Nan Fermenting GNB Prt. 2

Question 1

→ 1950:______
→ previously part of Pseudomonas

Answer 1

Burkholderia

Walter H. Burkholder

Question 2

Burkholderia

→ UBIQUITOUS GNB; OBLIGATE AEROBES MOTILE (EXCEPT:______)

Answer 2

B. mallei

Question 3

→ agent of GLANDERS DSE

Answer 3

B. mallei

Question 4

→ agent of MELIOIDOSIS

Answer 4

B. pseudomallei

Question 5

→ PULMONARY INFECTION (CF)

Answer 5

В. cepacia

Question 6

→ Causes Glander’s disease (Horses to human
transmission)
→ Rare human transmission

Answer 6

Burkholderia mallei

Question 7

Burkholderia mallei

Characteristic
Description
Motility

polymyxin B

oxidase

Temperature

BAP

Answer 7

NON-MOTILE

RESISTANT

WEAK POSITIVE

FAILS TO GROW AT 42°C

SMOOTH, CREAM TO WHITE

Question 8

→ agent of MELIOIDOSIS OR WHITMORE’S DISEASE
• Aggressive granulomatous pulmonary
disease → lead to septicemia

Answer 8

Burkholderia pseudomallei

Question 9

• Also known as VIETNAMESE TIMEBOMB
→ takes years to manifest

Answer 9

Burkholderia pseudomallei

Question 10

Burkholderia pseudomallei

Characteristic
Description
SBAP

ASHDOWN MEDIUM

Oxidase

Motility

ARGININE

OF MEDIUM

Answer 10

WRINKLED. CREAM-TAN COLONIES

DEEP PINK COLONIES WITH EARTHY ODOR

POSITIVE

MOTILE

POSITIVE

OXIDIZES GLUCOSE, MALTOSE, LACTOSE, & CELLOBIOSE(unique)

Question 11

COMPONENTS OF ASHDOWN MEDIUM

_____________
_____Making it a selective medium
→
→

Answer 11

Crystal violet & gentamycin

gentamycin

4% glycerol
Neutral red

Question 12

Cause of foot rot in humans Opportunistic & nosocomial

Answer 12

Burkholderia cepacia

Question 13

Burkholderia cepacia

Characteristic
Description
Motility

Colony

Oxidase

Arginine

Lysine

OF Medium

TREATMENT

Answer 13

MOTILE WITH POLAR FLAGELLA

YELLOW, SERRATED

WEAKLY OXIDASE POSITIVE

NEGATIVE

POSITIVE

OXIDIZES GLUCOSE, MALTOSE, LACTOSE & MANNITOL

sulfamethoxazole, chloramphenicol,
or 3rd gen cepahlosporins

Question 14

➔ COLONIZE: respiratory, urinary, genitourinary tracts
➔ NOSOCOMIAL INFECTION: pneumonia, septicemia,
UTI, & meningitis

Answer 14

Stenotrophomonas (Xanthomonas) maltophilia

Question 15

Stenotrophomonas (Xanthomonas) maltophilia

Characteristic Description

Motility

Tsa

Pyocyanin & Pyoverdin

Odor

Sba

O-F

Oxidase

Dnase

Nitrate

TREATMENT Susceptible to

Answer 15

MOTILE

LARGE, SMOOTH, GLISTENING
COLONIES WITH A YELLOW TO
TAN PIGMENT

ABSENT

AMMONIA-LIKE ODOR

LAVANDER-GREEN TO LIGHT
PURPLE PIGMENT; GROW AT 42 C

Medium WEAK OXIDIZER OF GLUCOSE, STRONG (MALTOSE)

NEGATIVE

POSITIVE

NOT REDUCED

trimethoprim-sulfamethoxazole

Question 16

Acinetobacter
➔ 2 most common human pathogen:

Answer 16

● A. baumannii
● A. lwoffii (formerly known as A.
calcoaceticus var. lwofii)

Question 17

Acinetobacter
➔ Nosocomial & opportunistic pathogen
➔ Infection: UTI, RTI, wound infxn, bacteremia, & meningitis

➔ “Mima” → mimic appearance of_____ (Do Oxidase Test)

Answer 17

Neisseria

Question 18

Acinetobacter

Characteristic Description
Oxygen
Oxidase
Catalase
Motility

Penicillin

Answer 18

OBLIGATE AEROBES

NEGATIVE (TO DIFFERENTIATE)

POSITIVE

NON-MOTILE COCCOBACILLI
Culture Media GROWS ON EMB, MAC & SBA

RESISTANT

Question 19

DIFFERENTIATION OF ACINETOBACTER SPECIES

Lysine decarboxylase

Answer 19

baumannii CHO utilization

Question 20

Lactose assimilation

A. baumannii
A. lwofillii

Answer 20

Rapid assimilation
Negative

Question 21

A. baumanni
A. lwoffii

Fluorescence-Lactose Denitrification (FLN) Acid

Answer 21

Positive
Negative

Question 22

➔ Tiny GNB
➔ Motile

Answer 22

Alcaligenes

Question 23

➔ A. faecalis → CLINICALLY SIGNIFICANT
● Odor: Fruity; apples and pears
● Opportunistic infxn

Answer 23

Alcaligenes

Question 24

Alcaligenes

Characteristic Description

Oxidase
Catalase
SBA

Answer 24

Culture media Grows on MacConkey

POSITIVE

POSITIVE

Flat, dull colonies w/ irregular margin