Unit 1 Lab Exam

Question 1

What must be placed on your lab materials

Answer 1

First and last name
Pod number
Date
Description of the microbe in the container

Question 2

Where should plates be labeled

Answer 2

the bottom half of the plate (the back of the half that contains agar)–never on the removable lid

Question 3

How do you place your plates to incubate

Answer 3

with the medium side up AKA inverted position

Question 4

What happens if you don’t incubate the plates with the agar side down?

Answer 4

moisture collecting on the top of the lid can drop onto the developing colonies, causing them to run together

Question 5

Define BSL-1

Answer 5

basic level of protection for agents that are not known to cause disease in normal, healthy humans

Question 6

Define BSL-2

Answer 6

appropriate for handling moderate-risk agent that cause human disease of varying severity by ingestion or through percutaneous or mucous membrane exposure

Question 7

Define BSL-3

Answer 7

appropriate for agents with a known potential for aerosol transmission, for agents that may cause serious and potentially lethal infections and that are indigenous or exotic in origin

Question 8

Define BSL-4

Answer 8

exotic agents that pose a high individual risk of life-threatening disease by infectious aerosols and for which no treatment is available are restricted to high containment labs

Question 9

Define fomite

Answer 9

an inanimate object capable of transmitting microbes

Question 10

What is considered a biologically contaminated material

Answer 10

is anything that comes in contact with a living culture (slides, tubes, flasks, petri disk)

Question 11

Where should tubes containing cultures be placed

Answer 11

in the slanted metal trays on the disposable cart

Question 12

Where should petri plates be placed

Answer 12

into the small orange garbage cans near the disposable cart

Question 13

Where should pipette tips and microfuge tubes be placed

Answer 13

discarded into the aluminum pipette tray on the pod