Lab microbes and mediums to know Flashcards

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1
Q

What bacteria type is E. coli

A

gram negative cell wall

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2
Q

What shape is E. coli

A

bacillus shaped

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3
Q

Does E. coli fermentate

A

yes it fermentated glucose and lactose

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4
Q

Is E. coli motile?

A

yes

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5
Q

What bacteria type is P. fluorescens

A

gram negative cell wall

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6
Q

What shape is P. fluorescens

A

bacillus shaped

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7
Q

Does P. fluorescens fermentate

A

no

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8
Q

Does P. fluorescens have the ability to be motile

A

yes flagellum for mobility

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9
Q

Where was their growth in the test tube for P. fluorescens, what type of oxygen relationship

A

Growth at the top; obligate aerobe

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10
Q

What type of oxygen relationship is E. Coli

A

facultative anaerobic bacterium

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11
Q

What type of bacteria is B. cereus

A

Gram positive

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12
Q

What shape is B. cereus

A

bacillus shaped

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13
Q

Is B. cereus motile

A

yes

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14
Q

What type of oxygen relationship does B. cereus have

A

aerobic or facultatively anaerobic

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15
Q

Does B. cereus fermented lactose and glucose

A

yes

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16
Q

Is S. Cerevisiae a bacteria

A

no–chitin cell wall

17
Q

What type of shape is S. Cerevisiae

A

ovoid-shape

18
Q

What type of growth was seen in the thioglycolate of E. faecalis; what’s its oxygen relationship

A

growth throughout; facultative anaerobe

19
Q

What was seen in the thioglycolate of S. marcescens; what’s its oxygen relationship

A

Cloudy throughout with dark red at the top; facultatively anaerobe

20
Q

What type of bacteria is S. epidermidis

A

gram positive

21
Q

What shape is S. epidermidis

A

coccus

22
Q

Is S. epidermidis + for glucose fermentation

A

yes

23
Q

Is S. epidermidis motile

A

yes

24
Q

What type of medium is nutrient broth/NA

A

all purpose medium

25
Q

What type of medium is MAC

A

selective and differential medium (for gram negative bacteria)

26
Q

What will MAC look like if fermentation occurs after 24 hours of incubation

A

red colonies

27
Q

What type of medium is APT w/ tween

A

non selective (typically fastidious bacteria)

28
Q

What are the differential ingredients in MAC

A

lactose
neutral red

29
Q

What are the selective ingredients in MAC

A

bile salts
Crystal violet

30
Q

What is the selective ingredient in APT w/ tween

A

sodium azide

31
Q

What can thioglycolate help determine

A

obligate anaerobes

32
Q

What type of medium is thioglycolate

A

differentail

33
Q

What type of medium is fermenting broth

A

differential medium for fermentation

34
Q

What color will carbone fermentation broth turn if fermentation has occured

A

yellow due to the bromcresol purple

35
Q

What type of medium is EMB

A

selective and differential

36
Q

What is the selective ingredient for EMB

A

Esoin Y and Methylene blue dyes

37
Q

What is the differential ingredient for EMB

A

lactose