1b

Question 1

• distinguishes Klebsiella spp., from other members of the family Enterobacteriaceae

Answer 1

Absence of motility

Question 2

Usually found in the G.I tract; free living in soil, water and plants

All are possess a polysaccharide capsule (encapsulated)

Answer 2

Klebsiella

Question 3

Kblebsiella
General Characteristics
Capable of utilizing_____ as carbon source and can grow in____

Answer 3

citrate

KCN broth

Question 4

Klebsiella

Do not produce indole (except_____!)

They possess______ antigens

Urease___ and ornithine decarboxylase___

Answer 4

K. oyxtoca

O and K

(+)

(-)

Question 5

Klebsiella

Unable to produce H,S

All are lactose fermenter except (2)

Further ferments glucose via the VP pathway

Answer 5

K. ozaenae and K. rhinoscleromatis

Question 6

Has a polysaccharide capsule - DISTINCT
FEATURE

• Confers phagocytosis protection and against antimicrobial absorption.

• Responsible for the moist, MUCOID, colonies

• Also tests positive for Neufeld Quellung test (capsular swelling)

Answer 6

Kblebsiella pneumoniae

Question 7

(Friedlander’s bacilli)

Answer 7

Kblebsiella pneumoniae

Question 8

Disease Association
• Community - Acquired Pneumonia
(CAP), nosocomial

• Opportunistic infections newborns, elderly patients, among and seriously ill patients.

• Wound infections, UTI
and
bacteraemia

Answer 8

Kblebsiella pneumoniae

Question 9

is an important cause of ventilator-associated pnuemonia.

Answer 9

Carbapenemase - producing K.pneumoniae

Question 10

Klebsiella

• - these are versatile Beta-lactamases
that can hydrolyze penicillins, cephalosporins, monobactams, and carbapenems

• Renders many beta lactam drugs inactive

Answer 10

Carbapenemase

Question 11

Laboratory Diagnosis
Gram stain - Gram negative bacilli

Culture - large, moist, mucoid colonies on EMB, MacConkey and XLD

Answer 11

Kblebsiella pneumoniae (Friedlander’s bacilli)

Question 12

Growth in KCN using Moeller KCN Broth Base
• Supplemented with potassium cyanide, is useful in differentiating organisms based on their ability to grow in the presence of cyanide.

• Used for the differentiation of the members of Enterobacteriacee on the basis of potassium cyanide tolerance.
Test is positive if there is marked turbidity in the tube. In negative tests, there is no growth.

Answer 12

Kblebsiella pneumoniae (Friedlander’s bacilli)

Question 13

Bacteria that tolerate KCN -

Bacteria that are inhibited by KCN -

Answer 13

Proteus, Klebsiella, Citrobacter

Salmonella, Shigella, Escherichia

Question 14

Causative agent of antibiotic-associated hemorrhagic colitis (AAHC).

Answer 14

Kblebsiella oxytoca

Question 15

• Observed.
during
therapy
amoxicillin, penicillins, or ampicillin.
with amoxicillin-clavulanate,
• Sudden onset of bloody diarrhea a after 2 to 7 days of treatment with the oral antibiotics (mentioned in the previous bullet).

Answer 15

Kblebsiella oxytoca

Question 16

• Antibiotic therapy disrupts the normal gut microbiota and promotes
the overgrowth of Klebsiella oxytoca with subsequent production of cytotoxin
called tilivallin that mitotic arrestotI host cells and induces apoptosis.

• resolves spontaneously within 2-3 days when the offending antibiotics are discontinued.

Answer 16

antibiotic-associated hemorrhagic colitis (AAHC)

Question 17

Formerly called Donovania granulomatis then Calymmatobacterium granulomatis before being reclassified as

Answer 17

Klebsiella granulomatis

Question 18

§ Causes granuloma inguinale – a granulomatous
disease affecting the genitalia and inguinal area

Answer 18

Klebsiella granulomatis

Question 19

Disease is called donovanosis

§ Transmitted through unprotected sexual intercourse

§ After prolonged incubation of weeks to months, subcutaneous nodules appear on the genitalia or in the genital area

Answer 19

K granulomatis

Question 20

Kblebsiella granulomatis

are rod-shaped, oval organisms that can be seen in the cytoplasm of mononuclear phagocytes or histiocytes in tissue samples from patients with
granuloma inguinale.

They appear deep purple when
stained with Wright’s stain.

Answer 20

Donovan bodies

Question 21

Causes Rhinoscleroma a chronic
granulomatous disease

Answer 21

Kblebsiella rhinoscleromatis

Question 22

commonly affects the nasal cavity
and nasopharynx, but it can also
involve the larynx, trachea, bronchi,
middle ear, and orbit.

Acquired via inhalation of
respiratory droplets containing
the causative agents

Answer 22

Kblebsiella rhinoscleromatis

Question 23

Kblebsiella rhinoscleromatis

Diagnosis of rhinoscleromatis
depends on the identification of the
pathognomonic_______ which is most prominent during granulomatous phase.

Answer 23

Mickulicz cells
(MCs)

Question 24

foamy macrophages with numerous
cytoplasmic vacuoles containing
viable and nonviable Klebsiella bacilli

Answer 24

Mickulicz cells (MCs)-

Question 25

Causative agent of chronic atrophic rhinitis called ozena.

Answer 25

Kblebsiella ozanae

Question 26

is a disease of the nose in which the bony ridges and mucous membranes of the nose waste away

Transmitted via person-to-person contact

Answer 26

Ozena