[18] Next Generation Sequencing (NGS) Technologies Flashcards
What is NGS?
High-throughput technology that allows for sequencing of DNA and RNA much more quickly and cheaply than traditional sequencing methods.
Define NGS initials
Next Generation Sequencing
What are the main applications of NGS?
- Whole genome sequencing
- Targeted gene sequencing
- Metagenomics
- Genotyping
- RNA sequencing
What is whole genome sequencing in the context of NGS?
A comprehensive method for analyzing entire genomes.
What is targeted gene sequencing in the context of NGS?
The study of specific genes or regions of interest within a genome.
What is metagenomics in the context of NGS?
The study of genetic material directly from environmental samples.
What is genotyping in the context of NGS?
The process of determining differences in the genetic make-up of an individual by examining the individual’s DNA sequence.
What is RNA sequencing in the context of NGS?
The use of NGS to reveal the presence and quantity of RNA in a biological sample at a given moment.
What are the key advantages of NGS over traditional sequencing methods?
- Higher throughput
- Lower cost
- Greater speed
- More detailed information
What are some limitations of NGS?
- Requires high computational resources
- Data storage and management challenges
- Errors in sequencing
What is Illumina sequencing?
A commonly used NGS technology that uses sequencing by synthesis method.
What is Ion Torrent sequencing?
An NGS technology that detects hydrogen ions released during DNA replication.
What is Pyrosequencing?
An NGS method that uses enzyme cascade and light detection to sequence DNA.
What is Oxford Nanopore sequencing?
A sequencing technology that reads DNA strands directly as they pass through a nanopore.
How has NGS impacted genomics research?
It has revolutionized genomics by allowing for rapid sequencing of large portions of the genome at a relatively low cost.