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Bacte LEC > Serratia > Flashcards

Serratia Flashcards

1
Q

Pigment production at room temperature. “Prodigiosin” – pink-to-red in color

A

§ Serratia marcescens
§ Serratia rubidea
§ Serratia plymuthica

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2
Q

– emits a dirty, musty odor resembling “rotten potatoes”

A

Serratia odorifera

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3
Q

Serratia are all

A

Sucrose fermenters

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4
Q

Note: All Serratia species except for______ are late lactose fermenters, positive for
ONPG.

All are DNAse, Lipase, and Gelatinase positive (differential for other members of the family Enterobacteriaceae.

Serratia species are known for their resistance to a wide range of antimicrobials.

A

Serratia fonticola

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5
Q

– the most clinically significant species
§ Frequently found in hospital-acquired infections of the urinary tract

§ Bacteremic outbreaks in nurseries and cardiac surgery and burn units.

§ Contamination of antiseptic solution used for joint injections has result in an epidemic of septic arthritis.

A

Serratia marcescens

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6
Q

Serratia odorifera biogroups

– isolated predominantly from the respiratory tract and is positive for the fermentation of sucrose and raffinose, and is positive for ornithine decarboxylation

A

§ S.odorifera biogroup 1

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7
Q

Serratia odorifera biogroups

– isolated from blood and CSF and is negative for the fermentation of sucrose and raffinose, and negative for ornithine decarboxylation.

o 50% of isolates are indole positive

A

§ S.odorifera biogroup 2

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8
Q

Serratia marcescens
Laboratory Diagnosis
Biochemical reactions
TSI:
IMVIC:
Urease:
Lipase:
DNAse:
Gelatinase:
MaConKey: Clear colonies (Lactose negative

A

A/A or K/A, H2S negative

      • +

Negative

Positive

Positive

Positive

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9
Q

Resembles Enterobacter but differentiated by its inability to ferment lactose, sucrose, sorbitol, and raffinose

Differentiated from Serratia because it is
DNAse and Lipase (-)

A

Hafnia alvei (Enterobacter alvei)

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10
Q

– MAJOR CHARACTERISTICS OF Hafnia

A

Delayed positive citrate reaction

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11
Q

Tribe Proteeae
General Characteristics
Comprised of the genera:

A

Proteus, Providencia, and Morganella (PPM)

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12
Q

Widely distributed in the environment, are normal intestinal flora, and are recognized as opportunistic pathogens.

A

Tribe Proteeae

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13
Q

Distinguished from other members of the Enterobacteriaceae by their ability to
deaminate phenylalanine deaminase (PAD +)

None of the members of this tribe ferment lactose

A

Proteus, Providencia, and Morganella (PPM)

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14
Q

(2) are widelyrecognized human pathogens

Isolated from urine, wounds, ear, and blood

Responsible for 3% of UTI in the U.S
§ Ascend the urinary tract causing infections both in the lower and upper tracts

§ Can infect proximal kidney tubules and can cause acute glomerulonephritis particularly in patients with urinary tract defects or catheterization

A

Proteus mirabilis and Proteus vulgaris

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15
Q

– lead to struvite kidney stones

A

Urease activity of Proteus

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16
Q

easily identified by their
swarming colonies on culture media

A

Proteus mirabilis and Proteus vulgaris

17
Q

– thought to play a role in the ascending nature of Proteus-associated UTI

A

Swarming ability

18
Q

– produce a distinct “burnt chocolate” odor

A

Swarmers

19
Q

When different Proteus species swarm towards each other,
a line of inhibited growth results where strains meet.

A

Diene’s Phenomenon

20
Q

This line of inhibited growth results from the production of
and sensitivity to different types of bacteriocins, namely,
proticines, produced by different strains of Proteus species

A

Diene’s Phenomenon

21
Q

antigens are used in the diagnosis of rickettsial disease

are cross-reactive with Rickettsia antigens

A

Proteus

22
Q

Proteus antigens are used in the____– an agglutination test to differentiate rickettsial
disease.

A

Weil-Felix test

23
Q

are lactose-negative, deaminase (PAD) positive, H2S-negative, and motile

Characterized by a pungent odor

Ferment mannose, and do not swarm on SBA

A

Providencia species

24
Q

– the only urease positive providencia

A

Providencia rettgeri

25
Q

Providencia

have been associated with human infections.
Associated with nosocomial infections of the urinary tract and the skin

A

P.rettgeri and P.stuartii

26
Q

Providencia

– associated with UTI in those with indwelling catheter

A

P. stuartii

27
Q

– only species within the genus Morganella

A

Morganella morganii

28
Q

Citrobacter

Three species most often isolated from diarrheal stool cultures

A

§ Citrobacter koseri
§ Citrobacter braakii
§ Citrobacter freundii

29
Q

– associated with infectious diseases in hospital settings such as UTIs, pneumonias, and intra-abdominal abscess. It is also associated with endocarditis in IV drug abusers

A

Citrobacter freundii

30
Q

General Characteristics
Motile, biochemically resembles Salmonella

A

Citrobacter

31
Q

Differentiated with Salmonella through:
§ Urea hydrolysis – 70% of C.freundii___
Salmonella are___

§ LDC - all species of Citrobacter are___

A

(+); negative

negative

32
Q

Edwardsiella
General Characteristics

Composed of three species:

A

E. tarda; E. hoshinae; E. ictaluri

33
Q

§ Isolated from cold blooded animals
including reptiles, freshwater and
aquarium fish, frogs, and turtles.

A

E. tarda; E. hoshinae; E. ictaluri

34
Q

Edwardsiella

– the only recognized pathogen.
§ Opportunistic
§ Isolated in GI infections

A

E.tarda

35
Q

Edwardsiella

All of them are____ for urea
hydrolysis
All of them are____ for:
§ Lysine decarboxylase
§ H2S, and indole

All of them do not grow on____

A

negative

positive

Simmon’s
citrata

36
Q

Plesiomonas
General Characteristics
Formerly under the family…

A

Vibrionaceae

37
Q

Oxidase-positive (take note that Enterobacteriaceae are oxidase negative!), glucose-fermenting,
facultatively anaerobic, gram-negative bacilli

Phylogenetically closely related to Enterobacteriaceae, but it does not have the ability to produce
gas from glucose

Only oxidase-positive member of the family Enterobacteriaceae

A

Plesiomonas

38
Q

Pleiiomonas

– the only species in this genus.

A

Plesiomonas shigelloides

39
Q

– appear to have much lower virulence
compared to Shigella spp.

It has emerged as a potential cause of
enteric disease in humans, most often after consumption of
undercooked seafood or untreated water.

A

Plesiomonas shigelloides

Bacte LEC (51 decks)

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