Enterobacteriaceae

Question 1

common characteristics of enterobacteriaceae ??

Answer 1

• facultative anaerobes
• oxidase -
• fermant glucose
• reduce nitrates to nitrites

219

Question 2

other features of E ?/

Answer 2

• G - rods
• motile with peritrichous flagella
• catalase +
• grow-bile containing MacConkey agar media

219

Question 3

only capsulated E ??

Answer 3

klebsiella

219

Question 4

Non motile E ??

Answer 4

SKY
Shigella
Klebsiella
Yersinia

219

Question 4

lactose fermented rapidly E ??

Answer 4

Klebsiella
E coli
Enterobacter

Question 5

lactose non-fermenter ?/

Answer 5

shigella
salmonella
proteus

Question 6

Lactose F slowly ?/

Answer 6

serratia
vibrio

Question 7

why Pseudomonas is not E >??

Answer 7

aerobe
doesnot ferment glucose
oxidase +
donot reduce nitrates

220

Question 8

Lactose + colour in Macconkey ??

Answer 8

pink

Question 9

colourless colony ?/

Answer 9

shigella
salmonelaa
proteus

Question 10

Pink colony by which E in macConkey media ??

Answer 10

e coli
\klebsiella
citobacter
E

Question 11

Diseases caused by Escherichia Coli ??

Answer 11

223

Question 12

source of E coli in UTI ?/

Answer 12

Colonic flors

Question 13

source of e coli in neonatal meningitis ??

Answer 13

mothers birth canal

Question 14

properties of E coli ?

Answer 14

222

Question 15

E coli VS Shigella /Salmonella ?/

Answer 15

SS = Donot ferment lactose

Question 16

UTI by E coli >???

Answer 16

223

Question 17

endotoxin mediated diarrhea causing baccteria ??

Answer 17

E coli
Vibrio cholerae

Question 18

Invasive inflammatory Diarrhea causing bacteria ??

Answer 18

shigella
salmonelaa\
compylobacteer jejuni
E coli O157:H7

Question 19

Classification of E coli ??

Answer 19

Enteropathogenic
Enterotoxigenic
Enteroinvasive
Enterohemorrhagic
Enteroaggregative

224

Question 20

why UTI common in women ?/

Answer 20

short urethra
proximity of urethra to the anus
colonization of the vagina by member of fecal flora

Question 21

enterohemorrhagic E coli diseases name ?

Answer 21

• Bloody Diarrhea
• Hemorrhagic colitis
• Hemolytic uremic syndrome

226

Question 22

why name shiga toxin ??

Answer 22

same toxin as shigella

Question 23

e coli Butt & slant colour ?

Answer 23

Yellow
Yellow

Question 24

M/A of diarrhea by E coli ?/

Answer 24

224

Question 25

MacConkey agar media sterilization ??

Answer 25

Tyndalization - 100

Question 26

anti-microbial susceptibility test name ?/

Answer 26

mueller hinton agar media
disk diffusion
dilution methos

Question 27

Muller hinton media type «

Answer 27

solid basic media

Question 28

M H media sensitive to what ??

Answer 28

Clear zone of inhibition

Question 29

Antibiotic disc in M H media ?/

Answer 29

Inhibit growth of bacteria

Question 30

enteropathogenic E coli cause ?

Answer 30

bloody D

Question 31

Travelers D cause by ??

Answer 31

Entertoxigenic E coli

Question 32

Tx of O157:H7 ??

Answer 32

ciprofloxacin

Question 33

E coli VS lactose fermenting other G - rods ?/

Answer 33

• produce indole from tryptophan
  decarboxylate lysine
  source of carbon - acetate
  motile

Question 34

Mode of transmission of EHEC ?

Answer 34

undercooked hambuerger
fast food restaurant
unpasturized juices
contact with animals - O157:H7

Question 35

M/A of shiga toxin ?

Answer 35

226

Question 36

how to prevent hemorrhagic collitis ?

Answer 36

cooking ground beef

28.2.2025
6.28PM

Question 37

UTI kara kore ?

Answer 37

E coli
enterobacter
proteus
serratia
klebsiella

Question 38

Enterobacteriaceae ki ki ache ??

Answer 38

E coli
shigella
salmonella
klebshiella
proteus
serratia

Question 39

Bacteroides aerobic or anaerobic ?

Answer 39

anaerobic
291

Question 40

why Exotoxins of E coli are called Heat labile and heat stable ? meaning of this ?

Answer 40

🔬 Heat-Labile (LT) vs. Heat-Stable (ST) Exotoxins in E. coli

• 💨 Heat-Labile (LT): Denatured @ >60°C
  
  • Activates Gsα → ↑cAMP
  • ⬆ Cl⁻ secretion, H₂O efflux → Watery diarrhea
  • Similar to Cholera toxin
  • Encoded by plasmid
• 🔥 Heat-Stable (ST): Resistant to heat (Stable @ 100°C)
  
  • Activates Guanylate cyclase → ↑cGMP
  • ⬇ Na⁺ reabsorption → Watery diarrhea
  • Types: STa (human) & STb (animal)
  • Encoded by plasmid or transposon

🦠 Diseases: Traveler’s diarrhea (ETEC)
💊 Tx: Supportive, rehydration, ± fluoroquinolones

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Question 41

MC media type ?

Answer 41

selective
indicator

Question 42

why MC indicator ?

Answer 42

neutral red

Question 43

E coli Virulence factors

Answer 43

LT
ST
Endotoxin
verotoxin
capsule
pili

Question 44

Ekjon Pt aslo - UTI suspect korcho ?? ekhon kivabe approach korba ?

Answer 44

Urine culture - whole

769

Question 45

which bacteria is suspected if colour of macconkey agar media in translucent ?

Answer 45

🦠 MacConkey Agar Interpretation

🔍 Translucent/Colorless Colonies = Non-lactose fermenter (NLF) → Possible Pathogen

🚨 Suspected Bacteria (Gram-negative rods):
1️⃣ Salmonella 🥚 (H2S + on TSI)
2️⃣ Shigella 💩 (H2S -)
3️⃣ Proteus (Swarming, Urease +)
4️⃣ Pseudomonas aeruginosa 💚 (Oxidase +, Fruity odor)

🧪 Confirmatory Tests: TSI, H2S, Urease, Oxidase, API

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Question 46

how urine sample collected ?

Answer 46

early morning mid-stream urine sample - 5ml - clean dry sterile screw cap container - sterelized test tube

urine sample should be properly labeled

to avoid contamination - urine should be collected after proper cleansing of genitalia