Protein Synthesis Flashcards
Making a protein using the code of the nucleotides in DNA
Protein Synthesis
What are the 2 steps in protein synthesis ?
1) Transcription
2) Translation
Process copying a portion of DNA (gene) producing a complementary strand of RNA
Transcription
Describe protein synthesis in Prokaryotes
Replication, transcription, and translation occurs in the cytoplasm at the same time
Describe protein synthesis in Eukaryotes
Replication, transcription, and translation are separated by time and location.
Transcription: nucleus
Transcription: cytoplasm
How many different possible outcomes of 3-base codons ?
64
61 out of 64 of the 3-bsse codons are used for
Used for code for amino acids
What are the 3-base codons not if the 61 used for ?
3 of them are “stop codons “ the signal the end of the protein
What is the remaining 1 codon used for that is not part of the 61 or 3 to end?
That one codon that can specify methionine or serve as the “start” codon for protein synthesis
What is the start codon ?
Methionine
Is this Transcription or Translation?
1) RNA polymerase (enzyme) attached to the part of the DNA strand, separating a section of the DNA strand. (Not the entire strand )
2) RNA polymerase uses one DNA strand as template to assemble nucleotides into a complementary strand of mRNA
3) mRNA separates from the DNA strand and leaves the nucleus. DNA strands rejoin/zip closed
Transcription
Is this Transcription or Translation?
1) mRNA leaves the nucleus and attached to a ribosome
2) the tRNA molecule with the correct anticodon attaches to the mRNA codon
3) the amino acid is released from the tRNA. Peptide bonds is formed between amino acid
4) the mRNA slides through ribosome. The next tRNA attached to the mRNA codon.
5) this process continues until a STOP codon is reached
Translation
The genetic code is the _____ for every living organism, which means the codons represent the same amino acids in ALL living organisms
Same
Transcribed in nucleus - not all bases wil be translated into a protein
Pre-mRNA
DNA bases that are transcribed into mRNA and eventually code for amino acids in proteins ( expressed genes )
Exons