Trp Operon - Part I Flashcards
Prokaryotic cells cannot carry out what protein synthesis process?
RNA processing
What is an operon?
A group of linked structural genes with common promoter and operator.
Transcribes as a single unit.
In what organisms are operons found?
Prokaryotic organisms (Bacteria)
What bacteria are we focusing on?
E. coli (Escherichia coli)
What is tryptophan?
An amino acid
How can humans get tryptophan?
Through environment (eating)
How can E.coli get tryptophan
- Ingest through environment
- Synthesise it
How many structural genes does the trp operon have?
5 sturctural genes
What section of gene does the trp operon possess o regulate gene expression?
Operator
Why does trp operon need to be regulated?
To not produce excess tryptophan and thus not waste energy.
Describe structure of trp operon chronologically
Ptrp (Promoter region) - Otrp (Operator sequence) - Leader sequence - Attenuator region - start triplet - trpE gene - trpD gene - trpC gene - trpB gene - trpA gene - stop triplet
Describe where Transcription and Translation occur in prokaryotes
- No nucleus present so in cytoplasm
- Both processes happen in same location at around same time
