20. Microbio: Bacterial pathology Flashcards
Bacteria assoc pigment: red metachromic granules or babes ernst granules
Corynebacterium diptheriae
Bacteria assoc pigment: red
Serratia marcescens
Bacteria assoc pigment: bluish green; associated with NOSOCOMIAL (hospital acquired) infections
Pseudomonas aeruginosa
BQ: Bacteria assoc pigment: yellow sulfur granules
Actinomyces israeli
Metabolically active (maturing); not yet dividing. - nagaadapt palang yung microorganism sa environment
Lag phase
Cell division is rapid
- Bactericidal antibiotics act in this phase
Log phase/Exponential phase
Kill bacteria
- to take effect = cells must be in cell division stage
Bactericidal
Inhibition of growth and multiplication
Bacteriostatic
Rate of division = death rate
Stationary phase
Nutrients are greatly reduced, TOXINS increased
Death > multiplication
Death phase
BQ: What do you call the cell division of bacteria?
Binary fission (Asexual reproduction)
BQ: What are the small DNA molecules found in the microorganisms that are separated from their chromosomal DNA?
Plasmid
This is the process of transferring PLASMIDS from one microbe to another
Horizontal gene transfer or bacterial recombination
Direct contact and uses SEX PILI(used for attachment)
Conjugation
Type of horizontal gene transfer where DNA is left in envt then engulfed by recipient bacteria
Transformation
“F” for feces nlilalabas naiiwan sa envt
Uses bacteriophage or a bacterial virus to transfer plasmids
Transduction
“D” delivery boy: transfer
Bacteriophage = USER
- Phage genome combines with bacteria (Lysogenic cycle)
- When bacteria multiplies, viral DNA also multiplies
- Phage kills bacteria (LYTIC CYCLE)
Temperate phage
Bacteriophage = not useful to the bacteria
- phage kills bacteria (lytic cycle)
Virulent phage
BQ: What is the basic component of a CELL WALL? (bacterial cell wall)
Glycoproteins
Gram positive cell wall is _____ than gram negative
Gram positive thicker cell wall
Peptidoglycan of gram positive vs gram negative
Thick peptidoglycan positive
Thin peptidoglycan negative
Exotoxin of gram +
Attached to the peptidoglycan or murein LAYER
+eichoic acid = EXOtoxin (+)
ENDOtoxin of gram -
Activated immune response
Lipopolysaccharide (ENDOtoxin -)
What gram positive bacteria produces ENDOtoxin and EXOtoxin?
Lis+teria monocytogenes
With mycolic acid which is a waxy substance that is resistant to gram staining and decolorization
Acid fast
Is a waxy substance that is resistant to gram staining and decolorization
Mycolic acid
Gram staining procedure (VIAS)
Crystal violet (primary stain)
Iodine (violet)
Alcohol (decolorization)
Safranin/carbolfuchsin (red; counterstain)
Color of gram +
Violet
Color of gram -
Red
Streptococcus mutans: gram _
S. Mutans gram + violet
Neisseria gonorrhea: gram _
N. Gonorrhea gram - red
Acid fast staining procedure (CAM)
Carbolfuchsin (red primary stain)
Acid-alcohol
Methylene blue (blue counterstain)
Acid fast staining is aka
“Ziehl neelsen stain”
Color of acid fast bacteria
Red
Color of gram + and - in acid fast staining
Blue
Gram+ blue to violet
Normal bacteria of the SKIN
Staphylococcus
Grape like
Staphylococcus
Normal bacteria of the ORAL CAVITY
Streptococcus
Chain like
Streptococcus
Pus producing and resistant to drugs
Staphylococcus
Virulence factors of Staphylococcal infections
Protein A
Coagulase
Staphylokinase
Hyaluronidase
Virulence factors of Streptococcal infections
Streptokinase
Pneumolysin
M protein
Staph virulence factor: prevents complement action of complement proteins
Protein A
BQ: What staph bacteria that clots blood by activating Prothrombin to Thrombin??
(used to create CLOT)
Coagulase
BQ: What staph bacteria activates plasminogen producing plasmin??
(used to DISSOLVE CLOT)
Staphylokinase
What staph bacteria breaksdown hyaluronic acid; spreading factor
Hyaluronidase
Bone and bone marrow inflammation
Rx: moth eaten
Brodies abscess
Osteomyelitis
Causative agent of osteomyelitis
Staphylococcus aureus
Most common cause of acute endocarditis
Staphylococcus aureus
“A”cute - “a”ureus
BQ: What is the most common cause of ENDOCARDITIS?
Streptococcus viridans
(Su”B”acute - “V”iridans)
- KASI ITO YUNG MAS COMMON NA ENDOCARDITIS
Localized pus containing skin boil
Furuncle
Extensive form of furuncle
Carbuncule
Bacteria that causes food poisoning or gastroenteritis
Staphylococcus
BQ: What STREP bacteria activates plasminogen to plasmin?
Streptokinase
Cytotoxin mainly of Streptococcus pneumoniae
Pneumolysin
Antigen of group A Beta Hemolytic Streptococcus
M protein
2 classifications of streptococcal infections
Alpha hemolytic
Beta hemolytic
Produces “incomplete” hemolysis
Alpha hemolytic
“A”lpha - “A”incomplete
Produces “complete” hemolysis
Beta hemolytic
Alpha hemolytic uses _ to lyse blood cells INCOMPLETELY
Hydrogen peroxide
Beta hemolytic uses _ to lyse blood COMPLETELY
Streptolysin
Bacterial pneumoniae
Streptococcus pneumonia
Subacute endocarditis
Streptococcus viridans
1st colonizer of plaque
Strep. sanguis
1st colonizer of oral cavity (10-12hrs or *24hrs(sterile))
Strep. Salivarius
Most numerous bacteria in oral cavity
Strep salivarius
BQ: Caries
Strep mutans
What is the predominant bacteria in ENAMEL?
Strep mutans
What is the predominant bacteria in DENTIN?
Lactobacillus acidophilus*
-lactobacillus casei
What is the predominant bacteria in ROOT SURFACE
Actinomyces viscosus
Reaction between food and saliva
24-48 hrs after tooth brushing
Plaque
Colony of microorganisms
Biofilm
BQ: sugars in plaque
*Dextrans/glucans (sticky sugar:Adhesive)
Levans
BQ: Which of the ffg diseases will result to hospitalization?
Pansinusitis
Infection of lungs that may result to pulmonary edema
Pneumonia
2 types of pneumonia
Bacterial
Viral
Bacteria involved in bacterial pneumonia
Strep pneumoniae
Viral pneumonia: Adult
Influenza virus
Viral pneumonia: children
Respiratory syncytial virus
Example of influenza virus
Magaling mag mutate ung influenza virus: nagiiba yung shape palagi kaya nagkakaflu parin kahit ngpavaccine na
H1N1
Virulence factor in Group A Beta Hemolytic Strep
M protein (looks like myosin)
Most commonly involved GABS
Streptococcus pyogenes
Yellowish honey colored scabs in the skin
Impetigo
“Strawberry tongue”
Scarlet fever
Disease with strawberry tongue
Unknown cause
Ddx: with Scarlet fever
Kawasaki disease
3 diseases associated with Strep Pyogenes
Impetigo
Scarlet fever
Strep throat
Hypersensitivity to M protein debris, after the body has recovered from the actual infection
Post streptococcal infections
BQ: Generalized rheumatism
Characterized by polyarthritis, Pancarditis, Valvular damage - mitral valve > aortic valve, Chorea, Erythema marginatum
Rheumatic fever
5 charac features of Rheumatic fever
Polyarthritis Pancarditis Valvular damage - mitral valve > aortic valve CHOREA ERYTHEMA MARGINATUM
Mitral valve is aka
Bicuspid valve (LEFT)
Tricuspid valve - right side
Neurological manifestation of Rheumatic fever characterized by abnormal involuntary movements
Sydenham’s CHOREA
Pink torso rings
Erythema marginatum
BQ: Which of the ffg is post strep infection?
(post streptococcal) Glomerulonephritis
What are the 3 most common bacteria that uses COLLAGENASE and HYALURONIDASE?
SA - staph aureus
SP - Strep pyogenes
CP - clostridium perfringens
3 that has PSEUDOMEMBRANES
Corynebacterium diphtheria - (diphtheritic membrane)
Necrotizing ulcerative gingivitis
Pseudomembranous colitis
BQ: Punched out grayish pseudomembrane in interdental papilla
Necrotizing ulcerative gingivitis
Needs bacteriophage(bacterial virus) with diphtheria toxin to cause a disease
Corynebacterium
Necrosis pf pharyngeal and upper respiratory tract surface producing diphtheritic membrane (pseudomembranous membrane)
Corynebacterium diphtheria
Granules in Corynebacterium diphtheria
Red metachromic granules or “Babes Ernst granules”
Histologic app of Corynebacterium diphtheria
Chinese character
*(same with fibrous dysplasia)