Moraxella

Question 1

Moraxelleceae

Members of the Family

Answer 1

• Moraxella
• Acinetobacter
• Pyschrobacter

Question 2

Note:- only isolated in humans and a commensal of the upper tract!

Answer 2

Moraxella catarrhalis

Question 3

: Disease Association
• Opportunistic pathogen
• Upper RT infection in healthy adults and children
• May cause lower RT infection on persons with COPD

Answer 3

Moraxella catarrhalis

Question 4

3rd most common cause of____ and ____ in children

May cause life threatening systemic diseases

Answer 4

acute otitis media and sinusitis

Question 5

Presence of…: indicate possible infections caused by this organism (catarrhalish

Answer 5

intracellular, gram negative diplococci in RT specimens

Question 6

T or F

Catarrhalis
Positive for beta lactamase!

Answer 6

True

Question 7

Moraxella catarrhalis: Laboratory Diagnosis
Specimen Collection

Answer 7

• Collected from middle ear effusic
• Nasopharnyx sinus aspirates
• Sputum aspirates
• Bronchial aspirates

Question 8

Catarrhalis
• Inhibited by_____ present in the media (Resistant strains may grow)

Answer 8

Colistin

Question 9

Catarrhalis

Tolerates lower temperature and grows well at____

Answer 9

28°C

Question 10

Older colonies - “______” appearance

Answer 10

wagon wheel

Question 11

Moraxella catarrhalis: Laboratory Diagnosis

Colonies described as “______” - remains intact when pushed across the plate with a loop (movable colonies)

Answer 11

hockey puck

Question 12

Catarrhalis

Grows on____

Describe colonies

Answer 12

BAP or CAP

Smooth, opaque, gray to white

Question 13

Catarrhalis

Oxidase
Catalase

Answer 13

OXIDASE POSITIVE; CATALASE POSITIVE

CPOP

Question 14

Catarrhalis

Sugar utilization

Answer 14

Asaccharolytic - cannot utilize carbohydrates aerobically or anaerobically.

Question 15

Tests to Differentiate Moraxella from Niesseria

Answer 15

Grows on NA
DNAse positive
Fails to utilize CHO in the CTA medium
Positive for butyrate esterase reaction (Tributyrin as the substrate)