M2 Flashcards
orderly INCREASE in the sum of all the compounds of an organism
GROWTH
what mode of reproduction is the BINARY FISSION
ASEXUAL REPRODUCTION
process of binary fission
- DNA replication
- cytoplasm elongate
- start of split
- split
- 2 identical daughter cells
how many is the product of binary fission
2 idential daughter cells
an increase in the number of SINGLE BACTERIA making up a population
CULTURE
is an increase in the size a growth among microorganisms
AN INCREASE IN THE SIZE IS NOT A TRUE GROWTH AMONG MICROORGANISMS
AVERAGE TIME required for the population, or biomass, to DOUBLE
GENERATION TIME / DOUBLING TIME
generation time / doubling time is _________ in every microorganism
NOT CONSTANT
BACTERIAS are said to undergo ________
EXPONENTIAL GROWTH / LOGARITHMIC GROWTH
PHASES of bacterial growth curve in order
LAG PHASE
LOG / EXPONENTIAL PHASE
STATIONARY PHASE
DEATH / DECLINE PHASE
a period where cells are DEPLETED of metabolites and enzymes as the result of the UNFAVORABLE CONDITIONS
LAG PHASE
- cells ADAPT to their new environment
- enzymes & intermediates are FORMED
- NO DIVISION happening but still active
- preparing themselves to divide
- bacteria undergo INTENSE METABOLIC ACTIVITY
LAG PHASE
during this phase, the cells are in a STEADY STATE. new cell is being synthesized at a CONSTANT RATE
LOG / EXPONENTIAL PHASE
the EXHAUSTION OF NUTRIENTS or the ACCUMULATION OF TOXIC PRODUCTS causes the growth to CEASE COMPLETELY
STATIONARY PHASE
- cells are now DIVIDING
- cells are in STEADY STATE & grow
- the stage where most bacterial cells are MOST METABOLICALLY ACTIVE
LOG / EXPONENTIAL PHASE
- period of EQUILBIRIUM
- # of deaths = # of new cells
- slow loss of cells through death and balanced by the formation of new cells
STATIONARY PHASE
cell viability begins to DECREASE at a DEFINED RATE
DEATH / DECLINE PHASE
GROWTH RATE:
lag phase
0
GROWTH RATE:
log / exponential phase
constant
GROWTH RATE:
stationary phase
0
GROWTH RATE:
death / decline phase
negative
stationary phase is also called
PLATEU
3 major mechanisms for generating metabolic energy
FERMENTATION
RESPIRATION
PHOTOSYNTHESIS
characterized by substrate phosphorylation
FERMENTATION
analogous to the COUPLING of an ENERGY-DEPENDENT process to the discharge of a battery
RESPIRATION
like respiration but the REDUCTION OF NA OXIDANT via a specific series of ELECTRON CARRIERS establishes the PROTON MOTIVE FORCE
PHOTOSYNTHESIS
- process of PROPAGATING microorganisms by providing the PROPER environmental conditions
CULTIVATION
NUTRITIONAL BACKBONE for every microorganism
CARBON
organisms that DO NOT REQUIRE organic nutrients for growth. these are CAPABLE of MAKING THEIR OWN FOOD
AUTROTROPHS
organisms that use an INORGANIC SUSBTRATE
CHEMOLITHOTROPHS
REQUIRE organic carbon for growth, and it must be in the form that can be assimilated
HETEROTROPHS
major component of PROTEINS, NUCLEIC ACID, and other compounds
NITROGEN
- key component in plants for PHOTOSYNTHESIS
- a property UNIQUE to PROKARYOTES
NITROGEN FIXATION
increase in the population/number of cells
TRUE GROWTH
refers to the INCREASE in the NUMBER OF CELLS, not the size of the cell
MICROBIAL GROWTH
division time of E. coli
15 mins
the time when microorganism is ACQUIRED until the appearance of FIRST SYMPTOMS
INCUBATION PERIOD
proper environmental condition for microorganism to grow, optimal for growth
INCUBATOR
Most microorganisms assimilate nitrogen in the form of ______ (NH3) through membrane diffusion.
AMMONIA
a component of many organic cell substance forming part of several coenzymes and found in the CYSTEINYL and METHIONYL side chains of proteins.
SULFUR
required as a component of ATP, nucleic acids and coenzymes like NAD, NADP and flavins.
PHOSPHORUS
found in chlorophyll molecules
MAGNESIUM
Mg2+
part of the coenzyme of the cytochromes and peroxidases
IRON
Fe2+
found in POROPHYRIN DERIVATIVES
MAGNESIUM (Mg2+)
FERROUS (Fe2+)
essential for the function and integrity of RIBOSOMES
Mg2+ & K+
required constituent of GRAM-POSITIVE CELL WALLS
Ca2+
required for the growth of MARINE ORGANISMS
Na+
Other minerals include Mn+2, Mo+2, Co+2, Cu+2 and Zn+2 are required in ________
TRACE QUANTITIES (small amounts)
compounds that CHELATE IRON and promote its transport as a soluble complex
SIDEROPHORES
an organic compound which a CELL MUST HAVE TO GROWN but which it is UNABLE TO SYNTHESIZE.
GROWTH FACTOR
ENERGY SOURCE, CARBON SOURCE:
Photoautotrophs
Light, Carbon dioxide
ENERGY SOURCE, CARBON SOURCE:
Photoheterotrophs
LIGHT, ORGANIC COMPOUNDS
ENERGY SOURCE, CARBON SOURCE:
Chemoautotrophs or Lithotrophs
INORGANIC COMPOUNDS, CARBON DIOXIDE
ENERGY SOURCE, CARBON SOURCE:
Chemoheterotrophs or Heterotrophs
ORGANIC COMPOUNDS, ORGANIC COMPOUNDS
most organisms have a _______ optimal pH range
FAIRLY NARROW
microorganisms that live in a pH range of 6.0 to 8.0
NEUTRALOPHILES
microorganisms that live in a pH of 3.0
ACIDOPHILES
microorganisms that live in a pH of 10.5
BASOPHILES / ALKALIPHILES
OPTIMAL GROWTH TEMPERATURE:
Psychrophiles / Psychrophilic
-5 to 15C
OPTIMAL GROWTH TEMPERATURE:
Psychrotrophs
20 - 30C
OPTIMAL GROWTH TEMPERATURE:
Mesophilic / Mesophiles
30 - 37C
OPTIMAL GROWTH TEMPERATURE:
Thermophilic / Thermophiles
50 - 60C
OPTIMAL GROWTH TEMPERATURE:
Hyperthermophilic / Hyperthermophiles
above the temp of boiling water
grows best at LOW temperatures (COLD environment)
PSYCHROPHILES, PSYCHOTROPHS
can survive human body temp
MESOPHILES
MICROBIAL SPECIES that grows from low temp to high temp (in order)
Psychrophile
Psychrotrophs
Mesophile
Thermophile
Hyperthermophile
microorganisms that can cause DISEASES in HUMANS
NEUTRALOPHILE
a NATURAL BARRIER to infection because it is ACIDIC
STOMACH
needs oxygen
AEROBIC
does not need oxygen
ANAERBOIC
requires OXYGEN as HYDROGEN ACCEPTOR
OBLIGATE AEROBES
needs oxygen
OBLIGATE
able to live aerobically and anaerobically
FACULTATIVE ANAEROBES
prefer oxygen but can also live without it
FACULTATIVE ANAEROBES
requires SMALL AMOUNTS OF OXYGEN (2-10%) & enzymes
MICROAEROPHILIES
SENSITIVE to OXYGEN inhibition
OBLIGATE ANAEROBE
HATES oxygen, does not have enzymes
OBLIGATE ANAEROBE
HATES OXYGEN but can TOLERATE it (bcoz walang use and oxygen sa kaniya)
AEROTOLERANT
indifferent to oxygen, can grow in its presence but do not use it as hydrogen acceptor
AEROTOLERANT
ENZYMES FOR O2 DETOXIFICATION:
Obligate aerobe
Catalase: hydrogen peroxide 2H2O2 –> hydrogen peroxide 2H2O + superoxide O2
ENZYMES FOR O2 DETOXIFICATION:
Facultative anaerobe
Catalase, superoxide dismutase
ENZYMES FOR O2 DETOXIFICATION:
Obligate anaerobe
Neither catalase nor superoxide dismutase in most
ENZYMES FOR O2 DETOXIFICATION:
Microaerophile
small amounts of catalase & superoxide dismutase
ENZYMES FOR O2 DETOXIFICATION:
Aerotolerant
superoxide dismutase
Catalase: 2H2O2 –> 2H2O + O2
Superoxide dismutase: 2O2- + 2H2+ —> O2 + H2O2
OBLIGATE AEROBE
Catalase, superoxide dismutase
FACULTATIVE ANAEROBE
Neither catalase or superoxide dismutase in most
OBLIGATE ANAEROBE
small amounts of catalase & superoxide dismutase
MICROAEROPHILE
superoxide dismutase
AEROTOLERANT
water —> high concentration
PLASMOLYSIS
natural by-products of aerobic metabolism
HYDROGEN PEROXIDE H2O2
SUPEROXIDE O2-
MARINE BACTERIA that requires growth in the presence of HIGH SALT concentrations are called
HALOPHILES
organisms capable of growing in high concentrations of SUGARS are called
OSMOPHILES