[12] Phage Display and Antibody Libraries

Question 1

What is phage display?

Answer 1

A technique used to study protein-protein, protein-peptide, and protein-DNA interactions.

Question 2

What are bacteriophages?

Answer 2

Viruses that infect bacteria.

Question 3

Initials: M13

Answer 3

A filamentous bacteriophage used in phage display

Question 4

What is an antibody library?

Answer 4

A collection of diverse antibodies (or fragments) expressed in a host, such as bacteria or yeast, for selection of binders to a target antigen.

Question 5

What is the connection between phage display and antibody libraries?

Answer 5

Phage display is a method used to create and screen antibody libraries for the identification of novel antibody fragments.

Question 6

What is a filamentous bacteriophage?

Answer 6

A type of bacteriophage, such as M13, that infects bacteria and is used in phage display technology.

Question 7

What is the biopanning process?

Answer 7

A technique used in phage display to select for phages expressing specific desired peptides or proteins.

Question 8

How is an antibody library screened?

Answer 8

The library is exposed to an antigen of interest and antibodies that bind to the antigen are selected.

Question 9

What are scFvs?

Answer 9

Single-chain variable fragments, a type of antibody fragment consisting of the variable regions of the heavy and light chains connected by a flexible linker.

Question 10

Initials: scFvs

Answer 10

Single-chain Variable Fragments

Question 11

What is the benefit of using phage display for antibody discovery?

Answer 11

It allows for rapid and high-throughput screening of a large number of antibodies for specific antigen binding.

Question 12

What is affinity maturation in relation to phage display?

Answer 12

A process where antibodies with higher affinity for a specific antigen are evolved through repeated rounds of selection and mutation.

Question 13

What is a phage display library?

Answer 13

A collection of bacteriophages displaying a variety of peptides or proteins on their surface.

Question 14

What is a landscape phage?

Answer 14

A bacteriophage that displays a complex peptide library on its surface.

Question 15

What are Fab fragments in relation to antibody libraries?

Answer 15

Fragment antigen-binding, these are a type of antibody fragment that can be expressed in phage display libraries.

Question 16

Initials: Fab

Answer 16

Fragment Antigen-Binding

Question 17

How are specific antibodies identified in a phage display library?

Answer 17

Through biopanning, where phages displaying the desired antibody are enriched through repeated rounds of selection.

Question 18

What is an immunotube?

Answer 18

A tube coated with antigen used in the biopanning process to capture phages expressing the desired antibody.

Question 19

What is the process of elution in phage display?

Answer 19

The process of releasing captured phages from the antigen-coated surface during biopanning.

Question 20

What are the applications of phage display?

Answer 20

• Antibody discovery
• Vaccine development
• Drug discovery
• Protein engineering

Question 21

What are the limitations of phage display?

Answer 21

• Limited peptide size
• Biased library representation
• Constraints on protein folding