[20] Prime Editing Flashcards
What is Prime Editing?
A versatile and precise genome editing method that directly writes new genetic information into a specified DNA site.
What method does Prime Editing use?
It uses a catalytically impaired Cas9 fused to an engineered reverse transcriptase and a prime editing guide RNA (pegRNA).
How does the process of Prime Editing work?
The pegRNA both specifies the target site and encodes the desired edit, while the engineered reverse transcriptase and impaired Cas9 mediate the writing of new information.
What are the applications of Prime Editing?
Prime editing has a wide range of applications in biomedical research, medicine, and biotechnology.
What is the advantage of Prime Editing?
This method can introduce a broad range of edits, including insertions, deletions, and all 12 possible base-to-base conversions, with fewer byproducts compared to other methods.
What is the role of pegRNA in Prime Editing?
The pegRNA both specifies the DNA target site and encodes the desired DNA edit in Prime Editing.
How does Prime Editing differ from other genome editing methods?
Prime Editing can introduce a broader range of edits with fewer byproducts compared to other methods.
What is the role of the impaired Cas9 and the engineered reverse transcriptase in Prime Editing?
These components mediate the writing of new genetic information into a specified DNA site.
What kinds of edits can Prime Editing introduce?
Prime Editing can introduce insertions, deletions, and all 12 possible base-to-base conversions.
