Mycobacterium Flashcards
Example of typical mycobacterium ??
T-BAL
- M Tuberculosis
- M Bovis
- M Africanum
- M Leprae
260
Atypical mycobacterium whose classification ???
Runyon classification
Runyun classification bacteria ??
- Photochromogens
- Scotochromogens
- Nonchromogens
- Rapid grower
260
Photochromogens who ??
Yellow orange pigment produce when exposed to light
260
Photochromogens example ???
- M Kansasii
- M marinum
m k 260
Scotochromogens who ??
Produce pigments only in the dark
260
Scotochoromogens example ??
M Scrofulaceum
260
Non chromogens example ??
- M Avium
- M intercellulare
260
Rapid grower mycobacterium ??
- M fortuitum
- M cholenei
260
M T Complex ??
- M Tuberculosis
- M Africanum
- M Bovis
- M Microti
261
Reason of acid fastness of Mycobacterium»_space;>
- High lipid content
- Integrity of cell wall
- Integrity of cell
261
What is acid fast ???
Organism ability to retain the carbolfuchsin stain despite subsequent tx with Ethanol-HCl mixture
Acid fast bacteria Example M ??
- M TUBERCULOSIS
- M BOVIS
- M LEPRAE
T BL
Features of Mycobacterium ??
- Nor G+ Nor G-
- Intracellular multiplication
- Aerobic
- Obligate aerobe
- No spore
261
Acid fastness example ???
NGL R
- Nocardia
- Rhodococcus
- Gordonia
- Legionella micdadei
Non features of mycobacterium ?
- Non Spore
- Non Motile
- Non Capsulated
262
Selective media of M Tuberculosis ??
- Lowenstein Jensen media
- Broth media
- BACTEC Broth media
- Dubos media
- Tissue culture media
becton-dickson-Mycobacterium growth inhibitory tube
262
Why lowenstein jensen media selective ??
Antibiotics & Malachite green
262
Virulence factors of Mycobacterium T ??
- Cord factor
- Sulphatides
- LAM - Lipo arabino mannan
- Heat shock protein
- MYcosides
- Superoxidase dismutase
- Mycolic acid
Cord factor microscopic view ??
Serpentine cords in which acid fast bacilli are arranged in parallal line
263
Function of cord factor ??
- Inhibit migration of leukocyte
- Chronic granuloma
- Serve as a immunologic adjuvent
263
Primary tuberculosis healed by ??
Fibrosis
263
Primary tuberculosis progression ??
- Progressive lung disease
- Bacteremia
- Miliary TB
- Hematogenous dissemination
263
Which type of hypersensitivity ?
Delayed
263
Pathogenesis of TB in one line ??
Anti Mycobacterial Cell mediated immunity
263
M Tuberculosis organ pathway ??
Inhalation - Lower lobes of lung - Macrophage
Ghon focus formation ??
Bacilli present in the macrophage recruit epithelioid & langhans cells
Ghon complex composition ??
G Focus + Regional lymph node-Lymphadenitis
263
Ghon focus which necrosis ??
Caseous necrosis
263
Giant cells formation»_space;??
CMI – APC – Present Macrophage – Th cell – IL-12 – Th1 — Granuloma + CN — IFN-Gamma — Macrophage activate – Epithelioid cell – Giant cells
263
Ghon focus features ??
Pale yellow caseous nodule
263
Ghon complex other name ??
Primary compleex of RANKE
263
10% outcome of Primary TB ??
- Miliary TB
- Intestinal TB
- T meningitis
- T plurisy
263
Progressive primary TB
90% Larent dormant TB fate ??
- 90% No Disease
- 10% Secondary TB
264
Secondary TB site ??
Apex of the upper lobes
264
Complication of secondary TB ??
Vertebral osteomyelitis Potts disease
264
Site of Primary TB ??
- Lungs
- Tonsil
- Skin
- Intestine
265
Main findings of primary TB ??
- Cough
- Hemoptosis
265
Extra pulmonary TB site ??
- Pleura
- Larynx
- Lymph nodes
- GIT
- Kidney
- Bone
- Joints
- Spine
- CNS
265
80% case of Pulmonary TB ?
80% infectious
- Fever
- Fatigue
- Sweat
- Weight loss
265
How M T cause damage without exotoxin or Endotoxin ??
Delayed hypersensitivity
Caseating granuloma + cavitation
tuberculin necrotizing toxin -TNT
Early secreted antigen-6
ESAT-6
271
265
Tubercle definiton»_space;??
Is a granuloma sourrounded by fibrous tissue — caseation necrosis
265
Tubercles heal by ??
- Fibrosis
- Calcification
265
Sputum (+) where ??
Secondary TB
266
Primary TB more dangerous why ??
- No prevoius immunity
- Miliary TB - Dissemination
- Meningitis
- Other organ involvement
267
Lesions produced by TB ??
- Exudative
- Granulomatous
Granulomatous lesion in TB ??
- Central - Giant cell + Tubercle bacilli
- Surround by - Zone of epithelioid cells
267
Culture of TB ?
- Lowenstein jenson
- Middle brook
- BDMGIT
Becton Dickinson M Growth Indicator Tube
267
Microscopy of TB ??
According to Sir lecture
267
- Light microscopy
- Fluroscence microscopy
267
Latent infection Dx ??
- PPD test
- IGRA
Rapid molecular diagnostic test ??
- True NAT
- Xpert
- MTB/XDR
- gene expert
- CBNAAT
267
Other test of TB ??
- PCR
- Line probe aasay LPA
- In terferone Gamma Release Assay=IGRA
- Luciferase assaay
- Tuberculin / Mantaux test
267
Tuberculin test /Mantoux test ??
Protein derivative - ID - Diameter of induration - (Thickening) - Erythema (Redding )
268
Modern investigation ?
- GeneXpert MTB/RIF test
- Molecular probe- DNA probe to detect RNA sequence
- HPLC
- Urine test to detect Lipoarabinomannan
269
PPD TEST procedures ??
Purified Protein Derivatives
0.1 ml 5TU ID
PPD indicators ??
- 15 mm - No known risk
- 10 mm - with high risk
- 5 mm - Deficient CMI
Close contact with a person having active TB
268
MDR TB drugs name ??
- Isoniazid
- Rifampicin
273
XDR TB drugs ??
- Isoniazid
- Rifampicin
- Fluroquinolone
- Kanamycin
- Amikacin
AFB stains interpretation ??
- > 10AFB = Report +++
- (1-10) AFB/field = R ++
- (10-100) AFB/field=R+
- (1-9) AFB/100 f = R the exact number
Biochemical test of TB ??
Produce Niacin
268
Role of GeneXpert in Dx of Pulmonary TB ??
- Identify M T complex
- Detects genes that encode Rifampicin resistance
269
False + in PPD ??
Skin test +
Patient has no TB
270
False - TB in PPD ??
Pt has TB
Skin test is -
270
Renal tuberculosis features ??
- Dysuria
- Hematuria
- Flank pain
- Sterile pyuria
Mycobactrium laprae diseae name ??
Leprosy /
Hansens disease
275
Infection time for Lepromatous leprosy ??
20 hours per week for atleast 3 months in a year
275
Why antibiotic therapy for long time ??
Grows very slowly
slowest growing human bacterial pathogens - 14 days
275
Lepromatous leprosy transmission ??
Prolonged duration + Close contact
275
Target cells for M leprae ??
Skin & Nerve cells (Schwann cells )
275
Nerve damage in leprosy pathogenesis ??
- Direct contact with the bacterium
- CMI attack on the nerves
275
Which nerve damage in Leprosy ??
Ulnar
275
2 Forms of leprosy «
- In tuberculoid leprosy
- In lepromatous leprosy
276
CMI to the organism is poor ??
Lepromatous leprosy
276
CMI strong ??
In tuberculoid leprsoy
276
Foamy histiocytes seen»_space;??
In L L
276
Granuloma containing giant cells are seen ??
IN tuberculoid leprosy
276
In LL multiple nodular skin lesions — face manifestation ??
Leonine /Lion like facies
Prominant brow
276
Which on is more contagious ??
LL»_space;»» TL
276
LL complications ??
- Leoline facies
- Necrosis of bones & cartilage
- Testicular atrophjy
276
Histopathology biopsy of LL ?
Foam cells - Lipid laden macrophages
Complications of TL ?
- Corneal ulcer
- Claw hand
- Foot drop
- Loss of digits
- Trophic ulcers
- Peripheral neuropathy
276
Histopathology biopsy result of TL ??
Typical granuloma
277
Lepra / Virchow cells ??
Lipid laden macrophage / Foam cells in nLL
279
LL serological test result / identification ??
IgM against Phenolic Glycolipid-1
but not in TL
276
Staining method in Leprae ??
Ziehl neelson staining with 5% H2SO4
279
Bacteria which cannot be cultureed in conventional culture media ??
- M Laprae
- Chlamydia
- Rickettsiae
- Treponema pallidum
CRTM
Lepra reaction ??
Acute exacerbation of any form of leprosy
279
Ziehl neelson staining result of M L ??
Bright red straight / curved rods arranged in parallel bundles/ Bundles of cigars
277
Features of lepra reaction»_space;
- Fever
- Raised ESR
- Erythema nodosum leprosum - Painful nodules neuritis uveitis
279
Tx of leprosy ??
- Dapsone
- Rifampicine
- Clofazimine
279
Lucio phenomenon ??
Multiple ulcer + Endarteritis in LL 279
Grown where ??
Mouse footpad / in the armadillo
reservoir for human infection
ZoonOTIC D
275
which part of body no TB ??
let alone
hair
nail
everywhere
265
stain name of TB ?
ziehl neelson
ziehl neelsen result ?
red bacilli
blue background
269
media culture time ?
6-8 weeks
tuberculin test hater kon side e ?
floxor aspect of the forearm
gene Xpert sylhet er kothay ??
rikabibazar
tuberculin test timimg ??
about - half an hour for immediate hypersensitivity
then advised to report after 72 hours
PCR of TB ki SOMC te hoy ???/
NO
WHy they are called atypical MYCOBACTERIUM ??
280
whole comparison
why late in culture media»_space;>
doubling time = 18 hours
slow grower
what is doubling time ??
262
BCG vaccne type ?
live attenuated vaccine
does BCG give lifelong immunity ??
NO
lIFE long protection na dileo keno deya hoy ??
children der TB prevent korar jonno
efficacy of BCG ??
70%
how many sputum sample is taken /
3
what is sterile pyuria ?
Sterile pyuria is the presence of elevated white blood cells (WBCs) in the urine (pyuria) without detectable bacteria in a routine urine culture
atypical vs typical ?
niacin -
280
Name the culture media only in renal TB with sterile pyuria ?
In cases of renal tuberculosis (TB) with sterile pyuria, the specific culture media used to isolate Mycobacterium tuberculosis are:
- Löwenstein-Jensen (LJ) Medium
- Middlebrook 7H10 Agar
- Middlebrook 7H11 Agar
- Middlebrook 7H9 Broth (used in liquid culture systems like BACTEC MGIT 960)
These media are specifically designed for the growth of mycobacteria, including M. tuberculosis, and are essential for diagnosing renal TB when sterile pyuria is present.
Method of pasteurization?
87
holding method
flash method
UHT
Mycobacterium bovis kivabe amader sorire ase ?
Unpasteurized milk
what is UHT ?
Ultra high temperature method
what is the meaning of ultra in UHT ?
133
FOR
2 sec
Principle of pasteurization?
87
speciality of TB ?
AFB
Some slowly , some rapid
intracellular multiplication
do not form spores
obligate aerobe
why M bovis cannot grow in LJ media ?
Glycerol sudhu M t er growth promote kore
SOMC te kon machine for TB dx ?
GeneXpert
LED microscope
Digital X-ray machine
why atypical M cannot grow in Lj media
malachite green inhibit kore
which atypical TB prevalence BD te ?
M bovis
bovine TB
Tuberculin / mantoux/PPD askin test name in Children ?
TUBERSOL
tuberculin purified protein
3.3.2025
2.36PM
pari nai
Generation time of M t & other Bacteria ?
Mt - 18 hr
E coli - 20 min
inoculation time of normal bacteria ?
18–24 hours at 35–37°C (under aerobic or anaerobic conditions, depending on the organism).
diseases caused by Atypical M ?
lunf disease
swiming pool granuloma / fish tank g
\scrofula
skin infections
why red-blue color in Ziehl neelson stain ?
Red color: Due to retention of carbol fuchsin by the acid-fast mycolic acids in the mycobacterial cell wall.
Blue background: Due to the counterstain (methylene blue or malachite green) applied after decolorization.
why this stain name so ? give me answer from levinson in 2 line
The Ziehl-Neelsen stain is named after two microbiologists: Franz Ziehl, a German bacteriologist, and Friedrich Neelsen, a German pathologist. They modified the staining technique to identify acid-fast bacteria like Mycobacterium tuberculosis. (Source: Levinson’s Review of Medical Microbiology and Immunology).
process of staining in short
The Ziehl-Neelsen (ZN) staining process for acid-fast bacteria like Mycobacterium tuberculosis involves the following steps:
- Primary Staining: Flood the slide with carbol fuchsin and heat gently to allow the dye to penetrate the mycobacterial cell wall.
- Decolorization: Rinse with n20% - H2SO4 acid-alcohol to remove the dye from non-acid-fast cells.
- Counterstaining: Apply methylene blue to stain non-acid-fast cells and the background.
Result: Acid-fast bacteria (e.g., M. tuberculosis) appear red, while non-acid-fast cells and the background appear blue.
Acid fast stain name <
Z-N
kinyoun
fluroscent - auramine rhodamine
which side of human is effected by M bovis <
ileocecal region
2nd TB site ?
jekhane O2 beshi
apex of the upper lobe
264
culture time ? MT ?
premanondo sir .
4-6 week
tuberculin test hypersensitivity type ?
4
4-6 week e culture check korba na ?
check korte hobe
Why mycobacterium leprae cannot grow in culture ?
Mycobacterium leprae, the causative agent of leprosy, cannot grow in standard laboratory culture media due to the following reasons:
-
Obligate Intracellular Pathogen:
- M. leprae requires a highly specialized environment within host cells (e.g., Schwann cells and macrophages) to survive and replicate. It cannot metabolize or grow independently outside a host.
-
Extremely Slow Growth Rate:
- M. leprae has an exceptionally slow replication time (approximately 12–14 days), making it difficult to cultivate in vitro.
-
Complex Nutritional Requirements:
- The bacterium relies on host-derived nutrients and factors that cannot be replicated in artificial culture media.
-
Genome Reduction:
- M. leprae has a highly reduced genome with many non-functional genes, limiting its ability to synthesize essential metabolites required for growth in culture.
Alternative Methods for Study:
- Animal Models: M. leprae can be grown in the footpads of mice or armadillos, which provide a suitable host environment.
- Molecular Techniques: PCR and genomic studies are used to detect and study M. leprae without the need for culture.
(Source: Levinson’s Review of Medical Microbiology and Immunology)
kothay kivabe M leprae culture korbe ?
Alternative Methods for Study:
- Animal Models: M. leprae can be grown in the footpads of mice or armadillos, which provide a suitable host environment.
- Molecular Techniques: PCR and genomic studies are used to detect and study M. leprae without the need for culture.
karo TB hocche , karo hocche na emon keno ?
malnutrition
some people effected bt TB whether some are not ? why so ? give me answer in 3 line from levinson ?
The variation in susceptibility to tuberculosis (TB) is due to differences in host immunity and genetic factors. Some individuals have strong cell-mediated immunity that controls Mycobacterium tuberculosis infection, while others with weakened immunity (e.g., due to HIV, malnutrition, or diabetes) are more likely to develop active disease. (Source: Levinson’s Review of Medical Microbiology and Immunology).
intracellular organism kivabe kille kore ?
CMI
Lab Dx of leprae ?
modified Z-N staining
how modified Z-N stain prepare /
5% H2SO4 = M l
//
//
20% H2SO4 = Mt
51 page
prem sir ques
lperosy hospital in sylhet ,
kazirbazar - sheikhghat
leprosy bangla ?
kustho
why skin & ulnar nerve >
grows in = optimal T for growth = 30*
which is less than body temperature
grows preferentially
275
site of leprosy attack >
skin
superficial nerve - ulnar nerve
leprosy kader hoy ?
Leprosy (Hansen’s disease) is caused by infection with the bacterium Mycobacterium leprae. It primarily spreads through prolonged close contact (e.g., respiratory droplets from the nose and mouth of an infected person). However, not everyone gets infected, as host immune system and genetic factors play a significant role. (Source: Levinson’s Review of Medical Microbiology and Immunology).
leprosy other name <
hansens disease
lepromin test - why ?
immune system weak hoye jay tai
lepromin test interpretation?
The lepromin test interpretation is as follows:
-
Positive Reaction (Induration >5 mm):
- Indicates a cell-mediated immune response to Mycobacterium leprae, seen in tuberculoid leprosy or healthy individuals exposed to the bacteria.
-
Negative Reaction (No Induration):
- Suggests anergy (lack of immune response), commonly seen in lepromatous leprosy or immunocompromised individuals.
(Source: Levinson’s Review of Medical Microbiology and Immunology).
culture media of Mt kothay rakhba >
incubator
kivabe M leparae spread kore ?
prolonged duration
close contact
20hours per week for atleast 3 months in a year
generation time of M l ?
14 days
275
BCG full form /
bacilli calmette guerin
immunity % of BCG <
developed - 80%
underdevelopeed = 0%
why TB beshi hocche ?
developing country
low socio-economic status
humidity beshi air er
overpopulated
site and time and dose of BCG ?
0.05ML
skin of the lower part of the deltoid region - left arm
after birth
ID
Lj slop er sathe rerlated >
Pt er kun position theke smaple nicchi
Mt keno macrophage kill korte pare na ?
intracellular surviva
low grade fever can be occur in which disease - 5 name
Low-grade fever can occur in a variety of diseases. Here are five examples:
- Tuberculosis (TB)
- Brucellosis
- HIV/AIDS
- Lymphoma
- Chronic Urinary Tract Infections (UTIs)
These conditions often present with persistent or recurrent low-grade fever as a symptom. (Source: Clinical Medicine and Pathophysiology).
MT er sathe mycology er kono relation ache ?
At first TB was thought to be a fungal disease
tai Myco-name
vaccinated pt e tuberculin test ?
+
> 10 mm