[03] Long-read Sequencing Technologies (PacBio, Nanopore)

Question 1

What is the abbreviation for Pacific Biosciences?

Answer 1

PacBio

Question 2

What does PacBio stand for?

Answer 2

Pacific Biosciences

Question 3

What are some long-read sequencing technologies?

Answer 3

• Pacific Biosciences (PacBio)
• Oxford Nanopore Technologies

Question 4

How does PacBio sequencing work?

Answer 4

It uses single molecule, real-time (SMRT) sequencing to determine the sequence of nucleotides in a single DNA molecule.

Question 5

What is the abbreviation for Oxford Nanopore Technologies?

Answer 5

ONT

Question 6

What does ONT stand for?

Answer 6

Oxford Nanopore Technologies

Question 7

How does Nanopore sequencing work?

Answer 7

It involves passing a single DNA molecule through a nanopore and measuring changes in electrical conductivity to determine the DNA sequence.

Question 8

What are the advantages of long-read sequencing technologies like PacBio and Nanopore?

Answer 8

• Sequencing of longer reads
• Identification of large structural variations
• Sequencing through inaccessible regions of the genome with short-read technologies

Question 9

What are the disadvantages of long-read sequencing technologies like PacBio and Nanopore?

Answer 9

• Higher error rates compared to short-read sequencing technologies
• More expensive

Question 10

What is the abbreviation for Single Molecule, Real-Time sequencing?

Answer 10

SMRT

Question 11

What does SMRT stand for?

Answer 11

Single Molecule, Real-Time sequencing

Question 12

What is the key feature of long-read sequencing technologies?

Answer 12

They provide longer read lengths compared to short-read sequencing.

Question 13

How do long-read sequencing technologies improve genome assembly?

Answer 13

They can sequence longer reads, which simplifies the assembly process.

Question 14

Why can long-read sequencing technologies identify large structural variations?

Answer 14

Because they can read longer sequences, they are able to detect larger changes in the structure of the DNA.

Question 15

How can long-read sequencing technologies access regions of the genome inaccessible to short-read technologies?

Answer 15

Their longer read lengths allow them to sequence through regions that are too complex or repetitive for short-read technologies.

Question 16

Why do long-read sequencing technologies have higher error rates compared to short-read sequencing technologies?

Answer 16

The techniques used in long-read sequencing, like single molecule sequencing, are less accurate.

Question 17

Why are long-read sequencing technologies more expensive?

Answer 17

The equipment and reagents required for these technologies are costlier.