Terminal Respiration Flashcards
What is the only site of oxidative phosphorylation
In the mitochondria
What is the glycerol phosphate shuttle
NADH unable to cross mitochondrial membrane
Passes e- to FADH2 via G3P
Generates less ATP per mol than oxidation of NADH
What is involved in the electron transport chain
Complexes I, II,III and IV
What complexes directly pump protons into intramembrane space
I,III and IV
What is the function of Complex I
Oxidises NADH
Passes e- to Coenzyme Q(ubiquinone)
Co-emzyme Q Passes e- to ubiquinol (Complex III)
Uses redox centres
e- release energy which is used to pump H+ ions across membrane
What is the function of Complex II
Oxidises FADH2
Passes e- to coenzyme Q -> ubiquinol
What is the function of Complex III
Takes e- from ubiquinol->cytochrome C
1 Ubiquinol->2 cytochrome c molecules
Pumps protons
What is the function of Complex IV
Takes e- from cytochrome c-> molecular O2
Pumps protons
How is the energy stored in H+ gradient used
Electron motive force
ATP synthase
What is the function of ATP synthase (ATPase)
Protons move from intermembrane space down cg to matrix of mitochondria at certain sites
ATPase found at these sites
Converts ADP + Pi to ATP
Final stage in food metabolism into energy
What is the structure of ATP synthase
2 parts:
F0 - membrane bound proton conducting unit
F1 - protrudes in matrix
Catalyst for ATP synthesis
How many H+ do complexes I,III,IV move from matrix->intermembrane space
10H+
How many ATP are produced for every 3H+ atp synthase moves back into matrix
1 ATP
What is the difference between coupling and uncoupling
Coupled - E.t.c coupled to ATP synthesis
Uncouples - No ATP generated from E.t.c
