Rhizoctonia solani

Question 1

Rhizoctonia solani

Answer 1

is a ubiquitous soil-born plant funfi

Question 2

where is it distributed

Answer 2

worldwide and wide host range

Question 3

why is it difficult to control using chemicl

Answer 3

• soil dwelling
• widespread resistance to fungicides
• soil fumigant are banned

Question 4

most effective way to prevent the disease is to

Answer 4

evade te pathogen altogether e.g. test soils for infestation prior to sowing with susceptible crops

Question 5

R.solani speciifc mAbs

Answer 5

monoclonal antibodies raised against a surface glycoprotein antigen

Question 6

name protein epitope

Answer 6

mAb EH2

Question 7

name a carbohydrate epitope

Answer 7

mAB EE1

Question 8

antigen is only secreted by

Answer 8

live cells

Question 9

what can be used to visualise R.solani hyphae

Answer 9

immunofluorescence

- create a monoclonal Ab specific to R.solani (mAb EH2 and EE1) and tag with fluorescent protein

Question 10

diagnostic procedure

Answer 10

1) stimulate active grow using baits
2) detect R.solani using specific mAbs
3) Recover positive isolates for further testing

Question 11

which ELISA can be used

Answer 11

DAS-ELISA

Question 12

DAS-ELISA

Answer 12

double antibody sandwich, enzyme linked immunosorbent assay

Question 13

what are isolated from the bait for the ELISA

Answer 13

secretion of target antigen from fungus on seed bait

Question 14

how long does the assay take

Answer 14

24h

Question 15

24h

Answer 15

very long time