Lab Exam Chapter7 Flashcards

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1
Q

What are the most gene sequence that is used for genetic identification

A

small subunit ribosomal rna

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2
Q

What does identification of SSU rRNA involve

A

sequencing the gene from your microbe of interest and matching the sequence to known sequences in a database containing the SSU rRNA genetic sequence from thousands

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3
Q

What are antibodies

A

proteins produced by the immune system of mammals that specifically bind to certain molecules called antigens

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4
Q

what are antibodies capable of

A

recognizing and binding to a molecule called an antigen with very high specificity

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5
Q

The antigen binding portion of the antibody is highly ______ between antibodies and each antibody recognizes a different antigen

A

variable

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6
Q

What is the result in a n antibody solution containing multiple different antibodies refferes to as

A

polyclonal antibody preperation

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7
Q

What do most antigens do

A

induce an immune response and are thus called immunogens

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8
Q

________ are the best antigens

A

proteins

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9
Q

What is the recognition sit of an antibody on an antigen

A

epitope

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10
Q

A _______ protein can have _______ epitopes and be recognized by several antibodies

A

single; multiple

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11
Q

T/F antigens may be soluble

A

true

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12
Q

example of soluble antigen

A

secreted protein toxins

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13
Q

example of antigens that are particulate

A

entire microbial cells

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14
Q

each pathogenic microbe produces dozens of different ________ and those are mostly secreted proteins, or cell surface attached proteins

A

antigens

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15
Q

When there is antigen excess or antibody excess the complexes formed will be ______

A

soluble

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16
Q

When there is antigen and antibody that is equal it will be _______

A

insoluble

17
Q

What are insoluble antigen and antibody reactions called

A

immunoprecipitation

18
Q

When can insoluble complexes of antigen-antibodies be visualized

A

by trapping the reagents and products in agarose (a purified form of agar that acts like a mesh)

19
Q

how do you get the antibody solution and an antigen sample into adjacent wells in an agarose-containing dish

A

pipetting

20
Q

What is a positive reaction for serological method of microbial identification

A

if the antigen and antibody enter the same location of the dish, and if the antibody recognizes the antigen, they will form a visible precipitate in the agarose

21
Q

what does direct agglutination results show when soluble antibodies come in contact

A

clumping of a particulate antigen

22
Q

What does passive agglutination result when the antigen or antibody has been couple to

A

insoluble particles such as latex beads

23
Q

Antibodies can be chemically modified with ______ and used to detect antigen on intact cells by detection of its activity

A

enztme

24
Q

What are these enzymes called

A

enzyme immunoassays