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Pathogenic Microbiology > Lab 3 > Flashcards

Lab 3 Flashcards

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1
Q

Streptococcus ID and Isolation

Blood agar was used

What kind of medium is it?

What technique did we use with the plate?

A

Enrichment and differential

Quadrant Streak Isolation

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2
Q

We swabbed our throats looking for Streptococci

What are the possible results

A

Alpha: Green area. Reduced hemolysis around colonies Pin head sized.
Possibly Streptococcus mitis / Streptococcus pneumonia

Beta: Clear Zones under and around colony RBC Destroyed Pin head.
Possibly: Staph aureus / Streptococcus pyogenes

Gamma: No hemolysis Larger colonies
Possibly: Staphylococcus epidermidis

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3
Q

Alpha hemolysis: Describe
Possible species

A

Green area. Reduced hemolysis around colonies Pin head sized.
Possibly Streptococcus mitis / Streptococcus pneumonia

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4
Q

Beta hemolysis: Describe
Possible species

A

Clear Zones under and around colony RBC Destroyed Pin head.
Possibly: Staph aureus / Streptococcus pyogenes

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5
Q

Gamma hemolysis

A

No hemolysis Larger colonies
Possibly: Staphylococcus epidermidis

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6
Q

In blood agar why do we stab the agar?

A

To allow less oxygen to encourage growth of bacteria

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7
Q

Mannitol contains how much sodium chloride

A

7.5%

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8
Q

What are fomites

A

Inanimate objects that may contain bacteria

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9
Q

Which species cause Beta lysis ( Clear Zone)
Does this destroy the blood cell

A

Staphylococcus aurus
Streptococcus pyogenes

Yes

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10
Q

Which species causes Alpha lysis ( Green or brown)
Is this destruction of the RBC

A

Streptococcus mitis
Streptococcus pneumonia

Partial

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11
Q

Which species causes Gamma lysis(No color change)
Does this destroy it?

A

Staphylococcus epidermidis
No

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12
Q

What kind of media is Mannitol Salt plate?
How so?
Which type of bacteria were we looking for?

A

Selective 7.5% NaCl selects for Staphylococcus

Differential: Mannitol when fermented bacteria produce acid which turn pH indicator Phenol red to a Yellow color

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13
Q

What does a Pink / Yellow color on Mannitol Salt Plate indicate?

A

Pink no break down of sugar
Yellow break down of sugar

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14
Q

Type of bacteria we were looking for in the blood agar experiment with the quadrant Streak taken from throat

A

Streptococci

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15
Q

Type of bacteria and medium used in the Nose, Formite, and Unknown.

What was the differential process

What was the selective process

Type of medium used

A

Staphylococci

Breaking down of the sugars (mannitol)

7.5% salt

Mannitol Salt plate

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16
Q

The Mannitol Salt plate was used for this bacteria

The blood agar was used for this bacteria

In the earlier experiments NOT the unknown

A

Staphylococci

Streptococci

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17
Q

Mannitol Salt Plate

Used to ID this type of Bacteria

Yellow = this bacteria
Pink = this bacteria

A

Staphylococcus

Staphylococcus aureus (Breakdown)

Staphylococcus epidermidis (No Breakdown)

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18
Q

What does DSLB and SSLB mean and which 2 species, know as what, was it looking for?

A

DSLB is 10ml “test water sample”
SSLB is 1 & .1 ml “test water sample

Intestinal bacteria “ E. coli & Enterobacter aerogenes” Coliforms

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19
Q

What is the name of the mechanism in which the CO² was collected in while looking for Coliforms

A

Durham Tube

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20
Q

The Presumptive test for fecal contamination has 3 indicators for a positive test, name them

A

Color change from Pink to Yellow
Cloudy growth
Gas bubble in Durham tube (due to Break down of Lactose)

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21
Q

10ml pipette is this color
1ml pipette is this color

A

Green
Blue

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22
Q

What were the two types of agars we inoculated in the Bacterial Water Exam

What are the positive results for coliforms?

A

Levine EMB agar: Coliforms produce Large Blue-Black colonies with a green metalic sheen for E coli

Endo agar: Coliform colonies produce Pink to Rose-Red colonies.

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23
Q

What is the gram status and differential mediums used for coliforms?

A

Gram-negative
Lactose fermentation Presumptive
Levine EMB & Endo agar Confirmed

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24
Q

The Presumptive test for Coliforms: is also used to determine this ______ which is the amount of coliforms present per 100ml water

A

MPN

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25
Q

Initial dilution of lettuce given to class

A

1:10

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26
Q

How many mls were transferred from Inital sample to A in lettuce dilution

What was As dilution

How many mls were transferred from A to B
What was its total dilution

A

. 1ml
1:1000

.1ml
100,000

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27
Q

4 plates were used in the lettuce sample to grow bacteria

What is the dilution if .1 ml is platted from 1:1,000 dilution

A

10,000

28
Q

How much does 1ml of water weigh

A

1 gram

29
Q

Why are EMB and endo agar used as a confirmation test

A

They select for Gram-negative and don’t allow Gram-positive to grow

30
Q

Citrobacter, Enterobacter, Klebsiella, Escherichia are all this type of microbe

A

Coliforms

31
Q

Type of plate / plate count used in bacterial count of a food product

A

Standard methods agar
Heterotrophic plate count

32
Q

The medium hetrotrophic gives what nutrient to support the growth of bacteria

A

Organic carbon

33
Q

EMB agar is selective for gram-negative bacteria.

How does it do this?

How does it differentiate?

A

The dye methylene blue inhibits the growth of gram positive Selective

Eosin and methylene blue are pH indicator dyes when lactose gets broken down it produces acid Differential

34
Q

What does EMB stand for in the agar

A

Eosin methylene blue agar

35
Q

The selectivity of _____ is due to the sodium sulfite/basic fuchsin combination which results in the suppression of gram-positive microorganisms.

A

Endo agar

36
Q

Which metabolic process makes EMB and endo agar differential

A

Lactose fermentation

37
Q

The differential ingredient in MSA is…

The selective ingredient is…

The acidity of the media will cause the pH indicator, ______, to turn yellow.

A

The sugar Mannitol

7.5 % NaCl

Phenol Red

38
Q

In the lettuce experiment with 4 plates

If .1 ml from B (1: 100,000) is plated what will the concentration be

A

1,000,000

39
Q

In the unknown Gram Positive

Cocci get put on this type of plate
Possible outcomes

Rods on this type of plate

A

MSA: Cocci
Pink Staphylococcus epidermidis(pink)
Yellow Staphylococcus aureus (yellow)

Blood agar: Rods
Beta hemolysis: Bacillus cereus
Gama hemolysis: Bacillus megaterium

40
Q

Unknown Gram positive Rods got plated on what?

What were the 2 possible outcomes and species.

A

Blood agar

Beta hemolysis: Bacillus cereus
Gama hemolysis: Bacillus megaterium

41
Q

Unknown experiment

Cocci Gram-positive got this type of plate
2 possible outcomes

A

MSA plate Mannitol Salt plate

Pink: Staphylococcus epidermidis
Yellow: Staphylococcus aureus

42
Q

If 2 plates were done in the Unknown

Which type of staining process was done.

Which stain was used

A

Simple Stain

Air dry
Heat fix

Methylene blue 1 minuto

43
Q

S aureus, S epidermidis, B megaterium, B cereus

All have this gram staining property

A

Gram positive

44
Q

Alcaligenes faecalis, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris

All have this gram staining property

A

Gram negative

45
Q

Thioglycolate is used to determine this in the Unknown Gram negative

A

Oxygen requirements

46
Q

In the gram negative Thycogyclate which was the:

Obligate aerobe

Facultative anaerobe

A

Alcaligenes faecalis ( Obligate Aerobic)

Escherichia coli, Enterobacter aerogenes, Proteus vulgaris
(Facultative anaerobe)

47
Q

Gram negative Unknown

Citrate agar

Which color indicates no citrate utilization

Which indicates use of citrate

Inoculation instrument

A

Green = (-) or no use of citrate

Blue/Black = (+) break down of citrate

Loop

48
Q

Which gram negative species use citrate turning the medium Blue/Black

Which don’t use citrate leaving the agar a Green

A

Enterobacter aerogenes (Blue/ Black) Use citrate

E. coli, Proteus vulgaris, Alcaligenes faecalis (Green) Don’t use citrate

49
Q

In the gram negative Unknown

Urease Production is measured in the Urea Slant Agar

Which type of inoculation instrument was used.

What is a Negative/ Positive indicator of Urease Production

A

Loop

Orange / Pink: Negative

Flaming Pink: Positive

50
Q

On the Gram negative Unknown

Thioglycolate & Motility Media

Both used this instrument to inoculation

A

Straight needle

51
Q

Gram negative Unknown

Urease Production

These bacteria produced Urease with a Flamming Pink Result

These bacteria did not produce Urease with a light pink / orange Result

A

Proteus Vulgaris Produced Urease Flamming Pink

Alcaligenes faecalis, E coli, Enterobacter aerogenes. No Urease Production light orange

52
Q

How were the caps adjusted in the Gram-negative unknown

A

Loose cap

53
Q

Motile organisms are able to move about the semi-solid medium resulting in a ______ growth throughout the medium.

Non-motile organisms will remain isolated at the inoculation point and will appear as pink or red growth along the stab line.

Which bacteria were motile

A

pink or red diffuse

All

54
Q

Thioglycolate is an enriched non-selective broth

T or F

A

T

55
Q

Motility test media is which type of media

A

Differential

56
Q

This type of (Streaking) is done on the MSA plate:

Blood agar:

A

MSA: Zig zag

Blood agar: Quadrant Isolation Streak

57
Q

This is the type of plate used in the Handwashing lab

is a general purpose plating medium used for the isolation, cultivation, and maintenance of a variety of fastidious and non- fastidious microorganisms.

A

Tryptic Soy Agar (TSA)

58
Q

Normal flora from handwashing on Tryptic Soy Agar (TSA) Plate had this appearance

Possible pathogens this appearance

A

Small white dots (Normal flora)

Larger Red (Possible Pathogen)

59
Q

Type of (Streaking) used on Levine EMB & endo agar

A

Quadrant streak

60
Q

How were the counts in the Bacterial food count done

A

CFU

61
Q

What is the definitive test for S. aureus

A

Colagulase

62
Q

Which gram positive bacteria are positive on a TSA (blood agar plate)

A

Both

Staphylococcus Aureus ( Beta hemolysis) (Giant colonies)& Staphylococcus epidermidis (Gamma hemolysis)(small white colonies)

Bacillus megaterium ( Gamma hemolysis) & Bacillus cereus (Beta hemolysis)

63
Q

Which gram positive bacteria are positive on MSA plate.

Which are negative

A

Positive: Staphylococcus aureus (Yellow-breakdown)

Staphylococcus epidermidis (Pink-no breakdown but growth)

Negative
Bacillus megaterium & Bacillus cereus

The 7.5 salt kills them

64
Q

_____ broth is the medium we used as a presumptive test for coliform bacteria / water test

A

DSLB / SSLB

Double/ Single Strength Lactose Broth

65
Q

Which unknown organisms will grow on PEA (phenyethyl alcohol agar).

A

Will Grow: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus megaterium, Bacillus cereus

Will not grow: Alcaligenes faecalis, Enterobacter aerogenes, Escherichia coli, Proteus vulgaris

66
Q

Which unknown will grow in EMB agar (Eosin Methylene Blue)

A

Gram-negative will Grow: Alcaligenes faecalis, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris

Gram-positive will not grow: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus megaterium, Bacillus cereus

67
Q

Which medium grew gram-positive

Cocci

Bacillus

A

Cocci: MSA (Mannitol Salt plate)

Bacillus: Blood Agar

Pathogenic Microbiology (31 decks)

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