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Microbiology -Medical > Vibrio Cholerae > Flashcards

Vibrio Cholerae Flashcards

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USMLE® Step 1 flashcards Biology 101 flashcards
1
Q

Biotypes of Vibrio cholerae O-1 ??

Difference in biological/biochemical reactions

A
  1. Classical
  2. E1-Tor

242

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2
Q

Serotypes of Vibrio cholerae (Classical )??

Antigen difference

A
  1. Inaba
  2. Ogawa
  3. Hikojima

242 IOH

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3
Q

Serotypes of Vibrio Cholerae (E1-Tor) ?

A
  1. Inaba
  2. Ogawa
  3. Hikojimu

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4
Q

Appearance of Vibrio cholerae on Gram Stain ??

A

Curved Comma shaped

242

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5
Q

Biochemical characteristics of V C ??

A

Oxidase (+) which differentiate them from enteric gram (-) bacteria

243

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6
Q

Which colour is V C

A

Blue
Oxidase (+)

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6
Q

Virulence factor of VC ??

A
  1. Enterotoxin
  2. Pili
  3. Mucinase

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7
Q

Toxin name of V C ??

A

Enterotoxin - Choleragen

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7
Q

VC vs Enterobacteriaceae ??

A
  1. Single polar flagella
  2. Curved bacillus
  3. Oxidase (+)
  4. Aerobe

Opposite of enterobacteriaceae

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8
Q

Hnaging drop preparation appearance V C ??

A

Fish in stream in appearance

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9
Q

Antigen structure of V C ??

A
  1. O atg
  2. H atg

243

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10
Q

Media for V C ??

A
  1. Thiosulphate citrate bile sucrose TCBS agar
  2. Monsurs media - Tellurite Taurocolate Gelatin AgAR MEDIA
  3. MacConkey agar
  4. Nutrient agar

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11
Q

TCBS media appearance of VC ??

A
  1. Yellow colony
  2. Background- Dark-Green

243

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11
Q

Monsurs media appearance of VC ?

A
  1. Dark colony
  2. Characteristics hollows

243

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12
Q

BD stain of V C ??

A

V C O139 Bengal

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12
Q

High risk of V C infection ??

A
  1. Person have little/No Gastric acid - Take antacid
  2. Who had - Gastrectomy
  3. Blood group- O

244

13
Q

Subunit of Choleragen ??

A
  1. A = Active subunit
  2. B = Binding subunit

244

14
Q

A subunit function ??

A

ADP- Riborylation
Enters into the cell

244

15
Q

B subunit function of Cholerageen ??

A

Binds to a ganglioside receptor of enterocyte
GM1 = Activate Gs Pathway

244

16
Q

Why watery D in cholera ??

A
  1. C is not an invasive infection
  2. Choleragen toxin
  3. Inc secretion of Cl & H2O from the cells
  4. Watery D = No RBC or Neutrophills

244

17
Q

A subunit further action ??

A
  1. Stimulate adenyl cyclase
  2. Overproduction of cAMP
  3. pKa = Protein kinase = Activate transport channel = Cystric fibrosis

244

18
Q

Hallmark of cholera infection ??

A

Watery diarrhea

245

19
Q

MacConkey agar characteristics in V C ??

A

Colorless
Lactose fermented slowly

245

20
Q

Dark ground illumination DGI appearance of V C ??

A

Shooting star in dark sky

245

21
Q

Microscopic examination of V C ??

A
  1. Gram staining
  2. Hanging drop preparation
  3. DGI

245

22
Q

TSI agar characteristics??

A
  1. Acid slant
  2. Acid butt without gas / H2S
  3. Ferments sucrose

245

23
Q

Why monsur media dark black colony??

A

Liquifaction of gelation

245

24
Q

Biochemical characteristics of V C ??

A
  1. Urease (-)
  2. Oxidase (+)
  3. Reduce nitrate
  4. Produce indole
  5. Fermant sucrose & lactose
  6. No gas

Concept

24
Q

Tx of cholera ??

A

Azithromycin

246

25
Q

Vaccine of cholera ??

A

Vaxchora
Live attenuated vaccine
+ Tetracycline =Close contact

26
Q

Transport media for VC ??

A
  1. Alkaline peptone water
  2. Carry Blair Media

Concept

27
Q

Clinical features of V C ??

A
  1. Rice watery stool -
  2. Dehydration
  3. Cardiac F
  4. Renal F
  5. Acidosis
  6. Hypokalemia
  7. No Abdominal pain
  8. 40% case death

246

28
Q

Toxins act by inc intracellular cAMP ??

A
  1. Cholera toxin
  2. Labile T of E coli
  3. Pertusis T
  4. Edema factor of anthrax T

Concept

28
Q

Never casue extra intestinal infection ??

A
  1. V C
  2. Shigella

C

29
Q

Serological test for VC ??

A
  1. Slide agglutination test
  2. Rise in AB titre

Microbiology -Medical (29 decks)

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