biological molecules tests Flashcards
test for glucose?
1) add benedicts solution to a sample of food
2) place in a water bath at 80 degrees celsius for 5 minutes
3) colour change: blue-> brick red
test for glucose needs to be in a water bath at what temperature and for how long?
80 degrees celsius and 5 minutes
if glucose is present but in a lower concentration, what colour will it be?
blue to green, yellow or orange
test for starch?
1) add a few drops of orange iodine solution to the sample on a spotting tile
2) a blue- black colour indicates the presence of starch
what is the test for proteins?
1) add 2cm3 of water to a food sample and shake
2) add an equal volume of dilute potassium hydroxide and shake
3) add 2 drops of 1% copper sulfate solution
4) if protein is present then a pale purple colour will form
what is the test for lipids?
1) place the food sample in a test tube
2) add a small volume of ethanol and shake to dissolve any lipid in the alcohol
3) add an equal volume of water
4) a cloudy white colour - formed by the emulsion- will indicate the presence of lipids