[02] Single-cell RNA Sequencing

Question 1

What is the abbreviation for Single-cell RNA sequencing?

Answer 1

scRNA-seq

Question 2

What does scRNA-seq stand for?

Answer 2

Single-cell RNA sequencing

Question 3

What technique is used in scRNA-seq?

Answer 3

Next-generation sequencing (NGS)

Question 4

What is the purpose of scRNA-seq?

Answer 4

It provides a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment.

Question 5

Can you describe the process of scRNA-seq?

Answer 5

1. Isolation of cells of interest
2. Conversion of RNA into cDNA
3. Amplification and sequencing of cDNA
4. Mapping reads back to the reference genome

Question 6

Where is scRNA-seq mainly applied?

Answer 6

• Cancer research
• Developmental biology
• Immunology
• Neurobiology

Question 7

What are the advantages of scRNA-seq?

Answer 7

• Uncovering cellular heterogeneity
• Identification of novel cell types
• Tracking the developmental lineage of cells

Question 8

What are the limitations of scRNA-seq?

Answer 8

• Expensive
• Technically challenging
• Noisy and sparse data

Question 9

What is the abbreviation for Next-Generation Sequencing?

Answer 9

NGS

Question 10

What does NGS stand for?

Answer 10

Next-Generation Sequencing

Question 11

How does scRNA-seq contribute to cancer research?

Answer 11

It identifies different cell types within a tumor.

Question 12

How does scRNA-seq contribute to developmental biology?

Answer 12

It traces lineage decisions.

Question 13

How does scRNA-seq contribute to immunology?

Answer 13

It characterizes immune cells.

Question 14

How does scRNA-seq contribute to neurobiology?

Answer 14

It classifies neurons.

Question 15

How is RNA converted into cDNA in scRNA-seq?

Answer 15

Through a process known as reverse transcription.

Question 16

What happens to the cDNA after its creation in scRNA-seq?

Answer 16

It is amplified and sequenced.

Question 17

What is done with the resulting reads from the sequencing in scRNA-seq?

Answer 17

They are mapped back to the reference genome.

Question 18

Why is the data from scRNA-seq often noisy and sparse?

Answer 18

It captures only a small proportion of the total transcripts in a cell.

Question 19

What makes scRNA-seq a technically challenging process?

Answer 19

The process of isolating individual cells and ensuring the RNA is adequately converted, amplified, and sequenced is complex.