biological molecules tests Flashcards

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1
Q

test for glucose?

A

1) add benedicts solution to a sample of food
2) place in a water bath at 80 degrees celsius for 5 minutes
3) colour change: blue-> brick red

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2
Q

test for glucose needs to be in a water bath at what temperature and for how long?

A

80 degrees celsius and 5 minutes

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3
Q

if glucose is present but in a lower concentration, what colour will it be?

A

blue to green, yellow or orange

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4
Q

test for starch?

A

1) add a few drops of orange iodine solution to the sample on a spotting tile
2) a blue- black colour indicates the presence of starch

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5
Q

what is the test for proteins?

A

1) add 2cm3 of water to a food sample and shake
2) add an equal volume of dilute potassium hydroxide and shake
3) add 2 drops of 1% copper sulfate solution
4) if protein is present then a pale purple colour will form

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6
Q

what is the test for lipids?

A

1) place the food sample in a test tube
2) add a small volume of ethanol and shake to dissolve any lipid in the alcohol
3) add an equal volume of water
4) a cloudy white colour - formed by the emulsion- will indicate the presence of lipids

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