Medicine Sem 1 > Cytology week3,4,5 > Flashcards

Cytology week3,4,5 Flashcards

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1
Q

Name the 4 hyrolitiic enzymes in lysosomes

A
  • Nucleases
  • Proteases
  • Phospholipases
  • Phosphates
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2
Q

Explain the formation of lysosomes

A
  • Lysosmal hydrolases is synthesied in the rER then transferred to the golgi apparatus where it is modified with M6P for lysosmal targetting
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3
Q

What are lysosomes responsible for and what do they contain

A
  • Intacellular digestion
  • recycling cellular components
    They contain around 40 hydrolitic enzymes that break down macromolecules
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4
Q

Explain the process in the digestion of external materials

A

-The membrane of the phagosome vesicle fuses with the lysosomes.
- This mixes the endocytosed materials with the lysosomal emzyme activating proton pumps in the lysosmal membrane, allowing digestion and forming a secondary lysosome(heterolysosomes)

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5
Q

What happens in Autophagy

A

-Lysosomes degrade excess or defective cells through autophagy.
-Autophagosomes form around unwanted cellular componentes and fuse with lysosomes for digestion.

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6
Q

What do indigestible materials form

A

They form residul bodies which can accumulate as lipofusion in long live cells(heart muscle and neurons)

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7
Q

Describe the structure and size of proteasomes and where they are found

A
  • Large non membrane bound protein complexes
  • Located in the cytoplasm and nucleus
  • They consist of a cylindrical structure with 4 stacked rings
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8
Q

What is the difference between proteasomes and lysosomes

A

Proteasomes target indiviudal proteins whereas lysosomes digest organelles or membranes by autophagy

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9
Q

What is the role of mitochondria?

A

It is responsible for aerobic respiration, ATP production, providing energy for cellular activities
- They oxidise pyruvate releasing more ATP than gylcolysis

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10
Q

Describe the structure of mitochondria

A
  • Elongated, highly dynamic
  • Has 2 membranes, an outer sieve like membrane, and a folded inner membrane called cristae, which increases the surface area and house key enzymes for energy production
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11
Q

Describe the inner membrane of the mitochondria

A
  • It contains specialised phospolipds
  • It is highly impermeable to ions, hosts transport proteins
    and the electron transport chain and ATP synthase for oxidative phosphorylation
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12
Q

What is the role of proteasomes

A

They degrade damaged or unecessary proteins

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13
Q

How is ATP produced in the mitochondria

A

-Electrons from the ETC create a proton gradient across the inner membrane and then protons flow back through ATP synthase, generating ATP.

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14
Q

What is mitochondrias role in Apoptosis?

A

During cell stress, mitochondria release cytochrome C, triggering apoptosis by activating cellular proteases

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15
Q

What is the function of peroxisomes?

A
  • They produce and degrade hydrogen peroxide through oxidase and peroxidase
  • They detoxify harmful substances, especially in the liver and kidney.
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16
Q

What is the role of peroxisomes in metabolism

A

It breaks down long fatty acid chains and synthesising bile acids and cholesterol

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17
Q

How are peroxsisomes formed

A
  • By budding from the ER or by growth and divison of existing peroxisomes.
  • Their proteins are synthesised in the cytosol and imported using targeting sequences
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18
Q

What does cytoskeleton consist of?

A
  • Microtubules
  • Micro filaments(Actin filaments)
  • Intermediate filaments
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19
Q

What is the function of Cytoskeleton

A
  • It provides structural supprt
  • Determines the cell shape
  • Enables movement of organelles and vesicles
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20
Q

Explain the structure of Microtubules

A
  • Hollow rigid tubular structures
  • Made of alpha and beta tubulin subunits
  • Linked side by side in structures like cilia
  • Highly dynamic
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21
Q

What is the microtubules function in intracelluler transports

A
  • It facilitates vesicle,organelles and complex transport
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22
Q

What is the microtubules function in cell organization?

A
  • They extend the ER and assist vesicle movement through Golgi apparatus
  • They maintain cell shape and polarity
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23
Q

What is the cell cycle

A

Cycles of growth and division

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24
Q

NAME the phases of the cell cycle

A
  • G1 Phase
    -S phase
    -G2 phase
  • Mitosis
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25
Explain the 4 phases in the cell cycle
G1 PHASE - Where active RNA and protein synthesis occurs, increasing cell volume S PHASE - DNA replication, histone synthesis and centrosome duplication happens G2 PHASE - Proteins required for mitosis accumulate MITOSIS - Cells divide, completing the cycle
26
What is the role of Cyclins and CDK(
-They regulate the cell cycle by activating phase specific proteins, - Whe each phase is complete, the cyclin is removed and a new cyclin guides the cell into the next phase
27
What is the role of checkpoints and tummor supressor proteins
- Specific checkpoints monitor conditions and DNA integrity pausing the cycle if issues arrises - Tumour supressor proteins such as p53 help stop the cycle for repair or tigger cell apoptosis if damages are irreperable.
28
Explain what happend in the G0 phase
- Differentiated cells enter the G0 phase, where the cell cycle is apsued - Cells like liver cells can re-enter the cycle , while cells like the mucle and nerve cells stay in the G0 phase permanently. - External signals can re-activate G0 cells
29
Explain the structure of the microfilaments
-Made out of actin subunits and they are - Thin -Flexible -Highly dynamic and they have polarised filaments that enable cell motility and contraction
30
What is Mitosis?
It is the process of cell division where a parent cell divides to produce 2 daughter cells with identical sets of chromosomes.
31
What is the process of prophase
- Chromosomes condense becoming visible chromosomes, each w 2 chromatids joined at the centromere . - The Nucleolus disappears and the centromere migrate to oppiste poles forming the mitotic spindle -The nuclear envelop disassemebels
32
What is the process of Metaphase
- Chromosome fully condenses and aligns at the equatorial plate - Kinetchores on chromosomes attatch to spindle microtubules
33
What is the process of Anaphase
- Sister chromtids seperate and move to opposite spindle poles, now they are considered as individual chromosomes - The spindle pores move further apart, aiding chromosome sepearation
34
What happens in Telopahse
-The chromosomes at the spindle poles decondense -The nuclear envelope re assembles around each chromsom set
35
What are stem cells
A small population of undifferentiated cells in the tissues
36
What is the role of stem cells
- They enable the renewal of differentiated cells through an individuals life
37
Where are stem cells located
-In specifc microevironments , or niches that maintain their undifferentiate state
38
Where are the stem cells studied
In rapidly renewing tissues, such as blood, skin and the digestive tract
39
What is meiosis and what does it involve
- Cell division process that prduces halpoid gametes(sperm+egg) from diploid percursor cell - It involves 2 cell divisions, resulting in 4 geneticically unique haploid cells
40
Explain the key feature of meisois: - Synapsis + crossing over
SYNAPSIS + CROSSING OVER - Where the homologus chromosomes pair up in a process called synapsis, allows for crossovers to occur - The mixing of the genetic material produces new gene combinations, increasing the genetic diversity
41
Explain the the key feature - Haploid cells in meiosis
- This is the result of meiosis with half of the usual chromosome number - This then combines during fertilisation to form a new dilpid zygote
42
What stages of Meiosis
- Prophase 1 - First Meiotic division - Second Meiotic Division
43
What happens in the first stage of prohpase 1
-chromosome condesne , pair up , and undergo sypnapsis - Crossing over occurs, promoting genetic variation
44
What happens in the first meiotic division?
- Homologus chromosomes seperate into new cells, each are still diploids but with paired chromatids -
45
What happens in the third stage of meiosis
- Chromosomes seperate without DNA replication , forming halpoid cells with single chromatids each
46
What is the difference between Meiosis and Mitosis
Mitosis: - Produces 2 genetically identical diploid cells - No genetic variaton( no crossing over or recombination) Meiosis: Produces 4 genetically different/unique halpoid cell
47
Which components are in the Nucleus?
- Nuclear envelope -Chromatin - Nucleoli
48
1.What is the Nuclear envelope? 2. What is the structure of the Nuclear envelope?
1- It is a selectively permeable membrane that acts as a boundary between the nucleus and cytoplasm 2- Has 2 membranes, separated by a perinuclear space
49
What is the inner membrane of the nuclear envelope supported by ?
- Nuclear lamina which is a protein network
50
What is Apoptosis
- hIghly regulated process of programmed cell division
51
What is the role of Apoptosis
It eliminated defective and unneeded cells without causing inflammation
52
What is the mechanism of Apoptosis
It is controlled by Bcl-2proteins - The proteins regulate the release of death-promoting factors from mitochondria - They are activated by external signals or irreversibke internal damage
53
Name the four basic cell types
-Epithelial tissue -Connective tissue - Muscle tissue - Nervous tissue
54
What components do organs consist of and their function?
- Parenchyma: responsible for organs specialised fucntions - Stroma: supports connective tissue
55
Name the 3 functions of the epithelial cell with a brief explanation
- Protection( supports and protects the surfaces) - Absorption( Faciliatates nutrient uptake) - Secretion(produces and releases substances)
56
What are three shapes epithelial cells can have?
Columnar Cuboidal Squamous
57
Explain the shape of the nuclei in the columnar, cuboidal or squamous cells
Elongated in columnar cells Flattened in squamous cells Spherical in cuboidal cells
58
What is the function of the basal pole and the apical pole?
Basal pole: Contracts ECM and the connective tissue Apical pole::Faces an open space
59
Explain epithelial nutrient supply
-Epithelial lack blood vesselsand depend on on nutrients and oxygen from adjacent connective tissues
60
What is Laminate propria.
Connective tissues underlying the epithelia
61
What is the function of the basment membrane in the Epthelial cells
- Provides structural support - Maintains epithelial polarity - Facilitates epithelial repair - Regulates molecular filtration
62
What is the basment membrane
- A thin extracellular sheet supporting the epithelial cells - Acts as a semipermeable filter for substances
63
How does the basment membrane look in the TEM
- It is visible under 2 layers. - basal lamina - Reticular Lamina
64
Explain tight (occluding) junctions + what they contain
- Located at the apical end forming a seal between cells to prevent the flow of materials between epithelial cells. -Prevent paracellular transport composed of claudin and occluding proteins
65
What are the functions of the tight junctions
- Prevent paracellular transport - Form a seal between cells to prevent the flow of materials between epithelial cells
66
What are Zonula Adheren junctions + what are they mediated by
-Encircle cells below tight junctions - Cadherins
67
What is the function of Adherene junctions
-Provides mechanical strength and links to a cytoskeleton network
68
What is the function of desmosomes junctions What are desmosomes composed of
Provide firm adhesion and resilience against mechanical stress -Desmoglein, desmocollins, desmoplakins
69
What do gap junctions consisit of
- Aggregated transmembrane protein complexes that form circular patches in the plasma membrane -
70
What is the structure and function of microvilli?
- Small uniform cytoplasmic projections Covered by glycoalyx containing enzymes for digestion
71
What does microvilli contain(components)
Contains bounded actin filaments , stabilised by actin-binding proteins
72
What is the structure of stereocilia?
- It is longer and less motile than microvilli
73
Where are stereocilia found?
- In male reproductive systems and the inner ear
74
What are the components of stereocilia?
Have actin filaments connected to the terminal web
75
What is the function of the cilia( motile and non motile)
Motile:Generates fluid movement along the epithelial surface Non motile: detect environmental signals
76
Where is the flagella found?
- Found in sperm cells
77
What is the function of the flagella
Enables movement
78
What dose chromatin consist of
- DNA and associated proteins
79
What is the function of Nuclear pores?
Facilitate the movement of ion, molecules and macromolecules
80
Name the types of Chromatin
- Eurochromatin -Heterochromatin
81
Explain heterochromatin
- Dense, transcriptinally inactive includes: - Constitutive heterochromatin - Facultative heterochromatin
82
Explain eurochromatin
- Lightly stained , transcriptionally active
83
Cells with more eurochromatin are ……
Metabolically active
84
Cells with more hetero eurochromatin are……..
Less active
85
what is it called when the basement membrane of the epithelial cell has one cell layer and describe the cells shape
Simple cells off simple epithelia are either squamos, cuboidal, or columnar
86
What is epithelia called with 2 or more layers of cells and the shape
- Stratified Mostly all of the stratified epithelia are stratified squamos
87
What is the function of the stratified squamos epithelia
- Move from the basal to the surface layers - become filled with keratin intermediate filaments
88
Name an exmaple of stratified squamos epithelia and its function
- Epidermis - Covers the body surfaces - protects the underlying tissues from excess water loss
89
What is the functions of Macrophages?
-Specialize in phagocytosis -Secrete growth factors critical for tissue repair and regeneration -Play a role in antigen processing and presentation for lymphocyte activation. -Abundant at sites of inflammation and involved in tissue repair.
90
What are the characteristics of Macrophages: Nucleus, surface and Cellular structure
Nucleus: - Oval or kidney shaped Surface: - Irregular with pleats and protrusions, aiding pinocytotic and phagocytic activity Cellular Structure: - Contains well developed Golgi Apparatus and many lysosomes
91
Where do Macrophages orgiinate from?
-
92
Where do Monocytes form?
-Monocytes form in the yolk sac during early embryonic development and become part of the mononuclear phagocyte system
93
Name the gap junction proteins
Connexins
94
What is the function of Gap junctions
- Permit intracellular exchange of molecules with small dimateres
95
What does hemidesmosomes contain
Integrins
96
What causes blistering
- Abnormal desmosome function
97
What causes food poisoning
- The secretion of the enterotoxin by clostridium perfringes , which binds to claudin molecules of the intestinal cells
98
What is the name of the bacteria involved in gastric ulcers
Helicobacter pylori
99
Explain how helicobacter pylori causes gastric ulcers
Binds to extracellular domains of tight junctions , inserting a protein ino the cells which target ZO-1 and distrupts the signalling
100
Where are microvilli found
- Found in small intestine and retinal proximal tubular cells
101
How does the bc1 proteins carry out their function during apoptosis
- Rlease cytochrome c into the cytoplasm -Where it attahes to the Proteolytic enzymes called caspases - Intial caspases activate a cascade of other caspases , resulting in a protein degeneration throughout the cell
102
What is the outer membrane of the nuclear envelope continous with
Rough endoplasmic reticulum
103
What is the inner membrane of the nuclear envelope supported by
- Nuclear lamina
104
Where is constitutive heterochromatin found and what does it contains
-Found in all cells - Contain repetitive sequences
105
What can facultative heterochromatin switch between
- Active and inactive states
106
What do Nucleoporins regulate transport of
Ribosomal subunits, RNA and transcriptional factors
Medicine Sem 1
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