[26] High-throughput Screening Methods Flashcards
What is High-throughput Screening (HTS)?
A method for scientific experimentation especially used in drug discovery and relevant to the fields of biology and chemistry.
What is the main purpose of HTS?
To identify active compounds, antibodies, or genes which modulate a particular biomolecular pathway.
What does HTS involve?
The use of robotics, data processing and control software, liquid handling devices, and sensitive detectors.
What does HTS allow researchers to do?
It allows them to quickly conduct millions of biochemical, genetic, or pharmacological tests.
What is the output of HTS?
Through these tests, they can identify active compounds, antibodies, or genes that modulate a particular biomolecular pathway.
How is HTS used in drug discovery?
It’s used to quickly conduct millions of chemical, genetic or pharmacological tests to identify potential drug candidates.
What are the key components of HTS?
Robotics, liquid handling devices, microplate readers, and data processing and control software.
What is a microplate reader?
A device used to detect biological, chemical or physical events of samples in microtiter plates.
What is a potential drawback of HTS?
The generation of false-positive or false-negative results due to the complexity of the tests.
What is the initial step in HTS?
The creation of a robust and miniaturizable assay.
Why is miniaturization important in HTS?
It allows testing a larger number of samples with less reagent, saving cost and resources.
What is the role of robotics in HTS?
They automate sample handling, reducing time and eliminating human error.
