Nan Fermenting GNB Prt. 2 Flashcards
→ 1950:______
→ previously part of Pseudomonas
Burkholderia
Walter H. Burkholder
Burkholderia
→ UBIQUITOUS GNB; OBLIGATE AEROBES MOTILE (EXCEPT:______)
B. mallei
→ agent of GLANDERS DSE
B. mallei
→ agent of MELIOIDOSIS
B. pseudomallei
→ PULMONARY INFECTION (CF)
В. cepacia
→ Causes Glander’s disease (Horses to human
transmission)
→ Rare human transmission
Burkholderia mallei
Burkholderia mallei
Characteristic
Description
Motility
polymyxin B
oxidase
Temperature
BAP
NON-MOTILE
RESISTANT
WEAK POSITIVE
FAILS TO GROW AT 42°C
SMOOTH, CREAM TO WHITE
→ agent of MELIOIDOSIS OR WHITMORE’S DISEASE
• Aggressive granulomatous pulmonary
disease → lead to septicemia
Burkholderia pseudomallei
• Also known as VIETNAMESE TIMEBOMB
→ takes years to manifest
Burkholderia pseudomallei
Burkholderia pseudomallei
Characteristic
Description
SBAP
ASHDOWN MEDIUM
Oxidase
Motility
ARGININE
OF MEDIUM
WRINKLED. CREAM-TAN COLONIES
DEEP PINK COLONIES WITH EARTHY ODOR
POSITIVE
MOTILE
POSITIVE
OXIDIZES GLUCOSE, MALTOSE, LACTOSE, & CELLOBIOSE(unique)
COMPONENTS OF ASHDOWN MEDIUM
_____________
_____Making it a selective medium
→
→
Crystal violet & gentamycin
gentamycin
4% glycerol
Neutral red
Cause of foot rot in humans Opportunistic & nosocomial
Burkholderia cepacia
Burkholderia cepacia
Characteristic
Description
Motility
Colony
Oxidase
Arginine
Lysine
OF Medium
TREATMENT
MOTILE WITH POLAR FLAGELLA
YELLOW, SERRATED
WEAKLY OXIDASE POSITIVE
NEGATIVE
POSITIVE
OXIDIZES GLUCOSE, MALTOSE, LACTOSE & MANNITOL
sulfamethoxazole, chloramphenicol,
or 3rd gen cepahlosporins
➔ COLONIZE: respiratory, urinary, genitourinary tracts
➔ NOSOCOMIAL INFECTION: pneumonia, septicemia,
UTI, & meningitis
Stenotrophomonas (Xanthomonas) maltophilia
Stenotrophomonas (Xanthomonas) maltophilia
Characteristic Description
Motility
Tsa
Pyocyanin & Pyoverdin
Odor
Sba
O-F
Oxidase
Dnase
Nitrate
TREATMENT Susceptible to
MOTILE
LARGE, SMOOTH, GLISTENING
COLONIES WITH A YELLOW TO
TAN PIGMENT
ABSENT
AMMONIA-LIKE ODOR
LAVANDER-GREEN TO LIGHT
PURPLE PIGMENT; GROW AT 42 C
Medium WEAK OXIDIZER OF GLUCOSE, STRONG (MALTOSE)
NEGATIVE
POSITIVE
NOT REDUCED
trimethoprim-sulfamethoxazole
Acinetobacter
➔ 2 most common human pathogen:
● A. baumannii
● A. lwoffii (formerly known as A.
calcoaceticus var. lwofii)
Acinetobacter
➔ Nosocomial & opportunistic pathogen
➔ Infection: UTI, RTI, wound infxn, bacteremia, & meningitis
➔ “Mima” → mimic appearance of_____ (Do Oxidase Test)
Neisseria
Acinetobacter
Characteristic Description
Oxygen
Oxidase
Catalase
Motility
Penicillin
OBLIGATE AEROBES
NEGATIVE (TO DIFFERENTIATE)
POSITIVE
NON-MOTILE COCCOBACILLI
Culture Media GROWS ON EMB, MAC & SBA
RESISTANT
DIFFERENTIATION OF ACINETOBACTER SPECIES
Lysine decarboxylase
baumannii CHO utilization
Lactose assimilation
A. baumannii
A. lwofillii
Rapid assimilation
Negative
A. baumanni
A. lwoffii
Fluorescence-Lactose Denitrification (FLN) Acid
Positive
Negative
➔ Tiny GNB
➔ Motile
Alcaligenes
➔ A. faecalis → CLINICALLY SIGNIFICANT
● Odor: Fruity; apples and pears
● Opportunistic infxn
Alcaligenes
Alcaligenes
Characteristic Description
Oxidase
Catalase
SBA
Culture media Grows on MacConkey
POSITIVE
POSITIVE
Flat, dull colonies w/ irregular margin
