Lab exam chapter 10

Question 1

microbiocidal agents harmless to be applied to the skin and mucous membranes but they should not be taken internall

Answer 1

antiseptic

Question 2

agents that kill microorganisms but no spores

Answer 2

disinfectants

Question 3

chemical agents produced by microbes that can ill or inhibit certain microbs

Answer 3

antibiotic

Question 4

What must antibiotics pass through in gram negative microbes

Answer 4

porins

Question 5

What must anitbiotics pass through in gram positive organisms

Answer 5

thick peptidoglycan layer

Question 6

What do antibiotics target

Answer 6

bacteria specific components of essential cellular functions

Question 7

Why won’t antibiotics affect human metabolism

Answer 7

humans don’t have the enztmes or structures that the antibiotic inhibits

Question 8

What does the phenol coefficient test measure

Answer 8

the effectivenenss of chemical biocides (antiseptics and disinfectants)

Question 9

What does the Kirby bauer test used for

Answer 9

used for antibiotics

Question 10

How do you calculate the PC

Answer 10

the highest dilution that kills all organisms after incubation for 10 minutes but not after 5 minutes AND comparing this to phenol

Question 11

Biocides that had PC greater than 1 are

Answer 11

more effective than phenol

Question 12

Biocides that have PC less than 1 are

Answer 12

less effective than phenol

Question 13

Whta is the PC equation

Answer 13

(highest biocide inhib)/highest phenol inhibit

Question 14

What does ampicillin affect

Answer 14

peptidoglycan synthesis

Question 15

What does bacitracin affect

Answer 15

peptidoglycan synthesis

Question 16

What does ciprofloxacin affect

Answer 16

DNA replication

Question 17

What does kanamycin affect

Answer 17

protein synthesis

Question 18

If there is increased zone of clearing then it mean

Answer 18

increased sensitivity