Introduction to Medical Microbiology Flashcards

1
Q

What are the 5 possible infecting agents?

A

Bacteria
Viruses
Fungi
Parasites
Prions

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2
Q

What is the specimen collection for a urinary tract infection?

A

Mid stream urine

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3
Q

What is the specimen collection for a chest infection?

A

sputum

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4
Q

What is the specimen collection for tonsillitis/pharyngitis

A

Throat swab

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5
Q

What is the specimen collection for the site of infection or a wound?

A

Swab or pus

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6
Q

What is the specimen collection for diarrhoea?

A

Faeces

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7
Q

What is the specimen collection for bacteraemia?

A

Blood culture

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8
Q

What is the specimen collection for meningitis?

A

CSF

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9
Q

What does unstained microscopy allow you to see?

A

Pus cells (urine, CSF)
Parasites (faeces)

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10
Q

What does a gram stain allow you to see in microscopy?

A

Bacteria yeast and fungi

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11
Q

What does a ZN stain or an auramine stain allow you to see?

A

Mycobacteria

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12
Q

What infecting agent is not visible in light microscope?

A

Viruses

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13
Q

What is the difference in the function between gram stain microscopy and culture?

A

Microscopy is rapid, insensitive, can’t identify a particular species

Culture is slower, more sensitive, conditions suitable for expected species

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14
Q

How is the species identified?

A

Observable characters - Morphological, physiological, biochemical

DNA tests

Typing - determines the strain within the species

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15
Q

What are the sterile sites within the body?

A

Brain Heart Liver Kidney

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16
Q

What are the non-sterile sites in the body?

A

Mouth Oesophagus Lungs Stomach
Mols are dirty:)

17
Q

How are viral infections diagnosed?

A

Antigen detection
Molecular methods including real time PCR
Serology to determine immunity

18
Q

§- What are the three different types of parasites?

A

Protozoa - malaria
Helminths (worms)
Arthropods - lice

19
Q

§ - How can parasites be diagnosed?

A

Microscopy of different stages - parasites, cysts and ova
- blood films for malaria
Culture rarely possible
Sometimes serology is useful

20
Q

Give some examples of healthcare acquired infection

A

Meticillin resistant Staph Aureus - MRSA
Clostridium difficile
Noroviruses
ESBL’s - Organisms with extended spectrum beta lactamases