Synthesising DNA Flashcards
1
Q
What are the 3 ways to synthesise DNA?
A
- DNA Replication
- Reverse transcription
- DNA Synthesisers
2
Q
Describe the 1st step in Reverse Transcription
mRNA
A
- Enzyme Reverse Transcriptase uses mRNA as template to build single stranded DNA that has complementary base sequence.
3
Q
Describe 2nd step in Reverse Transcription
Making second strand
A
DNA Polymerase builds the second strand using single-stranded DNA already created.
4
Q
Describe 3rd step in Reverse Transcription
Product
A
- DNA produced is known as complementary DNA (cDNA)
5
Q
How does complementary DNA of differ to normal DNA?
A
It does not have introns
6
Q
Describe 1st step in DNA Synthesis
What is needed?
A
- Free nucleotides are needed by machine
- Desired base sequence needs to be known
7
Q
Describe 2nd step in DNA Synthesis
A
- Instrument/machine called DNA synthesisers can join nucleotide bases in pre-defined order.
- Length of DNA created can be greater than 100 bases.
8
Q
What are the benefits of using DNA synthesisers to make DNA?
A
- No template strand required.
- No DNA Polymerase enzyme required.
