WBC Flashcards
What are automated WBC differential useful clues for
Evidence of and response to infection
Allergic response
Leukaemia
Marrow infiltration
What does flow cytometry show
lymphoid and myeloid populations and degree of cell maturity
What can a bone marrow examination be used for
number and type of abnormal/normal cells
aspirate
needle to remove fluid
trephine
core of bone marrow
neutrophils - horse shoe
-rise as acute response to bacterial infection, inflammation and malignancy
Ingest bacteria kill in granules (lysosomes/peroxidase)
NON-SPECIFIC
8-10 hour lifespan
Chemotaxis
the movement in response to antigens
Eosinophils
4-5 hours growth promoted by interleukin-5 contain major basic protein and reactive O2 species Fight parasitic infection (helminth) Allergies
Chemokines
attract WBC to certain tissues
Basophils
Dark granules contain: -histamine -leukotrienes -proteases -heparin role in phagocytosis role in allergic reaction
What surface receptors do basophils have
IgE surface receptor = binding produces degranulation
Lymphocytes
differentiate from other marrow derived cells
B(one marrow) and T(hymns) cells
If there are circulating T and B cells with malignancies = leukaemia
BIG DARK SPOT
Macrophages
contain hydrolyses and myeloperoxidase
ingest and present to T cells
Kill microbacteria and fungi
CML
All ages anaemia, large spleen, bone pain high WBC and platelet chronic - blast phase identical cytogenetic molecules mutation so treatment well tolerated
AML
more common with increasing age symptoms of marrow failure anaemia bleeding infections (sepsis)
