Haemostasis Flashcards
Define haemostasis
The cellular and biochemical process that enables cessation of bleeding in response to injury
What are the 3 roles of haemostasis?
To prevent loss of blood from intact vessels
To arrest bleeding from injured vessels
To enable tissue repair
What are the 4 mechanisms of haemostasis?
Vessel constriction
Primary haemostasis
Secondary haemostasis
Fibrinolysis
Describe the first mechanism of haemostasis
Vessel constriction occurs at the site of injury to limit blood flow, usually in vascular smooth muscle
Describe the second mechanism of haemostasis
Primary haemostasis is the formation of a temporary platelet plug, this occurs via:
Exposure of collagen on the endothelium wall at the site of injury
Platelets attach either directly via g1pIa receptor or indirectly via VWF and gp1b receptor
Platelets aggregate after release of contents, thromboxane is synthesised from arachidonic acid
This activates platelets by activating g1pIIb or IIa receptors
What are the 2 ways platelets attach at the endothelium wall? What receptors are involved?
Directly via g1pIa
Indirectly via VWF and gp1b receptor
What are the 3 components of primary haemostasis?
VWF
Platelets
Vessel wall
What content do platelets release?
ADP and thromboxane
What is thromboxane synthesised from?
Arachidonic acid
What is the 3rd mechanism of haemostasis?
Secondary haemostasis, this is the formation of a permanent platelet plug
What is the scientific term for secondary haemostasis?
Coagulation
What is the 4th mechanism of haemostasis?
Fibrinolysis, it involves breakdown of the fibrin clot and vessel repair so vessel integrity is restored
What is normal haemostasis the balance between?
Fibrinolytic factors and anticoagulant proteins vs coagulant proteins and platelets
What will a tip in the haemostatic balance cause either way?
Excess coagulant proteins/platelets= thrombosis
Excess fibrinolytic factors/anticoagulant proteins= bleeding
What are ways bleeding will occur?
Excess fibrinolytic factors/anticoagulant
Reduced nos of platelets and coagulant proteins, this could occur via
1) Reduced production (congenital or acquired)
2) Increased clearance or consumption (genetic or acquired)
What is the name of the condition where someone has low numbers of platelets?
Thrombocytopenia
What are the 3 main ways thrombocytopenia can arise?
Bone marrow failure causing reduced production
Increased clearance
Pooling and destruction in the spleen
How can bone marrow failure cause thrombocytopenia?
Leukaemia- white cells infiltrate the bone marrow and so platelets cannot be produced
B12 deficiency- causes megaloblastic anaemia so normal haemopoeisis ceases
How can accelerated platelets clearance manifest? Describe the pathophysiology of the conditions
ITP- platelets are destroyed in circulation by auto antibodies
DIC- small clots form all over the body in small vessels using up a lot of platelets
How does aspirin cause impaired platelet function?
It reversibly blocks the action if cyclo-oxygenase which reduces platelet aggregation
What drugs impair the function of platelets?
NSAIDs, clopidrogel, aspirin
How long does a dose of aspirin last?
7 days
How does clopidrogel impair platelet function?
Irreversibly blocks ADP receptor P2Y12 on platelet cell membranes
What is Glanzmann thrombothaenia?
Absence of GPIIb/IIIa receptors on platelets
What is Bernard Soullier syndrome?
Absence of GPIb receptors
What is storage pool disease?
A group of disorders where there is reduced granular contents in platelets
What are the 3 subcatagories of disorders of primary heamostasis?
Ones that affect
platelets (reduced numbers of impaired function)
or VWF
or the vessel wall
What is von willebrand disease?
When there is reduced quantity or function of VWF
How is von willebrand disease inheroted?
Autosomal
What are the different types of von willebrand disease?
Type 1 an type 3 are deficiency
Type 2 is abnormal function
What are the 2 functions of VWF in haemostasis?
Bind to collagen at injury site and capture platelets
Stabilise factor VIII
What may be low if VWF is low?
Factor VIII
What are inherited conditions that affect the vessel wall and cause disorders of primary heamostasis?
Hereditary haemorrhagic telangiectasia, Ehlers Danlos Syndrome and other connective tissue disorders
What are acquired conditions that affect the vessel wall and cause disorders of primary heamostasis?
Steroid therapy
Vasculitis
Scurvy
How does bleeding occur with disorders of primary haemostasis?
Immediate bleeding that is prolonged with cuts
Nose bleeds
Gum bleeds
Heavy menstrual bleeding
When do purpura appear?
If there are platelet or vascular problems
How does bruising change if there are disorders of primary haemostasis?
Bruising is spontaneous and may be easy
What is the difference between petechiae, purpura and ecchymosis?
Petechiae are < 3mm
Purpura are 3-10mm
Ecchymosis (bruises) are >10mm
What might severe VWD cause? Why?
Haemophilia like bleeding due to low factor VIII
What will APTT and PT (coagulation screens) in those with disorders of primary haemostasis be?
Normal
How are disorders of primary haemostasis treated if theres failure of production or function
Replace whats missing
Stop drugs eg aspirin and NSAIDs
How are disorders of primary haemostasis treated if theres immune destruction?
Immunosupression eg with prednisolone
Splenectomy in ITP
How are disorders of primary haemostasis treated if theres increased consumption?
Treat the cause
Replace whats necessary
How does desmopressin help as a haemostatic treatment?
It is a vasopressin analogue and increases stores of VWF
What is the normal range of platelets?
100-400 (x 10^9/L)
What is the other name for secondary haemostasis?
Coagulation
What is the role of coagulation
To generate thrombin (IIa) which converts fibrinogen to fibrin
What are the 3 tyoes of coagulation disorder?
Deficiency of coagulation factor production
Dilution
Increased consumption