Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Unit 5 Lab Exam > Flashcards

Unit 5 Lab Exam Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

The pour plate technique is a common laboratory method used when counting ________ microorganisms

A

living

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What type of plate uses the beads to mix

A

spread plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

The beads used in the spread technique are ________; pour about ___-_______ beads

A

sterile; 5-10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

how is dilution factor calculated

A

1/ dilution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the dilution and dilution factor of the following: you take 1 mL of your sample and mix it with 99 mL of diluent

A

dilution total: 100mL
dilution factor: 1/100mL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is a straight forward way to count the number of living bacteria in a sample

A

spread a small sample on a petri dish and count the number of colonies through serial dilutions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the statistically significant countable number of colonies on a plate

A

30-300 colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is serial dilutions

A

a way of making very large dilutions accurately using small volumes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

In microliters what is the range of measurement for the following pipette:

P20 (yellow dot)

A

2 ul - 20ul

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

In microliters what is the range of measurement for the following pipette:

P200 (yellow dot)

A

20 ul - 200 ul

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

In microliters what is the range of measurement for the following pipette:

P1000 (blue dot)

A

200ul - 1000 ul

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Explain the two stop system

A

Hit resistance to suck up the intended amount and when you push it out do it fully past the resistance

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Where do you want to submerge the tip of pipettes

A

just below the meniscus of the liquid to be transferred and slowly aspirate the solution

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

DO NOT GET ANY LIQUID IN THE _____ OF THE MICROPIPETTE

A

BORE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What two ways can you get liquid into the bore of the pipette

A
  1. tipping it up
  2. having bubbles of the solution that can shoot up into the bore
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Spectrophotometer is a machine that has a light source and a detector to measure _________

A

transmittance

17
Q

When one is measuring a cell sample in the spectrophotometer, one is measuring

A

the light scattering or transmission

18
Q

Turbidity is a measurement with the _____________-

A

spectrophotometer

19
Q

What are the two ways we will do viable cell count by plating

A

spread plates with glass beads

pour plates with melted medium

20
Q

(T) aka % transmittance =

A

percent of light that reaches the detector

21
Q

What is optical density (1/T)

A

measure of amount of light scattering

22
Q

_______ OD = more light scattering = ______ cells

A

higher; more

23
Q

What is viable count measuring

A

cell’s ability to form a colony

24
Q

To accurately take an absorbance reading, a _______ wavelength of light must be used since samples may scatter or absorb different wavelengths of light differently

A

single

25
Q

What is the single wavelength selected by

A

monochromator

26
Q

What do monochromators do

A

they break white light into its component wavelengths

27
Q

What is our way of direct counting

A

petroff-hausser or hemacytometer

28
Q

What is an advantage of viable cell count

A

it helps us observe the dynamic changes in bacterial growth–live cells

29
Q

Does direct cell counting count both live and dead cells

A

yes

30
Q

There are _________ cells in a liquid sample than a solid sample

A

fewer

31
Q

What is the log phase

A

actively growing phase; this is the period when the microbes are growing and dividing at a steady rate

32
Q

When is the lag phase

A

right away as the microbes adjust to their environment

33
Q

What is the stationary phase

A

growth of microbes slows down because nutrients have been exhausted

34
Q

bacteria replicate via

A

binary fission

35
Q

How do we plot the rate of growth:
X axis
Y axis

A

time unit

cell number

36
Q

What is growth rate

A

the number of generations (doubling) per hour

37
Q

What is generation time

A

time it takes for the population to double

Microbiology (29 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions