[02] Single-cell RNA Sequencing Flashcards
What is the abbreviation for Single-cell RNA sequencing?
scRNA-seq
What does scRNA-seq stand for?
Single-cell RNA sequencing
What technique is used in scRNA-seq?
Next-generation sequencing (NGS)
What is the purpose of scRNA-seq?
It provides a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment.
Can you describe the process of scRNA-seq?
- Isolation of cells of interest
- Conversion of RNA into cDNA
- Amplification and sequencing of cDNA
- Mapping reads back to the reference genome
Where is scRNA-seq mainly applied?
- Cancer research
- Developmental biology
- Immunology
- Neurobiology
What are the advantages of scRNA-seq?
- Uncovering cellular heterogeneity
- Identification of novel cell types
- Tracking the developmental lineage of cells
What are the limitations of scRNA-seq?
- Expensive
- Technically challenging
- Noisy and sparse data
What is the abbreviation for Next-Generation Sequencing?
NGS
What does NGS stand for?
Next-Generation Sequencing
How does scRNA-seq contribute to cancer research?
It identifies different cell types within a tumor.
How does scRNA-seq contribute to developmental biology?
It traces lineage decisions.
How does scRNA-seq contribute to immunology?
It characterizes immune cells.
How does scRNA-seq contribute to neurobiology?
It classifies neurons.
How is RNA converted into cDNA in scRNA-seq?
Through a process known as reverse transcription.
What happens to the cDNA after its creation in scRNA-seq?
It is amplified and sequenced.
What is done with the resulting reads from the sequencing in scRNA-seq?
They are mapped back to the reference genome.
Why is the data from scRNA-seq often noisy and sparse?
It captures only a small proportion of the total transcripts in a cell.
What makes scRNA-seq a technically challenging process?
The process of isolating individual cells and ensuring the RNA is adequately converted, amplified, and sequenced is complex.
