Diversity Of Cells And Their Function Flashcards
What are the 3 primary histology techniques
Tissue processing
Embedding and sectioning
Staining - colour different parts of the cell
What are the stages of tissue processing (general)
Sample removed
Fixation
Dehydration
Clearing
Infiltration
What happens during the ‘fixation’ stage of tissue processing
Sample soaked in fixated solution - acetic acid
What happens during the ‘dehydration’ stage of tissue processing
Using high concentrations of ethyl alcohol the water in the tissue is replaced with alcohol
What happens during the ‘clearing’ stage of tissue processing
Clearing agent such as xylene is used to replace the soluble alcohol
What happens during the ‘infiltration’ stage of tissue processing
Non-soluble xylene is replaced with non-soluble paraffin wax which surrounds the sample
Which tissue components take up acidic stains
Acidophilic
What colour does acid stain
Red or pink
Eosin
What tissue components take up basic stains
Basophilic
What colour does basic stain
Blue or purple
Hematoxylin
What colour does neutral stain (nt. charge)
Give different colour to different components
What does hematoxylin bind to
DNA/RNA and colours the nucleus
What does Eosin bind to
Amino acids/proteins therefore colours cytoplasm pink
What are the 4 tissue types
Epithelium
Connective tissue
Muscle tissue
Nervous tissue
What is the function of epithelial tissue
Line hollow organs
Form glands
What is the function of connective tissue
Forms framework
Fills gaps - transport of nutrients
How are the cells of muscle tissues specialised to contract
Contain more cytoskeleton
What is the function of muscle tissue
Specialised to contract
Have more cytoskeleton to contract
What are the 3 types of muscle tissue
Cardiac
Skeletal
Smooth
What does nervous tissue consist of
Neurons
supporting cells
What is the function of nervous tissue
Specialised to conduct impulses
What are the characteristics of epithelia
Polar
Tightly adherent
Avascular
What are the types of epithelium
Covering epithelium
Glandular epithelium
What is a simple epithelium
Single cell layer
What is a stratified epithelium
2 or more cell layers
What is a pseudo-stratified epithelium
Single cell layer, appears as multiple cell layers as nucleus are at different levels