[22] Flow Cytometry

Question 1

What is Flow Cytometry?

Answer 1

A technology that simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light.

Question 2

Define the initials FC

Answer 2

Flow Cytometry

Question 3

What are the main applications of Flow Cytometry?

Answer 3

• Cell counting
• Cell sorting
• Biomarker detection
• Protein engineering
• Cell cycle analysis
• Apoptosis

Question 4

What is cell counting in the context of Flow Cytometry?

Answer 4

A measure of the concentration of cells in a sample.

Question 5

What is cell sorting in the context of Flow Cytometry?

Answer 5

The process of separating cells based on their properties.

Question 6

What is biomarker detection in the context of Flow Cytometry?

Answer 6

The identification and quantification of specific cellular markers.

Question 7

What is protein engineering in the context of Flow Cytometry?

Answer 7

The use of Flow Cytometry to investigate the expression and function of engineered proteins.

Question 8

What is cell cycle analysis in the context of Flow Cytometry?

Answer 8

The use of Flow Cytometry to identify cells in different phases of the cell cycle.

Question 9

What is apoptosis in the context of Flow Cytometry?

Answer 9

The use of Flow Cytometry to detect and measure programmed cell death.

Question 10

How does Flow Cytometry work?

Answer 10

Cells are stained with specific fluorescent dyes, then passed through a laser light beam, where they emit light at different wavelengths. These signals are collected and analyzed.

Question 11

What are the components of a Flow Cytometer?

Answer 11

• Fluidics system
• Optics system
• Detection and electronic system

Question 12

What is the role of fluidics system in Flow Cytometry?

Answer 12

It controls the movement of cells in a stream of fluid.

Question 13

What is the role of optics system in Flow Cytometry?

Answer 13

It is responsible for the generation of light and collection of emitted light.

Question 14

What is the role of detection and electronic system in Flow Cytometry?

Answer 14

It translates the detected light signals into electronic signals which can be processed and analyzed.

Question 15

What is a histogram in Flow Cytometry?

Answer 15

A graphical representation of the distribution of detected cells based on a single parameter.

Question 16

What is a dot plot in Flow Cytometry?

Answer 16

A graphical representation of two parameters measured in a cell population.

Question 17

What is the difference between forward scatter (FSC) and side scatter (SSC) in Flow Cytometry?

Answer 17

FSC is proportional to cell size or volume, SSC provides information about cell complexity or granularity.

Question 18

What are the advantages of Flow Cytometry?

Answer 18

• Rapid analysis
• Ability to analyze multiple parameters simultaneously
• High sensitivity and specificity

Question 19

What are the limitations of Flow Cytometry?

Answer 19

• Requires skilled personnel
• High initial cost
• Limited by the number of fluorochromes that can be distinguished simultaneously

Question 20

How has Flow Cytometry revolutionized immunology?

Answer 20

It allows for precise identification and quantification of different immune cell types and their functions.