Point of care diagnostics- Lateral flow technology Flashcards
in lateral flow assay for the diagnosis of IPA which antibody is used
JF5 Ab
J5F Ab is labeled with
colloidal gold- shows up red
when antigen is present (person with IPA)
antigens bind to the labeled J5F Ab (comes before test and control line).
- these labeled J5F Ab flow up the pad via capillary action onto the membrane and binds to the immobile J5Ab
- unbound labeled antibodies will bind to the control antibodies (double line)
when antigen is not present
labeled J5F Ab bind to the control immobilised antibodies alone (single line)
outline protocol for how sample is prepared to be used in LFA
serum or haemorrhage BAlL combined with sample buffer and heated to 100 degrees
- centrifuged
- liquid at top added to LFA
Lateral flow assay for IPA detects what
J5F antigen in human sera
bronchoalveolar lavage used to
isolate aspergillus fumigateurs antigens
how does BAL occur
bronchoscope put down trachea and into the left primary bronchus- water flushed and then sucked back up
Non-invasive molecular imaging is
antibody guided
antibody guided molecular imaging uses which machinery
ImmunoPET/MRI
lung diseases
all look quite similar on CT scan
on an x ray
- On an x-ray typically see diffuse lung patterning
radiologists look for
abnormalities in the lungs
radiological tests are very
- Radiologists looks for abnormalities in the lungs
- Very non-specific for fungal infections
- E.g. cant tell you directly what it is- can just tell that something is not right
antibodies can be used to
show the presence of IPA in the lungs- very specific
