Lab microbes and mediums to know Flashcards
What bacteria type is E. coli
gram negative cell wall
What shape is E. coli
bacillus shaped
Does E. coli fermentate
yes it fermentated glucose and lactose
Is E. coli motile?
yes
What bacteria type is P. fluorescens
gram negative cell wall
What shape is P. fluorescens
bacillus shaped
Does P. fluorescens fermentate
no
Does P. fluorescens have the ability to be motile
yes flagellum for mobility
Where was their growth in the test tube for P. fluorescens, what type of oxygen relationship
Growth at the top; obligate aerobe
What type of oxygen relationship is E. Coli
facultative anaerobic bacterium
What type of bacteria is B. cereus
Gram positive
What shape is B. cereus
bacillus shaped
Is B. cereus motile
yes
What type of oxygen relationship does B. cereus have
aerobic or facultatively anaerobic
Does B. cereus fermented lactose and glucose
yes
Is S. Cerevisiae a bacteria
no–chitin cell wall
What type of shape is S. Cerevisiae
ovoid-shape
What type of growth was seen in the thioglycolate of E. faecalis; what’s its oxygen relationship
growth throughout; facultative anaerobe
What was seen in the thioglycolate of S. marcescens; what’s its oxygen relationship
Cloudy throughout with dark red at the top; facultatively anaerobe
What type of bacteria is S. epidermidis
gram positive
What shape is S. epidermidis
coccus
Is S. epidermidis + for glucose fermentation
yes
Is S. epidermidis motile
yes
What type of medium is nutrient broth/NA
all purpose medium
What type of medium is MAC
selective and differential medium (for gram negative bacteria)
What will MAC look like if fermentation occurs after 24 hours of incubation
red colonies
What type of medium is APT w/ tween
non selective (typically fastidious bacteria)
What are the differential ingredients in MAC
lactose
neutral red
What are the selective ingredients in MAC
bile salts
Crystal violet
What is the selective ingredient in APT w/ tween
sodium azide
What can thioglycolate help determine
obligate anaerobes
What type of medium is thioglycolate
differentail
What type of medium is fermenting broth
differential medium for fermentation
What color will carbone fermentation broth turn if fermentation has occured
yellow due to the bromcresol purple
What type of medium is EMB
selective and differential
What is the selective ingredient for EMB
Esoin Y and Methylene blue dyes
What is the differential ingredient for EMB
lactose
