Microscopy Flashcards
What is a microscope
instruments which produce a magnified image of an object a simple pair of convex glass lens can magnify an object
what does the long wavelength in light microscopes mean
can only distinguish objects as seperate entities which are more than 0.2 micrometers away
what is an object
the material placed under the microscope
how to work out magnification
magnified image over actual image size
what is resolving power
minimum distance that a microscope can observe two objects as separate items the resolving power depends on the wavelength
what is cell fractionation
cells are broken up and organelles are released and seperated out
what conditions are the tissue placed in
cold to prevent denaturation of enzymes due to high temperature, buffered ph solution to ensure ph is constant as may effect enzyme activity and also sae water potential to prevent cell from bursting as a result of osmotic gain
what is a homogeniser
the cells are homeogenized which is like a blender it releases organneles from the cell the resultant fluid is called the homogenate as the whole cells are removed and large pieces of debris
the what is ultracentrifugation
the homogente is spun at high speed creating a centrifugal force which forces heaviest sediment at the bottom forming a pellet the remaining supernatant are removed and spun at an even faster speed
