Breeding for Conservation Flashcards

1
Q

Species Conservation

A

Conservation defined as ‘securing populations of species for the long term’

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2
Q

• In-situ Conservation

A
  • Conservation in natural surroundings
  • Management and monitoring of biodiversity where it is encountered • Integrated approach
  • E.g. Nature reserves, biospheres, national parks
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3
Q

• Ex-situ Conservation

A
  • Conservation away from the natural habitat

* E.g. Zoos, aquariums, wildlife parks, safari parks, gene banks.

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4
Q

Role of Zoos in Conservation

A
  • “Zoos and aquariums are the third largest funder of conservation globally” WAZA 2020.
  • Direct or Indirect conservation
  • Financial
  • Material
  • In-kind contribution to research
  • Ex-situ breeding
  • Captive breeding programmes
  • Education
  • Reintroduction programmes
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5
Q

Legal Requirements of Zoos

A
  • Conservation requirements You must do all of the following: • keep animals in an environment suitable for their species
  • do as much as possible to stop any animals escaping
  • stop pests and vermin getting into the zoo
  • keep records of the zoo’s collection
  • help educate people about biodiversity
  • Zoo operators also need to do at least one of the following: • conservation research or training
  • sharing conservation information
  • breeding wild animals in captivity
  • repopulate an area or reintroduce species into the wild • https://www.gov.uk/guidance/keeping-zoo-animals
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6
Q

Captive Exotic Species

A
  • The majority of captive zoo animals are bred in captivity
  • Purchase of animals from other zoos
  • Transfers and exchanges between zoos • National and International
  • Donations
  • Private or public
  • Taken from the wild (very rare)
  • Orphans, injuries, disease risk, threat of extinction, seized from owners who have acquired illegally
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7
Q

Captive Breeding

A

The process of breeding animals outside of their natural environment in restricted conditions in farms, zoos or other closed facilities.
Purpose in zoos and aquariums:
• Increase captive population numbers of threatened or endangered species • Preservation of captive populations
• Reintroduction programmes
• Release programmes
• Safeguarding of the gene pool
• Produce animals for zoos, aquariums, and other public facilities: • Encourage visitors
• Education
• Financial gain

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8
Q

Aim of Captive Breeding Programmes

A

• To form large, viable populations of species ex situ long-term
• Purpose:
• providing animals for public educational and/or exhibit opportunities
• providing fundraising material
• providing research collections from which to gain basic knowledge of animal biology
and husbandry
• providing demographic and genetic backup to wild populations

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9
Q

Viable Populations

A
  • Demographically stable
  • Adequate animals of breeding age
  • Able to reproduce at the required rate
  • Healthy, well maintained and capable of self-sustaining reproduction • Able to breed when needed
  • Distributed among several institutions to lessen the risks of catastrophic loss
  • Sufficient size to maintain high levels of genetic diversity • To remain healthy and adapt to changing environments
  • 90% diversity for 100 years
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10
Q

Management of Breeding Programmes

A
  • International co-operative breeding programmes
  • Four major associations:
  • WAZA: The World Association of Zoos and Aquariums (WAZA)
  • EAZA: European Association of Zoos and Aquariums
  • BIAZA British and Irish Association of Zoos and Aquariums • AZA: Association of Zoos and Aquariums
  • These associations work together for captive breeding programmes
  • In the UK, captive breeding programmes are facilitated and endorsed by EAZA • EEPs
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11
Q

European Endangered Species Programme

A

OLD MODEL
• Established in 1985
• Intensive population management
• An EEP per species in a breeding programme
• Each EEP has a species coordinator (specialist) assisted by the species committee
• Collect information on the status of all the animals of the species in zoos and aquariums • Producing a studbook
• Perform demographic and genetic analyses
• Produce a plan for the future management of the species
• Make recommendations on
• Which animals should breed
• Which animals should not breed
• Which animals should move and to where

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12
Q

EAZA Ex-Situ Programmes (EEPs)

A

NEW MODEL
• Introduced 2018 (5 year role out)
• Population management programmes for 400 species
• Recommends strategies for the protection of zoo populations based on the specific roles that individual species can play in conservation, research, or education
• Change required due to the wide variety of reasons for species conservation for individual species
• Critically endangered animals
• Animals driven from a specific habitat.
• Public preconceptions about species that hamper conservation and fundraising efforts
• Animals bred for reintroduction programmes
• Animals bred to raise awareness of conservation issues that may not be well understood by the
public or legislators

  • Taxon Advisory Groups (TAG) decide which species are to be managed under an EEP
  • The EEP co-ordinator with the EEP species committee:
  • Is responsible for the day-to-day management of each of the EAZA Ex situ Programmes
  • Will develop, implement and update a Long Term Management Plan (LTMP)
  • Manage the EEP studbook dataset
  • Develop (non-)breeding and transfer recommendations, catering to the needs of EEP holders in alignment with the goals of the population.
  • Publish the studbook and annual reports
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13
Q

EAZA Ex-Situ Programme: Hyena

A
  • A bad name with the public
  • The role of the new hyena EEP
  • To educate the public on hyena social life
  • To undertake research on Hyena social life
  • Breeding conservation within social groups
  • To educate on the role Hyenas play in removing disease carrying carcasses from the savannah.
  • To show how each species contributes to the health of the world’s ecosystems
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14
Q

Studbooks

A
  • Data base for individuals included in a captive breeding programme
  • Primary objective is to facilitate the coordinated management of ex situ populations
  • For each individual the studbooks should include:
  • Identities of sire and dam
  • Date and location of birth
  • Full transaction history (names of owners and dates of ownership changes)
  • Where animals have been obtained from or released into the wild, the studbook should record, if possible, details of the capture or release location
  • Any individual identifiers (e.g. house names, local identification numbers, tags, transponders, tattoos)
  • Any data on the reproductive potential of living animals (e.g. temporary contraception method and dates, permanent contraception such as castration)
  • Date and location of death
  • Cause(s) of death and information on disposal of body.
  • Other information pertinent to a particular programme (e.g. sub-species, genetic analysis).
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15
Q

Studbook Data Systems

A

• From 1989 studbooks typically maintained in the DOS-based Single Population Analysis & Records Keeping System (SPARKS)
• SPARKS was developed by ISIS (International Species Information System)
• Since January 2017, SPARKS has gradually been replaced by ZIMS (Zoological
Information Management System)
• Digital studbook data set developed by Species360 (formally ISIS – International Species Information System)
• ZIMS for studbooks is now a requirement of EAZA for all EEPs

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16
Q

Other Considerations with conservation programs

A

• Social Interaction
• Solitary species, housing, husbandry, appropriately matched animals,
transportation • Genetic goals
• Prevent inbreeding and maintain genetic diversity • Behavioural competence
• The ability of an animal to express appropriate behaviour in a given situation, e.g. courtship, parenting
• An animal that does not show appropriated breeding behaviour may not
like the mate selected

17
Q

Breeding Cycle in Captivity

A
  • Identify breeding pairs
  • Prepare male and female for mating
  • Oestrus detection
  • Mating
  • Natural
  • Artificial insemination
  • Embryo transfer
  • Confirmation of pregnancy
  • Maintenance of pregnancy
  • Care of offspring
18
Q

Natural Mating in Captivity

A

• Easiest least invasive method
• No always part of an EAZA Ex-situ breeding programme
• Is this ethical?
• Highly suited where both male and females of a species exist at one zoo
• Consider genetics and inbreeding
• Individuals may be transported from other zoos once identified via EEP co-
ordinator for breeding
• If long distance this may require quarantine (up to 6 months) • Need to consider housing
• Mimic natural (wild) breeding behaviours in captivity

19
Q

Breeding Cheetahs for Conservation: Preparation

A
  • Cheetahs: a natural mating success story (aided by research and understanding) • Captive populations are small and fragmented
  • Intensive management required to retain genetic diversity
  • Housing
  • Hoofstock in adjacent exhibits can increase reproductive behaviours
  • Predators in adjacent exhibits will reduce reproductive behaviours
  • Social Structure
  • Group structures have greater breeding success
  • Separate males and females physically and visually (reduced likelihood of sibling bond)
  • Rotate animals through enclosures and introduce new animals (increases breeding behaviours) • Logisticsincaptivity
20
Q

Breeding Cheetahs for Conservation: Oestrus Detection

A
  • Induced ovulation (ovulation induced by mating and presence of male)
  • Introduce male to female (close proximity)
  • Eg male in outside enclosure overnight, female indoors
  • Can measure faecal oestrogen concentrations as signs of ovulation
  • Female may
  • Roll on ground, Defaecate on prominent sites such as on trees
  • Males exposed to female faeces and urine (if receptive to male) • Vocalise, Run, Display erections
  • In adjacent enclosures, the male’s reaction normally intensifies and the female normally shows a combination of tail flagging, rolling or rubbing her head against objects
21
Q

Breeding Cheetahs for Conservation: Mating

A
  • Staff must be experienced in recognising behaviours • Reduces fighting risk
  • Helps forms successful breeding pairs
  • May be some aggression
  • Copulation usually occurs with the hour and lasts 30-45 seconds
  • Strong mate preferences in males and females
  • Introduce the female to one male.
  • Introduce the female to a coalition.
  • Introducing the female to a different male each day whilst receptive
  • Introduce the female to a male of choice, by placing different males in adjacent enclosures
22
Q

Breeding Cheetahs for Conservation: Confirmation of Pregnancy

A

• Gestation 90-98 days
• Female separated from group
• Faecal progesterone tests:
• 70 days post-breeding.
• Progesterone levels increase above baseline post-breeding with or without pregnancy until day 60 post-breeding
• Progesterone declines from d60, rising again when the female is pregnant.
• Weekly weighing:
• During (false) pregnancy there is a steady weight gain during the first 60 days.
• After 60 days females having a false pregnancy start to lose weight, whereas pregnant females
will continue to gain
• Ultrasound:
• Requires that females are trained and not stressed by the procedure

23
Q

Maintenance of Pregnancy

A
  • Housing
  • Warmth
  • Place to give birth
  • Shelter
  • Able to separate from other animals
  • Nutrition • Energy
  • Protein • Fats
  • Health
  • Vaccinate
24
Q

Care of offspring

A
  • Wild animals
  • Minimal interaction with humans
  • Monitor for behaviours
  • Monitor for health
  • Colostrum (mammals)
  • Warmth • Care
  • Consider when to separate
25
Q

Further Challenges for Cheetahs

A
  • Many females do not breed in captivity
  • Poor semen quality of males
  • To utilise genetics use artificial insemination or embryo transfer
  • Artificial Insemination
  • Unsuccessful
  • Induced ovulation protocol need refinement • Difficult to inseminate to the uterine horns
  • Embryo transfer via OPU-IVF
  • OPU and IVF successful
  • Difficulties with transfer of embryos • Feb 19th 2020 first ET cubs born
26
Q

Artificial Insemination

A
  • Suitable when limited availability of individuals • Dependent on effective and reliable techniques • Requires expertise and research
  • Requires technical facilities
  • Can be invasive
  • Success with AI seen in captive elephants
27
Q

Artificial Insemination Elephants

A
  • Oestrus Detection • Semen collection • Insemination
  • Few mature male bulls
  • Poor sperm quality and low libido • Reduced transportation of animals • Older females less likely to breed
28
Q

Oestrus Detection Elephant

A
  • Endocrine monitoring techniques • Faecal and urine monitoring
  • LH surge
  • Double LH surge technique
  • LH surge 3 weeks prior to ovulation
  • LH surge 1 day prior to ovulation (ovLH surge)
29
Q

Semen Collection and Storage elephant

A
  • Collected by transrectal electroejaculation of an anaesthetised bull • Semen quality is variable
  • Advise 70% motility for use in AI programme
  • Semen usually chilled
  • Semen can be cryopreserved
  • Tusker Task Force, Semen Biobanking of African Elephant Bulls
  • https://youtu.be/Ls9pzL8igGo
  • Semen collection of wild bull semen for preservation of genetics
30
Q

Artificial Insemination Elephants

A
  • Over 50 individuals born by AI in captivity worldwide
  • First success in 1998
  • Calf born 2000 (22 month gestation)
  • Routine procedure
  • Well-established
31
Q

Conservation Breeding IVF

A
  • Useful where individual numbers are very low • Previously stored semen
  • Collected (stored) oocytes
  • Difficult to develop
  • Requires expertise and technical ability • Research-led
  • An option for “extinct” species
  • The only lifeline for the Northern White Rhino
32
Q

Ethical Considerations Captive Animals

A
  • Good intentions to conserve species
  • But does captivity cause harm?
  • Ethical paradox:
  • Conservation is about the species
  • Animal welfare is about the individual
  • Utilitarian Opinion
  • The greater good for the greatest number
  • Harm to a few individuals is justifiable because it benefits the species
  • Animal Welfare Opinion
  • Immanueal Kant (18th Century)
  • The act itself must be moral and have a pure intention regardless of final consequence
33
Q

Reasons ‘For’ Captive Breeding

A
  • Conservation of the species
  • Conservation of genetics
  • Moral obligation to conserve species • Habitat loss
  • Poaching
  • Safer in zoos than in wild where they would not survive
  • Has prevented extinction in a number of species • Golden Lion tamarin
  • Arabian oryx
  • California condor
  • Partula snails
  • Przewalski’s horse
  • Socorro dove
34
Q

Reasons ‘Against’ Captive Breeding

A
  • Low success of reintroduction programmes
  • Number of captive individuals too low to maintain genetic diversity
  • Benefit to overall species does not outweigh negative effect to individuals
  • Captive breeding not well established in a significant number of species
  • Removal of wild species from their natural habitat for breeding is immoral
  • Many of the animals kept in zoos are not for breeding programmes
  • Only 800 of the 7368 species can be conserved through captive breeding due to space limitations in zoos
35
Q

Ethical Considerations: Breeding

A
  • Which species to breed? • Key species
  • Those most at threat
  • Those that have greatest chance of survival • Successfulreintroduction
  • An integrated approach is required • Reason for decline targeted
  • Habitat preservation
  • In-Situ conservation?
  • Pan-Situ
  • Captive populations used for restocking (where individual exist in-situ) • In-situ populations taken to captivity for genetic diversity