Hive Flashcards
What is the structure of HIV
the outside is a lipid envelope with attachment protiens inside is a protien layer called the capsid that encloses the RNA and reverse transcriptase
how does HIV replicate
the HIV enter the bloodstream and ciculates the body it binds to the protien CD4 which is usually found on helper t cells this mean HIV binds to helper t cells then the protien fuses with the cell membrane and the rna and transcriptase enter the helper t cell the reverse transcriptase forms HIV RNA INTO DNA this is then inserted into the helper t cells DNA this is then makes MRNA - HIV using the cells enzymes the MRNA then travels out of the nucleas and attaches to the ribosomes and formulate hiv particles the hiv then leaves the cell attaching some of the celll surface membrane which form lipid envelope
how to carry out the elisa test
apply the sample to the surface which all the antigens in the sample will atch and wash the surface several times to remove excess unattached antigens, add the antibody that is pecific to the antigen and wash again several times add a second antibody which binds to the first antibody this time with an enzyme attached to it
hen add the colorless substrate the substrate will change color when an enzyme substrate complex is formed
the amount of antigen preesnet is proportional to the strngth of the color
how does antibiotics works
it prevmt cell wall cross linkages forming this weakens the walls forming in bacterial cell so water can enter the cell and bust virus dont have any metablolic pathway so nothing can be destriyed in order to destroy a virus
