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Microbiology -Medical-Prof > Antigen-Antibody/Immunological Reaction Test > Flashcards

Antigen-Antibody/Immunological Reaction Test Flashcards

1
Q

Definition of serological test

412

A

The tests used to detect Atg/AB in serum

412

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2
Q

Dangerous to culture ??

A

Rickettsial D

412

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2
Q

Use of serological test ??

A
  1. Dx of infectious D
  2. Dx of autoimmune D
  3. Determination of Blood type
  4. Determination of HLA type

412

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2
Q

Why Atg detection is superior to AB detection ?

A

Atg is more specific for Dx of D if Atg is detected it indicates the presence of target pathogen

412

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3
Q

Which organisms cannot culture ??

A
  1. Syphilis
  2. Hepatitis-A B C

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4
Q

Take to much time to grow ??

A

Mycoplasma

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5
Q

Dx of autoimmune D ??

A
  1. SLE=Ab against DNA
  2. Rheumatoid arthritis = Ab against IgG
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5
Q

Culture tecnique not readily available?

A

HIV
EBV

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6
Q

Name of Ag-Ab reaction ??

A
  1. Agglutination test
  2. Precipitation test
  3. Complement fixation test= CFT
  4. Immunoassay using labeled reagents

413

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6
Q

Name of agglutination test ??

ST LPH

A
  1. slide
  2. tube
  3. latex
  4. particle
  5. Hemagglutination

413

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7
Q

Complement fixation test use ?

A

Kala azar
Filarial parasite
Gonococcus

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8
Q

Immunoflurescence IF use ?

A

Detection of T B Cells
Detection of treponemal AB in syphilitic patients

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9
Q

Enzyme Linked Immuno Sorbent Assay ELISA use ?

A

HIV ab
Hepatitis B panel
HBsAg HBeAg anti hbs anti hbc

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10
Q

Direct agglutination test ??

A
  1. Slide
  2. Tube

414

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11
Q

Slide agglutination tets ??

A
  1. Blood grouping
  2. Serotype of bacteria

414

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11
Q

Tube agglutination test ??

A
  1. Widal test-Enteric fever
  2. Weil-Felix test-Rickettsial infection

414

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12
Q

Latex ??

A
  1. HBsAg test
  2. ASO titre estimation

414

12
Q

Indirect/Passive agglutination test ??

A
  1. Lat3ex
  2. Paticle
  3. Hemagglutination
  4. Co-agglutation

414

13
Q

Particle ??

A

Anti HIV ab

14
Q

Hemagglutination??

A

TPHS for Dx of syphillis

414

15
Q

Co-agglutination ??

A

atg from CSF in case of bacterial meningitis

414

16
Q

Indirect Hemagglutination test ??Procedure ??

A

RBC + Tannic acid/CrCl2 — (Tagged Atg)—-Pt serum —Serum contain AB – IF AB there will be hemagglutination
reverse can be occure
RBC= Sensitized RBC

415

16
Q

Use of coombs test ??

A
  1. Hemolytic disease
  2. Autoimmune hemolytic anemai

415

17
Q

Indirect HAG test ??

A
  1. Treponema pallidum H-TPHA -Syphillis
  2. Reverse passive H -RPHA - Hepatitis B

415

18
Q

Prozone phenomenon definition??

A

Inhibition of agglutination by excess AB

415

19
Q

M/A of PP ??

A

Each epitope of Atg is occupied by one AB & there is no clumping of Ag -
so no agglutination

415

21
Q

How to overcome PP ??

A

Dilutions of AB are added to identical conc of particulate Atg

415

22
Q

Agglutination definitoion ??

A

Atg= IS particulate or is an inert particle coated with an atg
AB=cross react with Atg=Latticework formed = Clumping

416

23
Q

Precipitation def ??

A

Atg in solution
AB cross react with Atg & aggregates (precipitates) form

416

23
Q

For the quantitation of either atg / ab in pt specimen which test ??

24
Q

Which ELISA detect Atg ??

A
  1. Sandwich
  2. Competitive

419

24
Q

ELISA procedure ??

A
  1. Bottom of the well=ATG
  2. Atg — Pt serum AB
  3. Pt serum AB — AB to human IgG
  4. AB to Human IgG — Enzyme
  5. Enzyme — Substarte
  6. Sub change the colour when it is acted upon by the E

419

25
Q

Types of ELISA ??

A
  1. Sandwich
  2. Competitive
  3. Direct
  4. In direct

419

26
Q

precipitation in solution example ??

A
  1. Detection of AB
  2. Detection of Atg

416

26
Q

Use of ELISA ??

A
  1. Hepatitis B
  2. HIV AB
  3. Toxoplasma AB

419

27
Q

Immunoflurescene procedure ??

Fluroscence AB test

A
  1. Known Atg is bind to the slide
  2. Pt serum
  3. If pt serum contain AB -it will stict to the atg –
  4. seen fluorescent dye-labeled AB to human IgG

419

28
Q

Precipitation in agar example ??

A
  1. measure IgG IgM
  2. measuree C3
  3. measur Creactive protein-CRP

416

29
Q

Precipitation in agar with an electric field??

A
  1. H influenza
  2. N meningitis
  3. S pneumoniae

416 Dx

30
Q

CFT in (+) reaction ??

A

Atg + AB (if present ) = complement fix
2nd stage
sensitized RBC —- no lysis –no hemolysis

30
Q

CFT (-) test ??

A

Atg + AB (NO) = complement fix NOT
2nd stage
sensitized RBC —- lysis – hemolysis

Microbiology -Medical-Prof (49 decks)

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