Microbio - all! Flashcards
Osteoperiostitis of the alveoli of the teeth
Secondary to gingivitis
Magitot’s disease
Blue pus
By pseudomonas aeruginosa
Charrin’s disease
Durand’s disease is caused by
Chlamydia trachomatis
Erythrasma is caused by
Corynebacterium minutissimum
Tuberculous pericarditis
Cirrhosis of the liver
Hutinel’s disease
Hutinel’s disease is caused by
Mycobacterium tuberculosis
Lymphocytic choriomeningitis
Armstrong’s disease
Tyzzer’s disease is caused by
Bacillus pilliformis
Chromoblastomycosis
Cauliflower like lesion
Chromomycosis
Inflammation of tunica intima of cerebral arteries
Heubner’s disease
Self limited diarrhea
Un pasteurized milk and untreated well water
Brainered diarrhea
Phycomycosis, mucormycosis
Zygomycosis
Epidermodysplasia verruciformis
Red-violet lesions
Tendency to become malignant
Lewandowsky lutz disease
Maduramycosis
Madura foot
Mycetoma
Infectious ecematoid dermatitis
Engman’s disease
Fourth disease
Duke’s disease
Echinococcus multilocularis larvae infection
Hydatid disease, alveolar
Sepsis of cirrhotic patient
Oyster ingestion
Vibrio vulnificus
Chromomycosis is caused by …
Phialophora verrucosa, fonsecaea pedrosoi and
Clasposporium carrioni
Prion disease
Mad cow disease
Degenerative brain disorder
Bovine spongiform encephalopathy
Rapidly progressive septicemia
Puncture wound from Fish spine
Fish-slime disease
Diamond skin disease is caused by…
Strep pyogenes
Lethargic encephalitis
Economo’s disease
Endartritis obliterans
Friedlander’s disease
Vesicles on penis or vagina
Durand’s disease
Brown-symmers disease is an acute encephalitis. What are the viral pathogens that causes this?
Rabies Rubella Mumps Measles Influenza
Echinococcus granulosus infection
Hydatid disease, unilocular
Acute miliary TB of larynx and pharynx
Isambert’s disease
Duke’s disease is caused by…
Coxsackie and echovirus
Erysipelothrix insidiosa
Meat and fish handlers
Erysepeloid
Osteochondritic separation of epiphyses due to syphilis
Wegner’s disease
Transmission of armstrong’s disease
Food or dust contaminated by rodents
Rheumatic endocarditis
Delayed sequel for pharyngeal infection by group B strep
Boulilauds disease
Engman’s disease is caused by
Staph
Mycetoma is caused by
Allescheria boydii or actinomycetales
Polymorphous skin eruption
Etiology unknown
Habermann’s disease
Congenital syphilis
By treponema pallidum
Duboi’s disease
Superficial bacterial skin infection
Erythrasma
Syphylitic disease of the bursa
By treponema pallidum
Verneuil’s disease
Liver and intestine necrosis
Contact with rodents or dogs
Tyzzer’s disease
Armstrongs’s disease is caused by
Lymphocytic choriomeningitis virus LCM of arena virus
Swine erysipelas
Acute febrile vascular disease
Diamond-skin disease
Economo’s disease is caused by
Neurotropic virus
Diseases caused by coxsackie B3 and B5
Devil’s eye
Myocarditis and pericarditis
Pleurodynia (Bornholm’s disease)
Rimantadine and amantadine action
Prevents viral un coating in influenza A
CMV infection recurrence in immunocompromised patients, especially AIDS patient.
CMV retinitis
Re activation to zoster from…
Dorsal root ganglia
Incubation period of chickenpox
10-21 days
Glycoprotein the breaks down neuraminic acid, an important component of mucin
NA
Prion disease
Kuru
Gerstmann-straussler disease
Prion (13-3-3 protein)
Positive sense RNA virus
PCCRaFT Picornavirus Calicivirus Coronavirus Reovirus Flavivirus Togavirus
Most common viral encephalitis in the Philippines
Japanese encephalitis
Negative sense RNA viruses
"Always Bring Polymerase Or Fail Replication" Arenavirus Bunyavirus Paramyxovirus Orthomyxovirus Filovirus Rhabdovirus
Diagnosis for parvovirus
PCR
Primary infection of HSV 1
Gingivostomatitis - painful group of vesicles on the lips and mouth, which ulcerate and has usually without leaving a scar. Often accompanied by fever and viral symptoms
Family of hepa D virus
Deltavirus
Type of RNA virus which are like mRNA, they are immediately translated by the host’s ribosome into protein
Positive sense RNA virus
Diagnostic preparation in isolating HSV
Tzanck smear, cowdry A
Coxsackie A or B:
In mice, it causes paralysis and death with multiple organ damage
Coxsackie B
Diseases caused by poliovirus
Mild flu-like illness
Nonparalytic poliomyelitis: Aseptic meningitis
Paralytic poliomyelitis
Hepatitis that is chronic, cirrhosis, carcinoma and carrier
Hepatitis C
Protein found in paramyxovirus that results in multinucleated giant cells (called syncytial cells)
F protein
Non defective parvovirus strain
B19 virus
Reoviruses
Respiratory, Enteric, Orphan
Transfusion hepatitis
Hepatitis C
Kluver-bucy syndrome
Herpes encephalitis
Polio virus initially replicated in the tonsils and Peyer’s patches, spreading to the blood, and across cns barrier to the (anterior,posterior) horn of the spinal cord.
Anterior horn cells
Intact virus of hepatitis B
Dane particle
Hepatitis that may cause hepatocellular carcinoma
Hepatitis B and C
All RNA virus are SIngle stranded, except…
Rotavirus
Reovirus
Disease caused by parvovirus which ensues when the virus stops the production of RBCs in the bone marrow
Transient aplastic anemia crisis
Transmission of poliovirus
Fecal-oral transmission
Rhinovirus is acid-labile or acid-stable?
Acid-labile
Also known as break bone fever
Dengue fever
Treatment for CMV infection
Ganciclovir
Caliciviruses
Norwalk virus
Hepatitis E
Type of RNA virus that is transcribed in reverse fashion into DNA
Retrovirus
Most common cause of common cold
Rhinovirus
Papovavirus
Polyomavirus that causes mild and ASYMPTOMATIC infection in children. Also seen from immunosuppressive kidney transplant patients and HPV.
BK polyomavirus
RNA virus has this enzyme for synthesis.
RNA-dependent RNA polymerase
Building block of capsid
Capsomer
Herpesvirus that cause infectious mononucleosis
EBV
RNA viruses that cause gastroenteritis
Rotavirus (reovirus)
Calicivirus
Causes adult acute t-cell leukemia and tropical spastic paraparesis (partial paralysis of lower limb)
Human T lymphotrophic virus type 1 (HTLV-1)
Retrovirus has this required enzyme for synthesis
Reverse transcriptase
Virus that cause common cold and has prominent halo and spikes
Coronavirus
TORCHES can cross blood-placental barrier
Toxoplasmosis Rubella CMV Herpes, HIV Syphilis
Most frequent cause of viral (aseptic) meningitis
Mumps virus
Coxsackie
Echovirus
Intranuclear inclusion bodies seen in yellow fever virus infection
Councilman bodies - acidophilic inclusion
Most important antigen of EBV
Viral capsid antigen (VCA)
Diseases caused by HSV 1
Gingivostomatitis
Encephalitis
Keratoconjunctivitis
Herpes labialis
Largest virus
Poxvirus
Poxvirus that is Completely eradicated worldwide
Variola virus
Type of RNA virus which should be transcribed first into positive sense RNA virus.
Negative sense RNA virus
Bronchiolitis
RSV
Infectious RNA
Hamstring Myalgia
Poliovirus
Influenza B and C infects…
Human only
HPV genes implicated in carcinogenesis
E6, E7 genes
Non paralytic polio
Aseptic meningitis
Australian antigen
Hepatitis B
True or false : genetic material of viruses may be both DNA and RNA.
False. Never both
Intranuclear inclusion bodies seen in poliovirus infection
Cowdry B
Aka slow virus
Lentivirus
Only occurs in inflenza A because of trading of RNA segments between animals and human strains. It is a reassortment, a major changes of the HA and NA resulting in devastating influenza pandemics.
Antigenic shift
Difference between Orthomyxovirus and paramyxovirus
Combined HA and NA Glycoprotein (wherein in Orthomyxovirus they are separate)
Has fusion protein (F protein)
Treatment for poliovirus
Pleconaril - inhibits penetration
Salk vaccine - formalin-killed poliovirus injected subQ
Sabin vaccine (OPV) - attenuated polio virus is ingested
Small mutations resulting in minor changes in the antigenicity of NA or HA. This results in epidemics go the common flu.
Antigenic drift
HSV 8 transmission
Sexually transmitted especially in homosexual men
Influenza A infects..
Human and animals
physical separation of the viral nucleic acid from the outer structural components of the virion so that it can function.
Uncoating
Disease caused by b19 virus of parvovirus with characteristic “slapped cheek” rash.
Erythema infectiosum
Diseases caused by poxvirus
Smallpox
Molluscum contagiosum - small white bumps with central dimple (like wart). Often found in genital region.
Gamma group herpesvirus
EBV, HSV-8
Catarrhal
Measles virus
Recurrence of VZV leads to what disease?
Shingles - painful eruption of vesicles isolated in a single dermatome. The vesicles dry up and form crusts which disappears in about 3wks.
All DNA virus have linear DNA, except…
Papovavirus
Hepadnavirus
Entire infectious unit
Virion
Steps of viral replication
- Adsorption and penetration
- Uncoating
- Synthesis and assembly of viral products
- Release of virions from host cell (by lysis or by budding)
Most common viral cause of infant pneumonia
RSV
T or F: acute viral hepatitis never becomes chronic in hepatitis A
True
All RNA virus are enveloped, except…
PCR
Picornavirus
Calicivirus
Reovirus
Characteristic appearance of the vesicle rash in chickenpox
Dew drop lesion on the top of a rose petal
Herpesvirus causes this type of infection where in the virus migrates up to the nerves to sensory ganglia and reside there. It will be activated in peripheral skin
Latency
Focal micro glial nodules in rabies
Babes nodules
Arenaviruses
Lymphocytic choriomeningitis virus (LCM)
Lassa virus
Arthropod borne viruses (arbovirus)
Togavirus
Flavivirus
Bunyavirus
Dengue fever is a biphasic fever. What is its vector
Aedes mosquitoes
T or F: IFN does not protect virus-infected cell and not itself the antiviral agent
True
Forscheimer spots
Rubella virus
All DNA virus have icosahedral capsid, except…
Pox virus
Family of hepa G virus
Flavivirus
Enteric hepatitis C
Hepatitis E
Family of hepa C virus
Flavivirus
Type I or viral IFN
IFN alpha and beta
Most common poliomyelitis
Abortive poliomyelitis
Rhinovirus replicates better in what temperature
33 degrees centigrade
Steps in viral pathogenesis
- Viral entry
- Primary viral replication
- Viral spread
- Cellular injury
- Host immune responses
- Viral clearance
- Viral shedding
Papovavirus
Polyomavirus that causes Progressive multifocal leukoencephalopathy (PML) characterized by degenerative cns white matter disease
JC polyomavirus
Varicella is highly contagious. It causes what disease?
Chickenpox
DNA virus which is circular and ds. The complete strand not covalently closed circle and other strand is missing approximately 25% of its length
Hepadnavirus
Virulence factor / glycoproteins of Orthomyxovirus
Hemagglutinin (HA)
Neuraminidase (NA)
type II or immune interferon
IFN Gamma
Papovavirus that causes warts
HPV
HPV type that causes verucca vulgaris and condylomata accuminata (hallmark: koilocytes)
HPV type 6,11
causes the epidemic keratoconjunctivitis.
Adenovirus type 8
Diseases caused by coxsackie A
Herpangina - fever, sore throat and small red-based vesicles over the back of the patient’s throat
Hand-foot-and-mouth disease
Family of hepa B virus
Hepadnavirus
Most common viral cause of infant diarrhea
Rotavirus
Chemical treatment for genital warts
Podophyllin
Retroviruses
Oncovirus
Lentivirus
Diagnostic tests for EBV
Paul-bundle test
Heterophil antibody test (positive by 2wks of illness)
Monospot test
Infectious particles composed solely of protein with no detectable nucleic acid.
Prion
Diseases caused by bunyavirus
Encephalitis
Korean hemorrhagic fever
Hantavirus pulmonary syndrome
Muerto canyon virus
Xanthochromic CSF is a finding in …
Herpes encephalitis
HSV 6 transmission
Transmitted by saliva
Envelope is acquired from
Budding
Creutzfieldt-Jakob syndrome
Heidenheim dementia
90% die in 1year
Corneal transplant
Prion (13-3-3 protein)
Beta group herpesvirus
CMV, HSV-6
HSV 2 latency in …
Lumbar or sacral sensory ganglia
Alpha group herpes virus
HSV-1
HSV-2
VZV
HPV type that causes common warts
e.g. Verucca plantaris, skin warts
HPV type 1, 2, 4 and 7
Appears during early acute phase and disappears before HBcAg is gone
Hep B 37 Ag (HBeAg)
Influenza a treatment
Amantadine and rimantidine
Picornaviruses
Enterovirus: poliovirus, coxsackie A&B, echovirus, hepatitis A
Rhinovirus
Hydrophobia and foaming of mouth in rabies is due to
Laryngospasm
Vaccine for poxvirus
Vaccinia virus - an avirulent pox virus
Small infectious agents that cause diseases of plants.
Viroids
Do not give aspirin in children for fever,. You give what?
Acetaminophen
Diseases caused by HSV 2
Genital herpes
Neonatal herpes
Aseptic meningitis in neonates
Characteristic appearance in roseola
Nagayama spots - in uvopalatoglossal junction
HSV type lesions below the waist
HSV 2
All DNA virus are double stranded, except…
Parvovirus
Paramyxoviruses
Parainfluenza virus
RSV
Mumps virus
Measles virus (rubeola)
Japanese encephalitis vector
Culex mosquitoes
RNA virus
PCR TCORPR picornavirus Calicivirus Reovirus Toga virus Corona virus Orthomyxovirus Rhabdovirus Paramyxovirus Retrovirus
Mode of transmission of HSV-1
Direct contact of mucus membrane
All DNA virus replicates in nucleus, except…
Pox virus which replicates in cytoplasm
Hepatitis that enteric and for expectant mother
Hepatitis E
Paramyxovirus that has no HA and NA
RSV
Family of hepa E virus
Calicivirus
HSV 1 latency in …
Trigeminal ganglia
Incubation of Hepatitis A
14-45 days
Mumps virus has only one antigenic type. Therefore, the MMR vaccine is protective. What is the antigen?
“S” soluble antigen
Disease caused by parainfluenza virus characterized by strider and barking cough due to infection and swelling of larynx
Croup
Mononucleosis is a dse of young adults. As with viral infections, the lower the socioeconomic class, the earlier children are indeed and the milder disease. American teenagers living in high socioeconomic class with better sanitation are infected later in life through social contact such as kissing. Thus the reference to kissing’s disease. What virus causes this?
EBV
CMV infection recurrence in immunocompromised patients, especially bone marrow transplant patients
CMV pneumonitis
Filovirus that cause mucus membrane bleeding (hemorrhagic fever)
Ebola virus
Disease caused by HSV 8
Kaposi’s sarcoma
Castleman’s disease (lympho proliferative disorder)
Primary effusion lymphoma
RNA viruses that cause common cold.
Coronavirus and rhinovirus (picornavirus)
Herpesviruses has this effect, especially HSV 1&2, and VZV. This results in separation of epithelium and causes blisters.
Cytopathic effect - cause cell destruction
Transmission of hepatitis A and E
Fecal-oral route
HPV type that causes genital warts
HPV type 6,11,16,18
Hepatitis that only carries DNA polymerase within the virion
Hepatitis B
RNA virus that contains nonfunctional ribosomes on its surface.
Arenavirus
Treatment fro HSV 1&2 and VZV
Acyclovir
Smallest virus
Picornavirus, parvovirus
Characterized by high fever, black vomitus, jaundice
Yellow fever
Family of hepa A virus
Picornavirus
Hepatitis that is asymptomatic, alone and acute
Hepatitis A
Cannibalism-related prion disease
Kuru “shivering”
Coxsackie A or B:
In mice, it causes paralysis and death with extensive skeletal muscle necrosis
Coxsackie A
Wheel-shape appearance
Rotavirus
DNA virus
HHAPPPy , PHP AHP Parvovirus Hepadnavirus Papovavirus Adenovirus Herpes virus Poxvirus
Herpes viruses
Herpes simplex virus type 1 and 2 HSV
Varicella-zoster virus VZV
cytomegalovirus CMV
Epstein-Barr virus EBV
Enveloped viruses are heat labile
True
Paramyxovirus that only has HA and F protein
Measles (rubeola)
Disease caused by HSV 6
Roseola infantum (6th disease, exanthem subitum)
All RNA virus have helical capsid symmetry, except…
PCRFT, R Picornavirus Calicivirus Reovirus Flavivirus Togavirus Rhabdovirus - bullet-shaped
Host cell outcome
- Death
2. Transformation - oncogene
RNA virus that cannot replicate without hepa B
Deltavirus
Disassociation of Dane particles leaves..
HBcAg and HBsAg
Eosinophilic intracytoplasmic inclusion in rabies virus
Negri bodies
HPV type that causes cervical cancer
HPV type 16,18
Antigen found within nuclei of infected hepatocytes
HBcAg
Mode of transmission of HSV-2
Sexually transmitted
Incubation of hepatitis B
50-180 days
Diseases caused by b19 strain of parvovirus
Erythema infectiosum
Transient aplastic anemia crisis
Disease caused by influenza virus
Flu fever
Transmission of Hepatitis B, C, D
Blood transfusion
Needle stick
Sexual
Across placenta
RNA virus that causes rabies
Rhabdovirus
Major target cell of EBV
B lymphocytes, binding to C3d receptor
HSV type lesions above the waist
HSV 1
Recurrent infection of measles after 2 years
Subacute sclerosing para encephalitis (SSPE)
What are the papovaviruses?
Papillomavirus (HPV)
Polyomavirus
Vacuolating viruses (SV40)
Bunyavirus that is not arbovirus
Hantavirus
Filoviruses
Ebola virus
Marburg virus
Togavirus that is not arbovirus
Rubivirus
DNA virus that causes childhood URTI such as rhinitis, sore throat, fever and conjunctivitis, and the epidemic keratoconjunctivitis (pink eye)
Adenovirus
Recurrent infection of HSV 1
Herpes labialis (fever blisters,cold sore)
Hematologic hallmark of Monocucleosis
Atypical lymphocytes
Autosomal dominant prion disease
Supranuclear gaze palsy
Gerstmann- straussler disease
Virus particle that is functionally deficient in some aspect for replication
Defective virus
All RNA virus replicates in cytoplasm, except…
Retrovirus
Orthomyxovirus
Segmental viruses
BORA bunyavirus Orthomyxovirus Reovirus Arenavirus
Intranuclear inclusion bodies seen in herpes encephalitis
Cowdry A
RNA virus that cause rubella (German measles/ 3-day measles)
Rubivirus
Vector of yellow fever virus
Aedes mosquito
Glycoprotein that binds with sialic acid, often found in the surface of RBC.
HA
Vaccine for rubella
MMR vaccine
Measles
Mumps
Rubella
Host-coded proteins that are member of cytokines and which inhibits viral replication.
Interferon (IFN)
Hepatitis that is defective and dependent to hepatitis B
Hepatitis D
Protein coat of viruses
Capsid
Diseases caused by flavivirus
Yellow fever Dengue fever Japanese encephalitis St. Louis encephalitis West Nile encephalitis Hepatitis c
Orthomyxovirus
Influenza virus (a,b,c)
Togaviruses
Alpha virus:
WEE
VEE
EEE
Rubivirus
Neuraminidase inhibitors that can shorten course of influenza A and B infection
Zanamivir (inhaled)
Oseltamivir (oral)
Soluble component of HBcAg and a markers of active disease and highly infectious
HBeAg
HPV type that causes laryngeal warts
HPV type 6,11
Incubation period of poliovirus
7-14 days or 3-35days
Diseases caused by EBV
BHINK
Burkitt’s lymphoma: B cell lympho affecting children in central Africa
Hodgkin’s disease : lymphoma characterized by arising in a single node or chain of nodes, spreading in anatomically contagious nodes
Infectious mononucleosis
Nasopharyngeal carcinoma
Kissing’s disease
Phase of viral growth cycle where in soon after interaction with a host cell, the infecting virion is disrupted and its measurable infectivity is lost.
Eclipse period
Disease that travels to the cns in a retrograde fashion up to the nerve axon
Rhabdovirus
Parvovirus that requires helper virus to replicate
(+) and (-) strand DNA are carried on separate particles
Defective parvovirus
Complication of influenza virus infection
Secondary bacterial pneumonia in elderly
Reye’s syndrome: children who use aspirin and get liver and brain disease
Hematologic hallmark in CMV
Owl’s eye inclusion
Diseases caused by mumps virus
Parotitis
Orchitis (in post pubertal male
Meningitis
Encephalitis
T or F: maternal antibody against mumps virus provide protection during 6months of life
True
T or F: Icosahedral viruses tend to be stable, losing little infectivity after several hours at 37 °C.
True
Incubation of mumps virus
18-21 days
The koplik’s spot in measles occur after 1-2days of prodrome. It is small red based blue-white centered lesions in the mouth found specifically in..
Opposite the 2nd upper molar
DNA virus that has brick-like appearance
Poxvirus
Sandy appearance
Arenavirus
Hepatitis that is blood-borne, big, bad, body-fluid
Hepatitis B
Typical lesion of staph aureus
Abscess
Tetracycline inhibits what ribosomal unit?
30s
Growth media which is the source of AA and N (beef,yeast extracts)
An undefined medium because AA source contains variety of cmpds with the exact composition being unknown
Contains all the elements that most bacteria needed for growth and are non selective
Nutrient agar
Selective and differential bacterial media:
Thayer-Martin or New York City agar
Neisseria with area of normal flora
Selective and differential bacterial media:
Buffered charcoal-yeast extract (BCYE) agar
Legionella
Ribosome subunits of fungi, Protozoa, helminths, animals and humans
80s
Exotoxins that binds to MHC II receptors
Non specifically activates large number of T cells
Superantigen exotoxins
Examples of differential media
Blood agar Eosin methylene blue EMB MacConkey Mannitol salt agar MSA X-gal plates
Staph that causes UTI in sexually active women
2nd to e.coli
Staph saprophyticus
Tears, saliva, and mucus has natural antimicrobial property
This enzyme cleaves the peptidoglycan backbone by breaking GLYCOSYL BOND
Lysozyme
Strep pneumoniae is encapsulated thus exhibits what reaction
Quellung reaction
Reserve of high energy stored in the form POLYMERIZED METAPHOSPHATE.
Volutin granules
(-) peptidoglycan
(+) sterols
Eukaryotes
Treatment for strep saprophiticus
Quinolones
Trimethoprim-sulfamethoxazole
Enriched media in which nutritionally rich whole blood supplements is the basic nutrient.
Blood agar
Disease caused by staph epidermidis
IV catheter infections
Endocarditis on normal prosthetic heart valves
Requires living host cells for growth thus they are the only obligatory intracellular organism among bacteria
Rickettsia and chlamydia
Enterococci is resistant to vancomycin because of different cross-linking sequence in the cell wall of the bacteria
D-ala-lactate
Enzymes of beta hemolytic
Streptolysin o and S (hemolysin)
Processes of DNA transfer on bacteria
Conjugation, transformation, transduction
The energy for flagellar movement provided by ATP
Proton-motive force
Enriched media that contains heat-treated blood (40-45c) which turns brown
Chocolate agar
Differential media for lactose fermentation
Macconkey
Nutrient agar medium composition
Beef extract- 0.3g (mineral and carbo)
A membrane disrupting bacterial exotoxins which contain lecithinase showing a double zone of hemolysis.
Cl. Perfringens alpha toxin
Basis of the selective action of several antibiotics
Differences in ribosomal RNAs and proteins
Inclusion bodies formed when source of N, S, P are depleted and there is excess C
Poly beta hydroxybutyric acid (PHB acid)
Green zone, incomplete hemolysis
Alpha hemolytic
Growth at 4C
Y. Enterocolitica
L. Monocytogenes
Treatment for staph aureus
Prp
Vancomycin (if methicillin or nafcillin resistant)
Clindamycin
Nafcillin or cloxacillin (if beta lactamases resistant)
Backbone of peptidoglycan
Alternating n-acetylglucosamine and N-acetylmuramic acid molecules
Transfer of naked forms of DNA which occurs in same sp of bacteria
Transformation
Enzyme reaction that produces O2 + H + NADP
NADPH oxidase
Capsule of bacillus anthracis is not composed of polysaccharide, it is composed of..
Glutamic acid
Linear pcs of DNA
Exogenates
Murein or mucopeptide
Structural support and maintains the characteristic shape
Able to withstand media of low osmotic pressure
Peptidoglycan
Transport media that uses broth for strict anaerobes
Thioglycolate
Type minimal media the contains a single selected agent, usually AA or sugar.
This allows the culturing of specific lines of auxotrophic recombinants
Supplementary minimal media
Different types of media for growing different types of cell
Differential media Enriched media Nutrient media Transport media Selective media
Diseases caused by staph aureus (6)
Mastitis Blepharitis Cellulitis Osteomyelitis Food poisoning TSS
Spore-forming bacteria
Clostridium and bacillus
Flagellum-like structure that provides undulating motion for spirochetes
Axial filament
Normal flora of conjunctiva
Diptheroids, staph epidermidis and non hemolytic strep
Found in the outer layer of gram positive bacteria
Antigenic, induce ab that are SPECIES-SPECIFIC
mediates the adherence of staph to mucosal cells
Teichoic acid
Toxin of vibrio cholerae
Catalyzes ADP-ribosylation of Gs protein ECG activates adenylcyclase that produces high level of cAMP, thus increases water and acid secretion
Choleragen
Most common resident of upper respiratory tract
Alpha hemolytic strep and neisseria
A selective differential agar used to isolate and identify member of enterococcus
Bile esculine agar
Coagulase (-)
Staph epidermidis and staph saprophyticus
Selective and differential bacterial media:
Loeffler’s and tellurite medium
Corynebacterium diptheria
Growth media used for the growth of only selected microorganisms. An antibiotic (by which the selective microorganisms is resistant) is added to the medium in order to prevent other cells, which do not posses the resistance, from growing.
Selective media
Growth media that contains minimum nutrients possible for colony growth.
Often used to grow wild type microorganism.
Used to select or used against recombinants or exconjugants
Minimal media
Selective and differential bacterial media:
TCBS (alkaline medium)
Vibrio cholerae
Transport media for vibrio cholera
Venkat-ramakrishnan medium
Evasion of immediate host defense:
Listeria
Listeriolysin
Components of anthrax toxin
Protective Antigen (PA) that serves as B component Edema factor (EF) that activates adenylcyclase Lethal factor (LF) kills cell
Teichoic acid is a polymer of (2)
Glycosyl phosphate and ribitol phosphate
Complete cell wall lysozyme digestion
Protoplast
Membrane disrupting exotoxins
Pore-forming cytolysin
Is cell wall or cell membrane antigenic?
Yes
Examples of defined media
Nutrient agar medium
Peptone
Agar
Bacteria that grow well in tap and distilled water
Psedomonasl
Toxins of staph aureus and its manifestations
Enterotoxin: vomiting, watery and nonbloody diarrhea
TSST: asso with tampon use, superantigen
Exfoliatin: phage II staph, scalded skin syndrome, superantigen
Alpha toxin: skin necrosis
Inhibits enolase
Fluoride ion
Microaerophilic
Campylobacter, helicobacter
Strep pyogenes has what type of capsule
Hyluronic acid capsule
Bacteria that escapes macrophage and grow inside them
RuMBLeS Rickettsia mycobacteria Brucella listeria shigella
Responsible for toxic effects of endotoxins. Found in LPS in OM.
Lipid A
Spores can be destroyed this steam heating
Autoclaving at 121C for 30 min, psi of 15
Peptone composition
- 5g protein and N source
0. 5g NaCl as electrolyte
Site of beta-lactamases
Periplasmic space
Pore forming cytolysin
Alpha toxin by staph aureus
Colonize with further spread
Strep. Pneumoniae
Neisseria gonorrheae and meningitidis
H. Influenzae
Mediates the adherence and phage typing in staph aureus
Teichoic acid
Examples of nutrient media
Blood agar
Chocolate agar
Acid-fast bacteria
Mycobacterial
Neurotoxin of Cl. Tetani which act on cns, inhibiting the inhibitory transmitter GABA
Tetanospasmin
Transport media that has a non-nutrient soft agar gel containing a reducing agent to prevent oxidation, charcoal to neutralize
Stuart transport medium
Transfer of bacterial genes via phage vectors
Transduction
Homologous recombination requires what protein
Rec A protein
Partial cell wall lysozyme digestion
Spheroplast
Strep pyogene is sensitive to..
Bacitracin
(+) peptidoglycan
(-) sterols
Prokaryotes
Neurotoxin of Cl. Botulinum that acts on peripheral synapses, blocking the release of neurotransmitters
Botulinum toxin
Selective and differential bacterial media:
EMB or MacConkey
Enteric bacteria
Hemolytic activity of strep pneumoniae
Alpha hemolytic
Obligate anaerobes
Actinomyces
Bacteroides
Clostridium
Enzyme reaction that produces H2O + O2
Catalase
Bacterium that is an obligate parasite
Treponema PALLIDUM
(-) teichoic acid; endotoxins
Gram negative bacteria
Test in lancefield classification
Precipitin test
Selective and differential bacterial media:
Chocolate agar
Haemophilus and neisseria for sterile area
Involved in cross-linking
D-alanine
Superantigen exotoxins examples
Toxic shock syndrome toxin (TSST) by staph aureus
Strep pyogenes exotoxin A (SPE-A)
In what phase in bacterial growth cycle when there is nutrient depletion or toxic products slow down growth until the number of new cells produced balances the cells that die
(Living=death)
Stationary phase
Minimal media contains
Carbon source (succinate)
Salts (Mg, N, P)
Water
(+) teichoic acid; exotoxin
Gram positive
Bacteria reproduce by this process in which one parent cell divides to form 2 progeny cells
Binary fission
Bacteria that has thick peptidoglycan
Gram (+) bacteria
In what phase in bacterial growth cycle when there is a marked decline on the number of viable bacteria.
Death phase
They have chitin, which is a polymer of N-acetylglucosamine
Fungi
A-B toxin that inhibits protein synthesis (5)
Diptheria toxin Exotoxin A Shigella toxin Shigalike toxin Verotoxin
Differential media mannitol fermentation
Mannitol salt agar
Gelatinous layer covering the entire bacterium
Composed of polysaccharide
Capsule
No hemolysis
Gamma hemolytic
Differential media that is used in strep test, contains bovine heart blood that becomes transparent in the presence of hemolytic streptococcus
Blood agar
Evasion of immediate host defense:
Mycoplasma
Ciliastasis
It a liquid or gel designed to support the growth of microorganisms or cells.
Growth medium or culture medium
Staph epidermidis is the normal flora of…
Skin ans mucus membrane
Selective and differential bacterial media:
Regan-Lowe
Bordetella pertussis
Evasion of immediate host defense:
Mycobacterium tuberculosis
Sulfolipids (sulfatides)
A single circular molecule of loosely organized DNA lacking a nuclear membrane and mitotic apparatus
Nucleiod
Obligate aerobes
Pseudomonas
Mycobacteirum
Component of exotoxin that binds to specific cell surface receptors and initiates internalization of the A component. It determines what cell type each toxin damages.
B component
What is the type of sex pili used for bacterial conjugation
Type VII
Treatment for staph epidermidis
Vancomycin
Produce igA protease
N. gonorrheae and meningitidis
H. Influenzae type B
Spores contains their own DNA
What structure Coats the spores?
Dipicolinic acid (calcium chelator)
Toxin of enterotoxic e.coli
Internalize A component to ADP-ribosylates Gs, which activates an adenylcyclase that produces high level of cAMP
Labile toxin
Strep pneumonia does not live in the presence of…
bile and optochin
Oxygen generates 2 toxic molecules
Hydrogen peroxide
Superoxide radicals
(+) sterols
Smallest bacteria
Mycoplasma
Mycoplasma genitalium (468 genes)
Bacteria infected by virus
Bacteriophage
Obligate intracellular pathogens
Mycobacterium leprae
Chlamydia
Mycoplasma
Peptidoglycan synthesis is inhibited by..
Penicillin and cephalosporin
Functions as the origin of the transverse septum that divides the cell on half and as the binding site of DNA bacterial ribosomes
Mesosome
Cross linking enzyme on the surface of bacteria which is targeted by beta-lactam antibiotics
Transpeptidase
Growth media that distinguishes one microorganism type to another.
This uses biochemical characteristics of the microorganism growing in the presence of specific nutrients or indicators
Differential or indicator media
Selective and differential bacterial media:
Hektoen enteric agar
Salmonella and shigella spa
One of the ways to visualize capsules, where it swells in the presence of homologous antibodies
Quellung reaction
A polysaccharide coating secreted by many bacteria
Adhere to various structures such as on the surface of teeth by strep.mutans
Glycocalyx or slime layer
Examples of transport media
Thioglycolate
Stuart transport medium
Venkat-ramakrishnan medium
They are determined by its rigid cell walls
Bacterial shape
Strep pyogenes causes what disease.
Pharyngitis
Replicate only within cells
Inner core of either DNA or RNA but no cytoplasm
Depends on host cells for protein synthesis and energy generation
Fast mutation
Virus
A-B toxin that inhibits protein synthesis:
Diptheria toxin
EF-2 inhibitor
Catalase (+)
Coagulase (+) forms golden yellow colonies
Mannitol (+)
Staph aureus
Component of exotoxin which internalize and inhibits a specific critical intracellular function causing damage to the cell. It is the active toxic portion of toxin
A component
Bacteria that invades the Peyer’s patches thru the phagocytic M cells
Shigella
Colonize with toxin elaboration
Corynebacterium diptheriae
Differential media for lac Operon mutants
X-gal plates
Mycobacteria is an acid fast bacteria, resisting decolorization with an acid alcohol after being stained by carbolfuchsin.
What component contributes to this property?
Mycolic acid
Exchange of two nearly identical pcs of DNA
Homologous recombination
Occurs when nutrients (c and n) are depleted
Sporulation
Staph aureus is a normal flora of..
Nose
Criteria of transport media (5)
1) temporary storage of specimens
2) maintains specimen’s viability without altering their conc
3) contains buffers and salt only
4) lacks C, N and other GF so as to prevent microbial multiplication
5) in isolating anaerobes, must be free from molecular oxygen
Lancet shape diplococci
Strep pneumoniae
Selective and differential bacterial media:
Lowenstein Jensen
Mycobacterium tuberculosis
Transfer of DNA directly from one living bacterium to another.
Bacterial sex.
Major mechanism for transfer of antibiotic resistance.
Conjugation
Most common bacterial resident of large intestine
Bacteroides
Contains gene for toxins and antibiotic resistance
Plasmid
Prokaryotes, especially bacteria doesn’t have true nucleus, rather they have..
NUCLEOID
Staph epidermidis is ____ sensitive
Novobiocin
Enzyme reaction that produces ClO + H2O
Myeloperoxidase
Determined group of beta hemolytic strep
C carbo
4 phases of bacterial growth cycle
1) lag phase
2) log phase
3) stationary phase
4) death phase
Functions of capsule
Virulence
Identification
Adherence
Antigen in vaccine
A-B toxin that inhibits protein synthesis:
Shiga toxin
Of shigella dysenteriae type I
A component cleaves 60s ribosomes
In what phase in bacterial growth cycle occurs a vigorous metabolic activity where cells do not divide yet.
Lag phase
In what phase in bacterial growth cycle when there is rapid cell division
Log phase
Major determinant of growth of bacteria
Nutrients
D-ala-D-ala is targeted by what antibiotic?
Vancomycin
Highly resistant structures formed in response to adverse conditions
Spores
Bacteria that grows in amoeba in streams
Legionella
Clear zone, complete hemolysis
Beta hemolytic
Axial filament.
Counterclockwise rotation - ?
Clockwise rotation -?
Counterclockwise rotation - directed motion
Clockwise rotation - tumbling
Undefined medium (aka basal or complex medium) contains: (3)
- C source
- Water
- Various salts
Invagination of cytoplasmic membrane
Mesosome
A-B toxin that increase cAMP
Labile toxin
Anthrax toxin
Pertussis toxin
Choleragen
Differential media for lactose and sucrose fermentation
EMB
Toxin that inhibits Gi which is the negative regulator of adenylcyclase thru ADP ribosylation thus increasing cAMP
Pertussis toxin
Enzyme reaction that produces H2O2 + O2
Superoxide dismutase
Determined by orientation and degree of attachment of bacteria at the time of cell division
Bacterial arrangement
True or false: pathologic effects of endotoxins are similar irrespective of organism
True
transport enzyme and oxidative phosphorylation
Plasma membrane
Ribosome subunit of bacteria
70s (50s and 30s)
A-B toxin that inhibits protein synthesis:
Verotoxin
Of e.coli serotype O157:H7
Inactivates protein synthesis by removing adenine from the 28s rRNA
Causes bloody diarrhea
Most important virulence factor of streps
Provides type specific immunity
M protein
Growth media commonly used to harvest as many different types of microbes as are present in the specimen.
Contains nutrients required to support the growth of wide variety of organisms.
Nutrient media
Erythromycin inhibits what ribosomal subunit of bacteria?
Inhibits 50s
Evasion of immediate host defense:
N. gonorrheae
Capsule
Agar composition
1.5g solidifying agent
100ml distilled water
pH 7
Breaks down the beta 1->4 bonds between NAM and NAG
Lysozyme
Group a strep
Strep pyogenes
Cytokines that cause fever
TNF, IL-1, IL-6
Facultative anaerobes
Strep
E. coli
Strep pneumoniae produces this enzyme causing mucosal colonization
IgA protease
Single, covalently closed circle or a loop of double stranded DNA
Bacterial chromosome
Defined medium (aka chemically defined medium or synthetic medium) contains:
- All chemical used are known
2. No yeast, animal or plant tissue present
Major protein in the cell wall of staph aureus which binds to Fc portion of IgG
Protein A
Evasion of immediate host defense: Some gram (-)
Membrane activation (MAC) resistance
Entamoeba, trichomonas
Diamond medium
Cryptosporidium
Sheather’s sugar flotation
Microsporidia
Ryan’s trichrome blue stain
Trichinosis
Bemtonite floccukation test
Schistosoma
Kato’s thick smear method
Acanthamoeba
Culbertson’s medium
Enterobius, taenia egg
Cellophane tape swab
Onchocerca volvulus
Mazzotti test
Leishmania
Montenegro skin test
Eosinophilia in urine
Hansel’s stain
Amoeba
Boeck and Orbohlav’s Locke-Egg serum medium
Strongyloides
Baermann’s technique
Filter paper strip procedure
Agar plate method
Trypanosoma cruzi
Machado-Guerreiro test
Trichomonas
Modified thioglycolate medium
Cyclospora
KOH
Egg counting
Stoll’s technique
Child + peri orbital cellulitis + sinusitis + ear discharge
H. Influenzae
Meyers-Kouwenaar syndrome is a synonym for
Tropical pulmonary eosiophilia
Occult filariasis
Weingartner’s syndrome
Army barracks + flu like+ maculopapular rash + petechiae
Meningococcemia (n.meningitidis)
Toxin of v.cholerae and LT enterotoxic of E. coli are similar
B subunits of the toxin bind to GM1 receptors on the host cell
Travel + fever + jaundice + bleeding + neurologic signs
Fulminant malaria (p.falciparum)
Diarrhea + beef, poultry + legumes
Clostridium perfringens
Watery diarrhea + shellfish ingestion
Vibrio cholera
Thumbprint sign + epiglottis
H.influenzae
Cellulitis + exposure to fresh water
Aeromonas hydrophila
Outbreaks of respiratory infections every winter
RSV
Diagnosis of infective endocarditis
Duke criteria
Outbreaks of respiratory infections in hospitalized infants
RSV
Diarrhea + fried rice ingestion + china town
Bacillus cereus
Camping + tick bite + flu like + blanching macules
Rocky Mountain spotted fever (rickettsia rickettsii)
Facial or sphenoidal sinus infection + unilateral + retro-orbital headaches
Septic cavernous sinus thrombosis (staph)
Pale, greasy, malodorous stools with malabsorption after drinking untreated stream or lake water
Giardia lamblia
Aseptic meningitis + facial diplegia
Borrelia burgdoferi
Bloody diarrhea + salami + apple juice + raw veggies
EHEC
Un pasteurized milk + cabbage
Listeria monocytogenes
Indolent + culture negative + a febrile + endocarditis
Tropheryma whipplei
HIV + hypoxemia + CXR infiltrates
Pneumocystis jiroveci
Para nasal infection + fever + nuchal rigidity + brainstem signs
Subdural empyema (microaerophilic staph and strep)
Diarrhea+ ingestion of potato + egg salad + mayonnaise
Staph aureus
Major determinant of virulence in h.influenzae
Capsule
Painless genital ulcer
Primary syphilis
Travel to US (Oklahoma/Arkansas/Missouri) + wild rabbits + tabanid fly
Tularemia
Watery diarrhea + salads + cheese
ETEC
Hemorrhagic vesicles surrounded by a rim of erythema with central necrosis or ulceration
Erythema gangrenosum (p.aeruginosa and A.hydrophila)
Ethmoid or maxillary sinus infection + nuchal rigidity + brain stem signs
Septic thrombosis of the superior Sagittal sinus (s.pneumoniae)
“Bull neck” appearance + pseudomembrane
Diphtheria
Quinolones kill bacteria by
Inhibiting DNA gyrase