and DNase-Seq?
Both techniques provide insights into gene regulation.
What is the process of DNA Footprinting?
- Binding of a protein to a DNA sequence
- Treatment with a DNA-cleaving chemical
- Unbound regions are cleaved, bound regions are protected.
What is the process of DNase-Seq?
- Treatment of genomic DNA with DNase I
- Sequencing of the resulting small fragments.
Why are DNA Footprinting and DNase-Seq important?
They aid in understanding protein-DNA interactions and how these interactions influence gene expression.
What are the limitations of DNA Footprinting and DNase-Seq?
- Requires high-quality DNA
- Requires high specificity of the protein of interest.
How do DNA Footprinting and DNase-Seq contribute to the understanding of gene regulation?
By identifying DNA sequences that proteins bind with (Footprinting) and regions of the genome that are hypersensitive to DNase I (DNase-Seq), these techniques help reveal how gene expression is controlled.
Why does DNA Footprinting require high-quality DNA?
High-quality DNA is needed to ensure accurate and reliable results when identifying DNA sequences that protein molecules bind with.
Why does DNase-Seq require high specificity of the protein of interest?
The specificity of the protein of interest is important as it helps in accurately identifying DNase I hypersensitive sites.
